LOCUS       sequence-335064-       11776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cell-free/mammalian expression vector for SARS-COV-2 virus NSP3 with
            N-term eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11776)
  TITLE     Alexandrov Lab Plasmids
REFERENCE   2  (bases 1 to 11776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pmCellFree_KA1_SARS-COV-2_NSP3
FEATURES             Location/Qualifiers
     source          1..11776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     236..1063
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             236..360
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(398..1003)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        1195..1498
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2023..2045
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2107..2126
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     2165..2184
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2165..2183
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2332..2355
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             2362..3072
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             2362..3072
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(2662..2681)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3009..3030
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3073..3096
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    3097..3121
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8960..8984)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9019..9036
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9126..9145)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9191..9246
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9245..9264)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      9334..9381
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     9394..10099
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           9482..9854
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(9511..10099)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     protein_bind    complement(10108..10141)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(10309..10896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10388..10407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10909..11700,11701..11769))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11532..11551
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctttttcaat attattgaag catttatcag ggttattgtc tcatgtccgc gcgtttcctg
       61 catcttttaa tcaaatccca agatgtgtat aaacgcgccg gtatgtacag gaagaggttt
      121 atactaaact gttacattgc aaacgtggtt tcgtgtgcca agtgtgaaaa ccgatgttta
      181 atcaaggctc tgacgcattt ctacaaccac gactccaagt gtgtgggtga agtcatgcat
      241 cttttaatca aatcccaaga tgtgtataaa ccaccaaact gccaaaaaat gaaaactgtc
      301 gacaagctct gtccgtttgc tggcaactgc aagggtctca atcctatttg taattattga
      361 ataataaaac aattataaat gtcaaatttg ttttttatta acgatacaaa ccaaacgcaa
      421 caagaacatt tgtagtatta tctataattg aaaacgcgta gttataatcg ctgaggtaat
      481 atttaaaatc attttcaaat gattcacagt taatttgcga caatataatt ttattttcac
      541 ataaactaga cgccttgtcg tcttcttctt cgtattcctt ctctttttca tttttctctt
      601 cataaaaatt aacatagtta ttatcgtatc catatatgta tctatcgtat agagtaaatt
      661 ttttgttgtc ataaatatat atgtcttttt taatggggtg tatagtaccg ctgcgcatag
      721 tttttctgta atttacaaca gtgctatttt ctggtagttc ttcggagtgt gttgctttaa
      781 ttattaaatt tatataatca atgaatttgg gatcgtcggt tttgtacaat atgttgccgg
      841 catagtacgc agcttcttct agttcaatta caccattttt tagcagcacc ggattaacat
      901 aactttccaa aatgttgtac gaaccgttaa acaaaaacag ttcacctccc ttttctatac
      961 tattgtctgc gagcagttgt ttgttgttaa aaataacagc cattgtaatg agacgcacaa
     1021 actaatatca caaactggaa atgtctatca atatatagtt gctgatggcc ggccgtaatg
     1081 agacgcacaa actaatatca caaactggaa atgtctatca atatatagtt gctctagtta
     1141 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     1201 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     1261 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     1321 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     1381 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     1441 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatgca
