LOCUS       sequence-335091-        2893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  QF DNA binding domain, requires assembly with TL2-TL4 linker and TL5
            activation domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pQFBD
FEATURES             Location/Qualifiers
     source          1..2893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..36
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(695..713)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(696..713)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(729..745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(729..745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(860..1448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(940..959)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1563..1665
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1666..2325
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1732..1751)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2438..2866
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2525..2544)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2735..2756
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacattgta gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag
       61 ggtgaattcc gcttaggacc ttagatattg atctcaaggt ctcgaatgcc ccccaagcgc
      121 aagactctga acgccgccgc tgaggccaac gcccacgctg acggtcatgc tgatggtaac
      181 gctgatggtc acgtcgccaa taccgctgct agctccaaca acgcccgttt cgccgacctg
      241 accaatattg atacccccgg cttgggcccc accaccacca ccctgctggt ggagcccgcc
      301 cgttccaagc gtcagcgtgt gtcccgcgcc tgcgaccagt gccgcgccgc tcgtgagaag
      361 tgcgacggaa tccagccagc ctgcttcccc tgcgtgtccc agggccgctc ctgcacttac
      421 caggcctccc caaaaaagcg cggcgtgcag actggataca ttcgcaccct cgagctcgcc
      481 ctggcctgga tgttcgagaa cgtggcccgc tccgaggatg ctctgcataa cctgttggtc
      541 cgtgacgccg gacagggctc ggccctgctg gtcggcaagg actcgcccgc cgctgagcgc
      601 ctgcatgctc gttgggccac cagccgtgtc aacaagagca tcacccgcct cctgagtgcg
      661 agaccactca tcgcccaatc actagtgaat tcgttctata gtgtcaccta aataccatag
      721 agccgttggt catagctgtt tcctgtccag gcatcatgac acggttatcc acagaatcag
      781 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      841 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      901 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      961 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1021 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1081 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1141 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1201 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1261 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1321 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1381 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1441 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1501 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttgt
     1561 gatgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     1621 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     1681 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     1741 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     1801 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     1861 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     1921 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     1981 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     2041 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtg
     2101 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     2161 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     2221 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     2281 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     2341 tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat
     2401 ggcagaaatt cgatgataag ctgtcaaaca tgatgttacg cgccctgtag cggcgcatta
     2461 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2521 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2581 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2641 aaaaaacttg attagggtga tggttcacgt agtgggccag cgccctgata gacggttttt
     2701 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     2761 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     2821 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     2881 acgcttacaa ttt
//
