LOCUS       sequence-335088-       16350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacteria gene circuit on low copy backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16350)
  AUTHORS   Zhang R, Goetz H, Melendez-Alvarez J, Li J, Ding T, Wang X, Tian XJ
  TITLE     Winner-takes-all resource competition redirects cascading cell fate 
            transitions.
  JOURNAL   Nat Commun. 2021 Feb 8;12(1):853. doi: 10.1038/s41467-021-21125-3.
  PUBMED    33558556
REFERENCE   2  (bases 1 to 16350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-02-8"; volume: "12(1):853. doi"; pages: " 
            10.1038/s41467-021-21125-3"
COMMENT     Sequence Label: pMMB206-CT81
FEATURES             Location/Qualifiers
     source          1..16350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        23..57
                     /label=BBa_J23116
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.16"
     RBS             66..77
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             84..704
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(157..176)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             705..737
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      785..856
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      872..899
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             1050..1061
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1647..1679
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      1727..1798
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1814..1841
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             1994..2005
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2012..2725
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGGTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2012..2725
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGGTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2042..2070)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2665..2685
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2732..2764
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      2787..2858
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2874..2901
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             3054..3065
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3072..3950
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      3972..4043
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4059..4086
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             4164..4175
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      4956..5027
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5043..5070
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             5148..5159
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5166..5840
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      5896..5967
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5983..6010
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             6088..6099
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6106..6984
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      7006..7077
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7093..7120
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    7127..7147
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(7159..7175)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7168..7190)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7345..7362)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      7515..7601
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7693..7720
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        7739..7830
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8273..8375
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             8376..9035
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8442..8461)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      9367..9761
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            10103..10190
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             11429..12400
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             12911..13750
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             13737..14588
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(14865..15947)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     15909..15928
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(15948..16025)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(16255..16260,16261..16276,16277..16283)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    16291..16307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16296..16318
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16325..16341
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16325..16341
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaattcgcgg ccgcttctag agttgacagc tagctcagtc ctagggacta tgctagctac
       61 tagagaaaga ggagaaatac tagatgtcca gattagataa aagtaaagtg attaacagcg
      121 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
      181 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
      241 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
      301 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
      361 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
      421 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
      481 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
      541 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
      601 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
      661 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa
      721 actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag
      781 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      841 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      901 ttatatacta gagacattga ttatttgcac ggcgtcacac tttgctatgc catagcaaga
      961 tagtccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct
     1021 ccattcccta tcagtgatag atactagaga aagaggagaa atactagatg actataatga