     1501 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
     1561 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
     1621 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     1681 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     1741 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     1801 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     1861 tccttcgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     1921 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggctgtc
     1981 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     2041 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     2101 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttggatcgga
     2161 cgtctaatac gactcactat agggacatct taagtttatt ttattttatt ttattttatt
     2221 ttattttatt ttattttatt ttattttatt ttattttatt taaccatgac agtaatgtat
     2281 aaagtctgta aagacattaa acacgtaagt gaaaccatgg gcacctctgg tcaccaccat
     2341 caccaccatc accacggcac aagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     2401 ctggtcgagt tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     2461 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     2521 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     2581 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     2641 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     2701 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2761 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     2821 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     2881 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2941 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     3001 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3061 gagctataca agctggaggt gctcttccag ggtccgacaa gtttgtacaa aaaagttggc
     3121 atggccccta ccaaggtgac cttcggagac gacaccgtga tcgaggtgca gggttacaag
     3181 agcgtgaaca tcaccttcga gctggacgaa cgcatcgaca aggtgctgaa cgaaaagtgc
     3241 tccgcttaca ccgtggagct gggcaccgag gtgaacgaat tcgcctgcgt ggtggccgac
     3301 gctgtgatca agaccctgca gccagtgtcc gaactgctga cccctctggg tatcgacctg
     3361 gacgagtggt ctatggctac ctactacctg ttcgacgaga gcggcgaatt caagctggcc
     3421 tctcacatgt actgcagctt ctaccctccc gacgaagacg aggaagaggg tgactgcgaa
     3481 gaggaagagt tcgagccttc cactcagtac gagtacggca ccgaagacga ctaccagggc
     3541 aagcctctgg aattcggagc cacctctgcc gctctgcagc ctgaagagga acaggaggaa
     3601 gactggctgg acgacgactc ccagcagacc gtgggtcagc aggacggctc tgaagacaac
     3661 cagaccacca ccatccagac catcgtggag gtgcagcctc agctggagat ggaactgacc
     3721 ccagtggtgc agaccatcga ggtgaacagc ttctccggct acctgaagct gaccgacaac
     3781 gtgtacatca agaacgctga catcgtggag gaagccaaga aggtgaaacc aaccgtggtg
     3841 gtgaacgccg ctaacgtgta cctgaagcac ggcggaggtg tggctggagc tctgaacaag
     3901 gctaccaaca acgccatgca ggtggaatcc gacgactaca tcgctaccaa cggtcctctg
     3961 aaggtgggcg gatcctgcgt gctgtctggc cacaacctgg ccaagcactg cctgcacgtg
     4021 gtgggaccaa acgtgaacaa gggagaggac atccagctgc tgaagagcgc ctacgaaaac
     4081 ttcaaccagc acgaggtgct gctggctcca ctgctgtctg ccggaatctt cggtgctgat
     4141 cccatccaca gcctgcgcgt gtgcgtggac accgtgagga ccaacgtgta cctggccgtg
     4201 ttcgacaaga acctgtacga caagctggtg agctccttcc tggagatgaa gtccgaaaag
     4261 caggtggagc agaagatcgc cgagatcccc aaggaggaag tgaagccttt catcaccgaa
     4321 tctaagccca gcgtggagca gagaaagcag gacgacaaga agatcaaggc ctgcgtggag
     4381 gaagtgacca ccaccctgga ggaaaccaag ttcctgaccg agaacctgct gctgtacatc
     4441 gacatcaacg gcaacctgca ccctgactcc gctaccctgg tgtctgacat cgacatcacc
     4501 ttcctgaaga aggacgcccc ctacatcgtg ggcgacgtgg tgcaggaagg agtgctgacc
     4561 gctgtggtca tccctaccaa gaaggccggt ggcaccaccg agatgctggc caaggctctg
     4621 cgtaaggtgc caaccgacaa ctacatcacc acctaccccg gtcagggcct gaacggttac
     4681 accgtggagg aagctaagac cgtgctgaag aagtgcaagt ctgccttcta catcctgccc
     4741 tccatcatct ctaacgagaa gcaggaaatc ctgggcaccg tgagctggaa cctgcgcgaa
     4801 atgctggccc acgctgagga aaccaggaag ctgatgcccg tgtgcgtgga gaccaaggcc
