LOCUS       sequence-335106-        3053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0-SP promoterless vector encoding 
            Neonothopanus nambi luciferase nnLuz codon-optimised for expression 
            in Nicotiana benthamiana and Pichia pastoris.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS
  TITLE     Plants with genetically encoded autoluminescence.
  JOURNAL   Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9.
  PUBMED    32341562
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9."
COMMENT     Sequence Label: pX018
FEATURES             Location/Qualifiers
     source          1..3053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(479..490)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(851..869)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        937..1041
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1263..2054
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2225..2813
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2714..2733
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2967..2984
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcaaat gaggataaat atctctttgt ctagtctctt tgagagactg agcaaattgt
       61 catcccgatc catcgcaatt acctgcggcg tagtcttggc aagtgcaata gcattcccaa
      121 ttatccgaag agactatcaa acgttccttg aagtcggtcc gagctatgcc ccacagaact
      181 tccgaggcta tatcatcgtt tgtgtcttgt cactttttag gcaagagcag aaagggttgg
      241 caatctatga caggcttcca gaaaagaggc gttggcttgc cgacttgccg tttcgtgaag
      301 ggacgaggcc atccataacc tcccacatta tacagcgtca gcgtactcag ctggtagatc
      361 aagagtttgc aaccagagaa cttatcgata aggtgatccc acgtgtgcaa gcaagacaca
      421 cagacaaaac ttttctcagt acgtcaaaat ttgagtttca tgcaaaggcc atcttccttc
      481 tcccctctat ccctattaat gatcctctta acattccctc acacgatacg gtaagaagaa
      541 ccaagcgtga gatcgctcac atgcatgatt accatgattg cactctgcac ttggctcttg
      601 ctgctcagga tggtaaggaa gttttgaaga aggggtgggg ccagcgtcac ccactggccg
      661 gaccaggagt cccaggccct cctactgagt ggaccttcct ttacgcacca aggaacgaag
      721 aggaggccag agttgtcgag atgatagtcg aagctagtat tggctacatg actaacgatc
      781 ctgctggtaa gattgtcgaa aatgccaagt caggttgaga ccacgaagtg gctcttcagt
      841 ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      901 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      961 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1021 taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     1081 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttttgggg
     1141 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     1201 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     1261 tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     1321 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     1381 gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta aggcttgatg
     1441 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     1501 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     1561 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     1621 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     1681 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     1741 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     1801 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     1861 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     1921 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     1981 cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag atcaccaagg
     2041 tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2101 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2161 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2221 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2281 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2341 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2401 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2461 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2521 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2581 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2641 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2701 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2761 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2821 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2881 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2941 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3001 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatcactc tgt
//
