LOCUS       sequence-335102-        8859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast two-hybrid vector using a copper inducible CUP1 promoter, 
            expressing full length Nxf1B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Hernandez J, Ross K, Hamilton B
  TITLE     Inducible Yeast Two-Hybrid with Quantitative Measures
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 8859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: pGBK CUP1 Nxf1B
FEATURES             Location/Qualifiers
     source          1..8859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..235
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     CDS             291..731
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     742..761
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        742..760
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             780..809
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      2699..2746
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2773..2960
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2828..2852)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2984..3000)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2984..3000)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2997..3019)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3008..3024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3032..3038,3039..3056,3057..3062))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3077..3098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3215..3232)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3386..3974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3466..3485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4303..4350
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4375..4394
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4582..5376)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4582..5376)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4693..4712)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5303..5322
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5377..5481)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5508..6850
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6471..6518)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     6891..6909
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6947..6969)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7069..7088
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7109..7390
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             7391..8065
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(8168..8623)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8305..8326)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8517..8536
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctagttagaa aaagacattt ttgctgtcag tcactgtcaa gagattcttt tgctggcatt
       61 tcttctagaa gcaaaaagag cgatgcgtct tttccgctga accgttccag caaaaaagac
      121 taccaacgca atatggattg tcagaatcat ataaaagaga agcaaataac tccttgtctt
      181 gtatcaattg cattataata tcttcttgtt agtgcaatat catatagaag tcatcgaaat
      241 agatattaag aaaaacaaac tgtccaagct tgaagcaagc ctcctgaaag atgaagctac
      301 tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag
      361 aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa
      421 ccaaaaggtc tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac
      481 tggaacagct atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg
      541 attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata
      601 aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta acattgagac
      661 agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt
      721 tgactgtatc gccggaattt gtaatacgac tcactatagg gcgagccgcc atcatggagg
      781 agcagaagct gatctcagag gaggacctgc atatggcgga cgaagggaag tcatacaacg
      841 agcatgatga ccgtgttagt ttccctcaaa gaagaaagaa aggccggggg cctttccgat
      901 ggaagtgtgg tgaggggaat cgtaggtctg gaagaggtgg ttctggtgta cagtcttccc
      961 gatttgagga agatgatggc gatgtagcaa tgaatgatcc ccaggatggc ccccgagtaa
     1021 gatacaatcc ctataccaac cggcctaatc gtcggggaga tggttggcat gatcgagatc
     1081 gaattcatat tactgtacgg agagacagag ctcctgcaga gagaggaggg gctggcacca
     1141 gtcaagatgg gaccaccaag aactggttca agattacaat tccttatggt agaaagtatg
     1201 acaaaacgtg gcttctgagc atgattcaga gcaaatgcag tgtccccttc aatcccatcg
     1261 agtttcacta tgaaaataca cgggcccatt tttttgtgga ggatgccact actgcttctg
     1321 cattaaaggg tgtcaaccat aagatccagg atcgagaaaa cagaaggata tctatcatca
     1381 tcaatgcttc tgctccaccc tacactgtac agaatgaact gaagcccgaa caaatagagc
     1441 agctcaagct gatcatgagc aaacgatatg atggcaacca acaagcactt gacctcaagg
     1501 ggctccgctc agacccagat ttggtggccc agaacatcga tgttgttcta aaccgcagaa
     1561 gctgtatggc agccaccctg cggatcattg aagagaacat tcctgagcta ttgtctttga
     1621 acttgagcag caacaggcta tacaagctgg atgatatgtc tagcattgtg cagaaggcgc
     1681 caaacctaaa gaccctaaat ctctccggaa acgaattgaa gactgagcgg gaattagaca
     1741 agataaaagg gctgaagctg gaagagctgt ggcttgacag aaaccccatg tgtgacaact
     1801 tcggagacca gtccagctat atcagcgcca ttcgcgaacg atttcccaag ttattacgcc
     1861 tggatggcca tgagctaccc cctccaattt cctttgatgt cgaagcccct acaatgttac
     1921 caccctgcaa gggaagctat tttggaaccg agaacctaaa gagtctggtc ctgcgcttct
     1981 tgcagcagta ctatgtaatt tatgactcag gagaccgcca gggtctcctg tatgcctacc
     2041 atgatggcgc ctgttgctcg ctaagcattc cttacaaccc tcagaatcct gtccgaaaaa
     2101 acttagctga atatgtcaaa gacagcagaa atgtgaagaa gcttaaagaa cccacccagc
     2161 ggttccgact cttaaagcat acacgtctta atgtcgttgc cttcctcaat gagttaccca
     2221 aaactcaaca tgacgttaac gccttcgtgg tagatataag tgcccaaaca agcacattac
     2281 tgtgtttttc tgtcaatgga gtcttcaagg aagtggatgg gaagtctcga gattctttac
     2341 gtgctttcac aagaacgttc attgctgttc ctgccagcaa ttcagggcta tgtattgtaa
     2401 atgatgaatt atttgtgcgg aatgccagcc ctgaagaaat ccaaagagcc ttcgccatgt
     2461 ctgcacccac accttcctcc agtccagtac ccacgctttc cccagaacag caggaaatgt
     2521 tgcaagcatt ttctacccag tctggcatga accttgagtg gtctcagaag tgccttcaag
     2581 acaataactg ggactacacc agatctgctc aggccttcac tcttctcaag gccaagggag
     2641 agattccaga agtagcgttt atgaaatgag gatccgtcga cctgcagcgg ccgcataact
     2701 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgcgcg cttgcagcca
     2761 agctaattcc gggcgaattt cttatgattt atgattttta ttattaaata agttataaaa
     2821 aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc
     2881 ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt
     2941 attgaccaca cctctaccgg catgcaagct tggcgtaatc atggtcatag ctgtttcctg
     3001 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     3061 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3121 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     3181 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3241 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3301 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3361 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3421 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3481 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3541 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3601 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3661 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3721 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3781 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3841 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3901 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3961 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4021 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4081 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta
     4141 tggcagggcc tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg
     4201 ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg
     4261 tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac
     4321 cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
     4381 ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc
     4441 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
     4501 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
     4561 tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     4621 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     4681 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     4741 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     4801 gcaggcatcg ccatgagtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct
     4861 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     4921 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     4981 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     5041 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     5101 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     5161 cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag
     5221 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     5281 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     5341 cggagaacct gcgtgcaatc catcttgttc aatcatactc ttcctttttc aatattattg
     5401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg
     5521 tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
     5581 tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa
     5641 tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     5701 gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca
     5761 aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa
     5821 caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata
     5881 actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc
     5941 ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg
     6001 tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat
     6061 actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg
     6121 gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt
     6181 ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag taatactaga
     6241 gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga
     6301 tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa
     6361 tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg
     6421 gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta
     6481 tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg
     6541 cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat
     6601 ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt
     6661 aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg
     6721 atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt
     6781 ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc atttcctttg
     6841 atattggatc atactaagaa accattatta tcatgacatt aacctataaa aataggcgta
     6901 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     6961 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     7021 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     7081 agattgtact gagagtgcac catagatcaa cgacattact atatatataa tataggaagc
     7141 atttaataga acagcatcgt aatatatgtg tactttgcag ttatgacgcc agatggcagt
     7201 agtggaagat attctttatt gaaaaatagc ttgtcacctt acgtacaatc ttgatccgga
     7261 gcttttcttt ttttgccgat taagaattaa ttcggtcgaa aaaagaaaag gagagggcca
     7321 agagggaggg cattggtgac tattgagcac gtgagtatac gtgattaagc acacaaaggc
     7381 agcttggagt atgtctgtta ttaatttcac aggtagttct ggtccattgg tgaaagtttg
     7441 cggcttgcag agcacagagg ccgcagaatg tgctctagat tccgatgctg acttgctggg
     7501 tattatatgt gtgcccaata gaaagagaac aattgacccg gttattgcaa ggaaaatttc
     7561 aagtcttgta aaagcatata aaaatagttc aggcactccg aaatacttgg ttggcgtgtt
     7621 tcgtaatcaa cctaaggagg atgttttggc tctggtcaat gattacggca ttgatatcgt
     7681 ccaactgcat ggagatgagt cgtggcaaga ataccaagag ttcctcggtt tgccagttat
     7741 taaaagactc gtatttccaa aagactgcaa catactactc agtgcagctt cacagaaacc
     7801 tcattcgttt attcccttgt ttgattcaga agcaggtggg acaggtgaac ttttggattg
     7861 gaactcgatt tctgactggg ttggaaggca agagagcccc gaaagcttac attttatgtt
     7921 agctggtgga ctgacgccag aaaatgttgg tgatgcgctt agattaaatg gcgttattgg
     7981 tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa gactctaaca aaatagcaaa
     8041 tttcgtcaaa aatgctaaga aataggttat tactgagtag tatttattta agtattgttt
     8101 gtgcacttgc cgatctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     8161 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     8221 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     8281 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     8341 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     8401 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     8461 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     8521 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     8581 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc
     8641 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagatcccat
     8701 taccgacatt tgggcgctat acgtgcataa gttcatgtat gtatctgtat ttaaaacact
     8761 tttgtattat ttttcctcat atatgtgtat aggtttatac ggatgattta attattactt
     8821 caccaccctt tatttcaggc tgatatctta gccttgtta
//