     1081 taaaaaaatc ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc
     1141 gttatcaagt gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat
     1201 cagatgagta tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg
     1261 taagtggatg ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc
     1321 ctgaattgct tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt
     1381 ttgctgtagg taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac
     1441 tatttgaagc tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa
     1501 catcaacagc aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag
     1561 acaaagaaat tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg
     1621 aacagtttaa aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt
     1681 aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa
     1741 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1801 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacatt
     1861 gattatttgc acggcgtcac actttgctat gccatagcaa gatagtccat aagattagcg
     1921 gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccg tttttttggg
     1981 ctagctacta gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg
     2041 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
     2101 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
     2161 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
     2221 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
     2281 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
     2341 tgaagtcaag tttgaaggtg gtacccttgt taatagaatc gagttaaaag gtattgattt
     2401 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
     2461 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
     2521 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     2581 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     2641 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     2701 acatggcatg gatgaactat acaaaaggcc tgctgcaaac gacgaaaact acgctttagt
     2761 agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     2821 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     2881 cttcgggtgg gcctttctgc gtttatatac tagagacatt gattatttgc acggcgtcac
     2941 actttgctat gccatagcaa gatagtccat aagattagcg gatcctacct gacgcttttt
     3001 atcgcaactc tctactgttt ctccataccg tttttttggg ctagctacta gagaaagagg
     3061 agaaatacta gatggctgaa gcgcaaaatg atcccctgct gccgggatac tcgtttaacg
     3121 cccatctggt ggcgggttta acgccgattg aggccaatgg ttatctcgat ttttttatcg
     3181 accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt cagggggtgg
     3241 tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga tattttgctg ttcccgccag
     3301 gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac cagtgggttt
     3361 actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaata tttgccaata
     3421 cgggtttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg tttgggcaaa
     3481 tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata aatctgcttg
     3541 agcaattgtt actgcggcgc atggaagcga ttaacgagtc gctccatcca ccgatggata
     3601 atcgggtacg cgaggcttgt cagtacatca gcgatcacct ggcagacagc aattttgata
     3661 tcgccagcgt cgcacagcat gtttgcctgt cgccgtcgcg tctgtcacat cttttccgcc
     3721 agcagttagg gattagcgtc ttaagctggc gcgaggacca acgcatcagc caggcgaagc
     3781 tgcttttgag cactacccgg atgcctatcg ccaccgtcgg tcgcaatgtt ggttttgacg
     3841 atcaactcta tttctcgcga gtatttaaaa aatgcaccgg ggccagcccg agcgagttcc
     3901 gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcataa taaactacta
     3961 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     4021 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     4081 tctgcgttta tatactagag acctgtagga tcgtacaggg ttacgcaaga aaatggtttg
     4141 ttatagtcga ataaatacta gagaaagagg agaaatacta gatgaaaaac ataaatgccg
     4201 acgacacata cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc
     4261 aatgcttatc tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt
     4321 atcctcattc tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga
     4381 ggcaatatta tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact
     4441 ccaatcattc accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc
     4501 caaatgtaat taaagaagcg aaaacagcag gtcttatcac tgggtttagt ttccctattc
     4561 atacggctaa caatggcttc ggaatactta gttttgcaca ttcagaaaaa gacaactata
     4621 tagatagttt atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg
     4681 ataattatcg aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag
     4741 aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat
     4801 taggttgcag tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata
     4861 caacaaaccg ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat
     4921 actttaaaaa ttaataaact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     4981 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     5041 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacctgt aggatcgtac
     5101 agggttacgc aagaaaatgg tttgttatag tcgaataaat actagagaaa gaggagaaat
     5161 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
     5221 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
     5281 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
     5341 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
     5401 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
     5461 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
     5521 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     5581 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     5641 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     5701 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     5761 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     5821 gtcgtcactc caccggtgct gctgcaaacg acgaaaacta cgcagcagca gtttaataat
     5881 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     5941 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     6001 cctttctgcg tttatatact agagacctgt aggatcgtac agggttacgc aagaaaatgg
     6061 tttgttatag tcgaataaat actagagaaa gaggagaaat actagatggc tgaagcgcaa
     6121 aatgatcccc tgctgccggg atactcgttt aacgcccatc tggtggcggg tttaacgccg
