LOCUS       sequence-33509-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Dittgen T, Nimmerjahn A, Komai S, Licznerski P, Waters J, Margrie 
            TW, Helmchen F, Denk W, Brecht M, Osten P
  TITLE     Lentivirus-based genetic manipulations of cortical neurons and their
            optical and electrophysiological monitoring in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Dec 28. 101(52):18206-11.
  PUBMED    15608064
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Dec 28. 101(52):18206-11."
COMMENT     Sequence Label: pFCK(1.3)tdimer2W
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..78
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     476..496
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(640..663)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(838..857)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aggcgaggcg agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc
       61 cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc
      121 aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc
      181 agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc
      241 cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat
      301 gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac
      361 aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat
      421 gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag
      481 cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag
      541 aggttgatta tcgataagct tgatatcgaa ttaattccta caggaacagg tggtggcggc
      601 cctcggagcg ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
      661 gcttggtgtc cacgtagtag tagccgggca gctgcacggg cttcttggcc atgtagatgg
      721 tcttgaactc caccaggtag tggccgccgt ccttcagctt cagggcctgg tggatctcgc
      781 ccttcagcac gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag cccatggtct
      841 tcttctgcat tacggggccg tcggggggga agttggtgcc gcggaacttc accttgtaga
      901 tcagcgtgcc gtcctgcatg ggaggagtcc tgggtcacgg tcaccacgcc gccgt
//
