LOCUS       sequence-335113-        2574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick - pCmYLCV911.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Gonzalez-Grandio E, Demirer GS, Ma W, Brady S, Landry MP
  TITLE     A Ratiometric Dual Color Luciferase Reporter for Fast 
            Characterization of Transcriptional Regulatory Elements in Plants.
  JOURNAL   ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248.
  PUBMED    34520169
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248."
COMMENT     Sequence Label: pUPD2+pCmYLCV911
FEATURES             Location/Qualifiers
     source          1..2574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      531..588
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(612..629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(783..1371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(863..882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1554..1648)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1669..2328)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2243..2262
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2490..2508
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2512..2555)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(2558..2574,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg ggagtggcag acatactgtc ccacaaatga agatggaatc
       61 tgtaaaagaa aacgcgtgaa ataatgcgtc tgacaaaggt taggtcggct gcctttaatc
      121 aataccaaag tggtccctac cacgatggaa aaactgtgca gtcggtttgg ctttttctga
      181 cgaacaaata agattcgtgg ccgacaggtg ggggtccacc atgtgaaggc atcttcagac
      241 tccaataatg gagcaatgac gtaagggctt acgaaataag taagggtagt ttgggaaatg
      301 tccactcacc cgtcagtcta taaatactta gcccctccct cattgttaag ggagcaaaat
      361 ctcagagaga tagtcctaga gagagaaaga gagcaagtag cctagaagta gtcaaggcgg
      421 cgaagtattc aggcaggtgg ccaggaagaa gaaaagccaa gacgacgaaa acaggtaaga
      481 gctaagctta atgtgagacc actcatcgcg actagtagcg gccgctgcag tccggcaaaa
      541 aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt
      601 atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      661 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      781 tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1201 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1441 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1501 aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca
     1561 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     1621 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
     1681 ctgccactcg tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     1741 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     1801 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     1861 aaaactggtg aaactcaccc agggattggc tgatacgaaa aacatattct caataaaccc
     1921 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     1981 aaactgccgg aaatcgtcgt ggtattcact ccagagtgat gaaaacgttt cagtttgctc
     2041 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     2101 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     2161 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     2221 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     2281 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     2341 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     2401 gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac gtctaagaaa
     2461 ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat
     2521 aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg cttc
//