     4861 atcgtgagca ccatccagag aaagtacaag ggcatcaaga tccaggaggg tgtggtggac
     4921 tacggcgctc gtttctactt ctacacctcc aagaccaccg tggcctctct gatcaacacc
     4981 ctgaacgacc tgaacgagac cctggtgacc atgccactgg gatacgtgac ccacggtctg
     5041 aacctggagg aagccgctag atacatgcgt tccctgaagg tgcctgctac cgtgagcgtg
     5101 agcagccctg acgctgtgac cgcctacaac ggatacctga cctcctctag caagacccct
     5161 gaggaacact tcatcgaaac catcagcctg gccggttcct acaaggactg gtcttacagc
     5221 ggacagtcca cccagctggg catcgagttc ctgaagcgcg gagacaagag cgtgtactac
     5281 acctccaacc caaccacctt ccacctggac ggtgaagtga tcaccttcga caacctgaag
     5341 accctgctgt ctctgcgcga ggtgaggacc atcaaggtgt tcaccaccgt ggacaacatc
     5401 aacctgcaca cccaggtggt ggacatgagc atgacctacg gacagcagtt cggtcctacc
     5461 tacctggacg gagctgacgt gaccaagatc aagccacaca actcccacga gggcaagacc
     5521 ttctacgtgc tgcctaacga tgacaccctg agagtggagg ctttcgaata ctaccacacc
     5581 accgacccat ccttcctggg caggtacatg tctgccctga accacaccaa gaagtggaag
     5641 tacccccagg tgaacggact gaccagcatc aagtgggccg acaacaactg ctacctggcc
     5701 accgctctgc tgaccctgca gcagatcgaa ctgaagttca acccacccgc tctgcaggac
     5761 gcttactaca gagcccgtgc tggcgaggcc gctaacttct gcgctctgat cctggcctac
     5821 tgcaacaaga ccgtgggcga actgggagac gtgagagaga ccatgagcta cctgttccag
     5881 cacgccaacc tggactcctg caagcgtgtg ctgaacgtgg tgtgcaagac ctgcggccag
     5941 cagcagacca ccctgaaggg agtggaagct gtgatgtaca tgggtaccct gtcctacgag
     6001 cagttcaaga agggcgtgca gatcccttgc acctgcggaa agcaggccac caagtacctg
     6061 gtgcagcagg aaagcccatt cgtgatgatg tccgctcctc cagcccagta cgaactgaag
     6121 cacggcacct tcacctgcgc ttccgagtac accggcaact accagtgcgg acactacaag
     6181 cacatcacct ctaaggagac cctgtactgc atcgacggag ccctgctgac caagtcctct
     6241 gaatacaagg gtcccatcac cgacgtgttc tacaaggaga acagctacac caccaccatc
     6301 aagcccgtga cctacaagct ggacggcgtg gtgtgcaccg aaatcgaccc taagctggac
     6361 aactactaca agaaggacaa ctcttacttc accgagcagc ctatcgacct ggtgccaaac
     6421 cagccctacc ctaacgccag cttcgacaac ttcaagttcg tgtgcgacaa catcaagttc
     6481 gctgacgacc tgaaccagct gaccggatac aagaagcctg cctctagaga gctgaaggtg
     6541 accttcttcc cagacctgaa cggcgacgtg gtggctatcg actacaagca ctacacccca
     6601 agcttcaaga agggagccaa gctgctgcac aagcccatcg tgtggcacgt gaacaacgct
     6661 accaacaagg ccacctacaa gcctaacacc tggtgcatcc gttgcctgtg gtctaccaag
     6721 ccagtggaaa cctccaactc tttcgacgtg ctgaagagcg aggacgctca gggcatggac
     6781 aacctggcct gcgaagacct gaagcccgtg agcgaggaag tggtggagaa cccaaccatc
     6841 cagaaggacg tgctggaatg caacgtgaag accaccgagg tggtgggaga catcatcctg
     6901 aagccagcta acaacagcct gaagatcacc gaggaagtgg gtcacaccga cctgatggcc
     6961 gcttacgtgg acaacagctc cctgaccatc aagaagccca acgagctgag cagggtgctg
     7021 ggtctgaaga ccctggctac tcacggtctg gctgctgtga actctgtgcc atgggacacc
     7081 atcgctaact acgccaagcc tttcctgaac aaggtggtgt ccaccaccac caacatcgtg
     7141 accagatgtc tgaaccgcgt gtgcaccaac tacatgccct acttcttcac cctgctgctg
     7201 cagctgtgca ccttcaccag aagcaccaac tcccgtatca aggctagcat gcctaccacc
     7261 atcgccaaga acaccgtgaa gagcgtggga aagttctgcc tggaggcttc cttcaactac
     7321 ctgaagtctc caaacttcag caagctgatc aacatcatca tctggttcct gctgctctcc
     7381 gtgtgcctgg gttccctgat ctactctacc gccgctctgg gagtgctgat gtctaacctg
     7441 ggtatgccta gctactgcac tggttacaga gagggatacc tgaacagcac caacgtgacc
     7501 atcgccacct actgcaccgg cagcatccca tgctccgtgt gcctgtctgg actggacagc
     7561 ctggacacct acccctccct ggaaaccatc cagatcacca tctctagctt caagtgggac
     7621 ctgaccgcct tcggtctggt ggctgagtgg ttcctggcct acatcctgtt caccaggttc
     7681 ttctacgtgc tgggcctggc cgctatcatg cagctgttct tctcctactt cgctgtgcac
     7741 ttcatcagca actcctggct gatgtggctg atcatcaacc tggtgcagat ggctcccatc
     7801 tctgccatgg tgcgcatgta catcttcttc gccagcttct actacgtgtg gaagtcctac
     7861 gtgcacgtgg tggacggttg caactcctct acctgcatga tgtgctacaa gcgcaacagg
     7921 gctaccaggg tggagtgcac caccatcgtg aacggcgtgc gcaggtcctt ctacgtgtac
     7981 gccaacggcg gcaagggatt ctgcaagctg cacaactgga actgcgtgaa ctgcgacacc
     8041 ttctgcgctg gctccacctt catctctgac gaggtggcca gagacctgag cctgcagttc