     6181 attgaggcca atggttatct cgattttttt atcgaccgac cgctgggaat gaaaggttat
     6241 attctcaatc tcaccattcg cggtcagggg gtggtgaaaa atcagggacg agaatttgtc
     6301 tgccgaccgg gtgatatttt gctgttcccg ccaggagaga ttcatcacta cggtcgtcat
     6361 ccggaggctc gcgaatggta tcaccagtgg gtttactttc gtccgcgcgc ctactggcat
     6421 gaatggctta actggccgtc aatatttgcc aatacgggtt tctttcgccc ggatgaagcg
     6481 caccagccgc atttcagcga cctgtttggg caaatcatta acgccgggca aggggaaggg
     6541 cgctattcgg agctgctggc gataaatctg cttgagcaat tgttactgcg gcgcatggaa
     6601 gcgattaacg agtcgctcca tccaccgatg gataatcggg tacgcgaggc ttgtcagtac
     6661 atcagcgatc acctggcaga cagcaatttt gatatcgcca gcgtcgcaca gcatgtttgc
     6721 ctgtcgccgt cgcgtctgtc acatcttttc cgccagcagt tagggattag cgtcttaagc
     6781 tggcgcgagg accaacgcat cagccaggcg aagctgcttt tgagcactac ccggatgcct
     6841 atcgccaccg tcggtcgcaa tgttggtttt gacgatcaac tctatttctc gcgagtattt
     6901 aaaaaatgca ccggggccag cccgagcgag ttccgtgccg gttgtgaaga aaaagtgaat
     6961 gatgtagccg tcaagttgtc ataataaact actagagcca ggcatcaaat aaaacgaaag
     7021 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     7081 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agtagcggcc
     7141 gctgcagcca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     7201 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     7261 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg atggctgttt
     7321 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct
     7381 gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa
     7441 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg
     7501 gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     7561 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     7621 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc
     7681 atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt
     7741 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7801 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     7861 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     7921 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7981 agcggtaaga tccttgagag ttttcgcccc gaagaacgtg atgtccggcg gtgcttttgc
     8041 cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca
     8101 ctggcctcaa aaacaccatc atacactaaa tcagtaagtt ggcagcatca cccgacgcac
     8161 tttgcgccga ataaatacct gtgacggaag atcacttcgc agaataaata aatcctggtg
     8221 tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc
     8281 acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg
     8341 agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     8401 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     8461 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     8521 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     8581 ctcatccgga attacgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     8641 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     8701 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     8761 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     8821 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     8881 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     8941 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     9001 aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattgtcttc
     9061 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     9121 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     9181 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     9241 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     9301 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccgtactaa
     9361 ctgtcacgaa cccctgcaat aactgtcacg cccccctgca ataactgtca cgaacccctg
     9421 caataactgt cacgccccca aacctgcaaa cccagcaggg gcgggggctg gcggggtgtt
     9481 ggaaaaatcc atccatgatt atctaagaat aatccactag gcgcggttat cagcgccctt
     9541 gtggggcgct gctgcccttg cccaatatgc ccggccagag gccggatagc tggtctattc
     9601 gctgcgctag gctacacacc gccccaccgc tgcgcggcag ggggaaaggc gggcaaagcc
     9661 cgctaaaccc cacaccaaac cccgcagaaa tacgctggag cgcttttagc cgctttagcg
     9721 gcctttcccc ctacccgaag ggtgggggcg cgtgtgcagc cccgcagggc ctgtctcggt
     9781 cgatcattca gcccggctca tccttctggc gtggcggcag accgaacaag gcgcggtcgt
     9841 ggtcgcgttc aaggtacgca tccattgccg ccatgagccg atcctccggc cactcgctgc
     9901 tgttcacctt ggccaaaatc atggccccca ccagcacctt gcgccttgtt tcgttcttgc
     9961 gctcttgctg ctgttccctt gcccgcaccc gctgaatttc ggcattgatt cgcgctcgtt
    10021 gttcttcgag cttggccagc cgatccgccg ccttgttgct ccccttaacc atcttgacac
    10081 cccattgtta atgtgctgtc tcgtaggcta tcatggaggc acagcggcgg caatcccgac
    10141 cctactttgt aggggagggc gcacttaccg gtttctcttc gagaaactgg cctaacggcc
    10201 acccttcggg cggtgcgctc tccgagggcc attgcatgga gccgaaaagc aaaagcaaca
    10261 gcgaggcagc atggcgattt atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca
    10321 atcggccagg gccaaggccg actacatcca gcgcgaaggc aagtatgccc gcgacatgga
    10381 tgaagtcttg cacgccgaat ccgggcacat gccggagttc gtcgagcggc ccgccgacta
    10441 ctgggatgct gccgacctgt atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt
    10501 tgccctgccg gtcgagctga ccctcgacca gcagaaggcg ctggcgtccg agttcgccca
    10561 gcacctgacc ggtgccgagc gcctgccgta tacgctggcc atccatgccg gtggcggcga
    10621 gaacccgcac tgccacctga tgatctccga gcggatcaat gacggcatcg agcggcccgc
    10681 cgctcagtgg ttcaagcggt acaacggcaa gaccccggag aagggcgggg cacagaagac
    10741 cgaagcgctc aagcccaagg catggcttga gcagacccgc gaggcatggg ccgaccatgc
    10801 caaccgggca ttagagcggg ctggccacga cgcccgcatt gaccacagaa cacttgaggc
    10861 gcagggcatc gagcgcctgc ccggtgttca cctggggccg aacgtggtgg agatggaagg
    10921 ccggggcatc cgcaccgacc gggcagacgt ggccctgaac atcgacaccg ccaacgccca
    10981 gatcatcgac ttacaggaat accgggaggc aatagaccat gaacgcaatc gacagagtga
    11041 agaaatccag aggcatcaac gagttagcgg agcagatcga accgctggcc cagagcatgg
    11101 cgacactggc cgacgaagcc cggcaggtca tgagccagac ccagcaggcc agcgaggcgc
    11161 aggcggcgga gtggctgaaa gcccagcgcc agacaggggc ggcatgggtg gagctggcca
    11221 aagagttgcg ggaggtagcc gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt
    11281 cgcgggggtg gcactggaag ctatggctaa ccgtgatgct ggcttccatg atgcctacgg
