LOCUS       sequence-335071-        4078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5-fluorocytosine sensitivity marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pFCU1
FEATURES             Location/Qualifiers
     source          1..4078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(174..965,966..1034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     797..816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1035..1139)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1217..1672)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1354..1375)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1566..1585
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(1698..1725)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     1866..1883
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1867..1883
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1890..1909
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1890..1908
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3115..3133)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3116..3133)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3149..3165)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3149..3165)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      3236..3307
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3322..3339)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3493..4078,1..3))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3573..3592)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
       61 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
      121 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
      181 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
      241 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
      301 gcaatgatac cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
      361 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
      421 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
      481 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
      541 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
      601 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
      661 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
      721 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
      781 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
      841 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
      901 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
      961 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1021 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1081 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     1141 acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag
     1201 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     1261 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     1321 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     1381 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcgc tggcccacta
     1441 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     1501 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     1561 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     1621 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgt
     1681 aacattgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
     1741 tggcagttta tggcgggcgt cctgcccgcc accctttcag ccaaaaaact taagaccgcc
     1801 ggtcttgtcc actaccttgc agtaatgcgg tggacaggat cggcggtttt cttttctctt
     1861 ctcaatgtaa aacgacggcc agtgaattgt aatacgactc actatagggt gaattccgct
     1921 taggacctta gatattgatc tcaaggtctc gaatggtgac cggcggcatg gccagcaagt
     1981 gggatcaaaa gggcatggat attgcctatg aggaggccgc cttgggctac aaggagggcg
     2041 gcgtccccat tggcggctgc ttgatcaaca acaaggatgg cagcgtgctg ggacgcggcc
     2101 acaacatgcg cttccagaag ggcagcgcca ccttgcacgg cgagatcagc accctggaga
     2161 actgcggacg cttggagggc aaggtgtaca aggataccac cctgtacacc accctgagcc
     2221 cctgcgatat gtgcaccggc gccatcatca tgtacggcat cccccgctgc gtggtgggcg
     2281 agaacgtgaa cttcaagagc aagggcgaga agtatctgca aacccgcgga cacgaggtgg
     2341 tggtggtgga tgatgagcga tgcaaaaaga tcatgaagca gttcatcgat gagcgccccc
     2401 aggattggtt cgaggatatt ggcgaggcca gcgagccctt caagaacgtg tatctgctgc
     2461 cccagaccaa ccaactgctg ggcctgtaca ccatcatccg caacaagaac accacccgcc
     2521 cagatttcat cttctattgt gaccgcatca tccgcctgct ggtcgaggag ggcctgaacc
     2581 acctgcccgt gcagaagcag atcgtcgaaa ccgataccaa cgagaacttc gagggcgtta
     2641 gcttcatggg caagatctgc ggcgtgagca tcgtgcgtgc cggcgagagc atggagcagg
     2701 gcctgcgcga ttgctgccgc agcgtgcgca tcggcaagat cctgatccag cgcgacgagg
     2761 aaaccgccct gcccaagctg ttttacgaga agctgcccga ggatatttcc gagcgctacg
     2821 tgttcctgct cgatcccatg ctggccaccg gcggcagcgc catcatggcc accgaggtgc
     2881 tgatcaagcg cggcgtgaag cccgagcgca tctactttct gaacctgatt tgcagcaagg
     2941 agggcattga gaagtaccac gccgccttcc cggaggtccg catcgtgacc ggagccttgg
     3001 atcgcggatt ggatgagaac aaatatctgg tgcccggcct gggcgatttc ggcgatcgct
     3061 actactgcgt gtaagcttcg agaccactca tcgcccaatc actagtgaat tcgttctata
     3121 gtgtcaccta aataccatag agccgttggt catagctgtt tcctgtcgga ccaaaacgaa
     3181 aaaaggcccc cctttcggga ggcctctttt ctggaatttg gtaccgagca ggcatcaaat
     3241 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     3301 cgctctctga ccttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3361 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat catgggataa
     3421 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3481 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3541 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3601 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3661 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3721 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3781 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3841 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3901 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3961 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4021 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatct
//