     8101 aagcgtccaa tcaaccccac cgaccagagc tcctacatcg tggactctgt gaccgtgaag
     8161 aacggtagca tccacctgta cttcgacaag gctggccaga agacctacga gcgccactct
     8221 ctgagccact tcgtgaacct ggacaacctg agggccaaca acaccaaggg cagcctgcct
     8281 atcaacgtga tcgtgttcga cggaaagtcc aagtgcgagg aatctagcgc caagtccgcc
     8341 agcgtgtact acagccagct gatgtgccag ccaatcctgc tgctggacca ggctctggtg
     8401 tctgacgtgg gagacagcgc cgaagtggcc gtgaagatgt tcgacgccta cgtgaacacc
     8461 ttctcctcta ccttcaacgt gcctatggag aagctgaaga ccctggtggc tactgctgag
     8521 gctgaactgg ctaagaacgt gtccctggac aacgtgctga gcaccttcat ctccgccgct
     8581 aggcagggtt tcgtggactc tgacgtggaa accaaagacg tggtggagtg cctgaagctg
     8641 tctcaccaga gcgacatcga agtgaccggc gactcctgca acaactacat gctgacctac
     8701 aacaaggtgg agaacatgac cccaagagac ctgggagctt gcatcgactg ctccgcccgt
     8761 cacatcaacg ctcaggtggc caagtctcac aacatcgccc tgatctggaa cgtgaaggac
     8821 ttcatgagcc tgtccgagca gctgcgcaag cagatcagga gcgccgctaa gaagaacaac
     8881 ctgcccttca agctgacctg cgctaccacc agacaggtgg tgaacgtggt gaccaccaag
     8941 atcgccctga agggcggata cccaactttc ttgtacaaag tggtgtaagc ggccgccagc
     9001 tttctagacc agtttaaaca tcaccatcac catcactaag tgattaacct caggtgcagg
     9061 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag
     9121 atcgatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
     9181 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     9241 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
     9301 gtttagagtt tggcaacata tgcccatatg taactagcat aaccccttgg ggcctctaaa
     9361 cgggtcttga ggggtttttt gctgaaagca tgcggaggaa attctccttg aagtttccct
     9421 ggtgttcaaa gtaaaggagt ttgcaccaga cgcacctctg ttcactggtc cggcgtatta
     9481 aaacacgata cattgttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt
     9541 tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta
     9601 tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc
     9661 ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc
     9721 gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag
     9781 cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc
     9841 gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact
     9901 cgacgtaaac acgttaaata gagcttggac atatttaaca tcgggcgtgt tagctttatt
     9961 aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt
    10021 tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt
    10081 agttgagctt tttggaattg cgatcgcata acttcgtata gcatacatta tacgaagtta
    10141 taagctcgga acgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    10201 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    10261 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10321 ccgccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    10381 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    10441 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    10501 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    10561 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    10621 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    10681 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    10741 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    10801 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    10861 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgtt accaatgctt
    10921 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10981 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    11041 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    11101 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11161 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11221 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11281 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11341 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11401 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11461 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11521 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11581 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11641 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11701 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11761 aatactcata ctcttc
//