    11341 tggtgctgct gatcgcatcg ttgctcttgc tcgacctgac gccactgaca accgaggacg
    11401 gctcgatctg gctgcgcttg gtggcccgat gaagaacgac aggactttgc aggccatagg
    11461 ccgacagctc aaggccatgg gctgtgagcg cttcgatatc ggcgtcaggg acgccaccac
    11521 cggccagatg atgaaccggg aatggtcagc cgccgaagtg ctccagaaca cgccatggct
    11581 caagcggatg aatgcccagg gcaatgacgt gtatatcagg cccgccgagc aggagcggca
    11641 tggtctggtg ctggtggacg acctcagcga gtttgacctg gatgacatga aagccgaggg
    11701 ccgggagcct gccctggtag tggaaaccag cccgaagaac tatcaggcat gggtcaaggt
    11761 ggccgacgcc gcaggcggtg aacttcgggg gcagattgcc cggacgctgg ccagcgagta
    11821 cgacgccgac ccggccagcg ccgacagccg ccactatggc cgcttggcgg gcttcaccaa
    11881 ccgcaaggac aagcacacca cccgcgccgg ttatcagccg tgggtgctgc tgcgtgaatc
    11941 caagggcaag accgccaccg ctggcccggc gctggtgcag caggctggcc agcagatcga
    12001 gcaggcccag cggcagcagg agaaggcccg caggctggcc agcctcgaac tgcccgagcg
    12061 gcagcttagc cgccaccggc gcacggcgct ggacgagtac cgcagcgaga tggccgggct
    12121 ggtcaagcgc ttcggtgatg acctcagcaa gtgcgacttt atcgccgcgc agaagctggc
    12181 cagccggggc cgcagtgccg aggaaatcgg caaggccatg gccgaggcca gcccagcgct
    12241 ggcagagcgc aagcccggcc acgaagcgga ttacatcgag cgcaccgtca gcaaggtcat
    12301 gggtctgccc agcgtccagc ttgcgcgggc cgagctggca cgggcaccgg caccccgcca
    12361 gcgaggcatg gacaggggcg ggccagattt cagcatgtag tgcttgcgtt ggtactcacg
    12421 cctgttatac tatgagtact cacgcacaga agggggtttt atggaatacg aaaaaagcgc
    12481 ttcagggtcg gtctacctga tcaaaagtga caagggctat tggttgcccg gtggctttgg
    12541 ttatacgtca aacaaggccg aggctggccg cttttcagtc gctgatatgg ccagccttaa
    12601 ccttgacggc tgcaccttgt ccttgttccg cgaagacaag cctttcggcc ccggcaagtt
    12661 tctcggtgac tgatatgaaa gaccaaaagg acaagcagac cggcgacctg ctggccagcc
    12721 ctgacgctgt acgccaagcg cgatatgccg agcgcatgaa ggccaaaggg atgcgtcagc
    12781 gcaagttctg gctgaccgac gacgaatacg aggcgctgcg cgagtgcctg gaagaactca
    12841 gagcggcgca gggcgggggt agtgaccccg ccagcgccta accaccaact gcctgcaaag
    12901 gaggcaatca atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc
    12961 gccaccgctg gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc
    13021 gcccggtggt gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg
    13081 gccggatctg ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga
    13141 agacccgccc accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga
    13201 ggaacggcaa gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa
    13261 catcatggcc ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat
    13321 ggtgctggac acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc
    13381 ccaggtcatc ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct
    13441 gcaccatgcc agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg
    13501 gggcagctcg gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag
    13561 cgccgaggcc gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg
    13621 tgtgagcaag gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg
    13681 cggggtgctc aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg
    13741 tgaagcctaa gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg
    13801 cccccggcct gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga
    13861 cgtatgacta cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg
    13921 atgatctgcg catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg
    13981 gcccggaacc caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg
    14041 aggccgtcac cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa
    14101 tcggggcaga ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc
    14161 tttggaaggt atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt
    14221 cggagtacgc cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg
    14281 cgcaggccgt catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc
    14341 tggacagcga aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca
    14401 aaaccggcaa ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg
    14461 gttcgaccat gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc
    14521 tgggctggac ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg
    14581 caggctgacc ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc
    14641 ttattttcct gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa
    14701 tattttgaaa aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct
    14761 ttgcttccag atgtatgctc ttctgctccc gaacgccagc aagacgtagc ccagcgcgtc
    14821 ggccagcttg caattcgcgc taacttacat taattgcgtt gcgctcactg cccgctttcc
    14881 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    14941 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga
    15001 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
    15061 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg
    15121 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg
    15181 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
    15241 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc
    15301 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
    15361 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat
    15421 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg
    15481 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
    15541 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
    15601 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac
    15661 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
    15721 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc
    15781 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt
    15841 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa
    15901 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg
    15961 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg
    16021 gtgtcaacgt aaatgccgct tcgccttcgc gcgcgaattg caagctgatc cgggcttatc
    16081 gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct
    16141 gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
    16201 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca
    16261 attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    16321 acagcaggaa acagctatga ccatgattac
//
