LOCUS       sequence-335067-        2721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic bacterial terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pL3S2P21_STD
FEATURES             Location/Qualifiers
     source          1..2721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(18..37)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(19..37)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(44..61)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(44..60)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      87..542
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(174..193)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     384..405
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        620..724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(725..793,794..1585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(943..962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1756..2344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2245..2264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2498..2515
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2541..2557
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2541..2557
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2573..2591
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2573..2590
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gtcctaagcg gaattcaccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
       61 atgcgtttct acaatgttac gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg
      121 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      181 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      241 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      301 ttagggtgat ggttcacgta gtgggccagc gccctgatag acggtttttc gccctttgac
      361 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
      421 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
      481 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
      541 ttcctgatgc ggtattttat ccttacgcat ctgtgcggta tttcacaccg catcaggtgg
      601 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      661 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      721 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      781 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      841 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      901 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      961 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1021 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1081 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1141 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1201 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1261 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1321 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1381 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1441 gtcgcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1501 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1561 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1621 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1681 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1741 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1801 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1861 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1921 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1981 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2041 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2101 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2161 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2221 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2281 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2341 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2401 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2461 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2521 ggaaggtcat gatgcctgga caggaaacag ctatgaccaa cggctctatg gtatttaggt
     2581 gacactatag aacgaattca ctagtgattg ggcgatgagt ggtctcgcga actcggtacc
     2641 aaattccaga aaagaggcct cccgaaaggg gggccttttt tcgttttggt ccgcgacgag
     2701 accttgagat caatatctaa g
//
