LOCUS       sequence-335128-        4953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Brunet A, Bonni A, Zigmond MJ, Lin MZ, Juo P, Hu LS, Anderson MJ, 
            Arden KC, Blenis J, Greenberg ME
  TITLE     Akt promotes cell survival by phosphorylating and inhibiting a 
            Forkhead transcription factor.
  JOURNAL   Cell 1999 Mar 19;96(6):857-68.
  PUBMED    10102273
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "1999-03-19"; volume: "96(6)"; pages: "857-68"
COMMENT     Sequence Label: HA-FOXO3a WT
FEATURES             Location/Qualifiers
     source          1..4953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    115..249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(152..171)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     206..225
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(308..327)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(592..609)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(763..1351)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(843..862)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1522..2313,2314..2382))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2145..2164
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2383..2487)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2542..2562)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             2877..2903
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(4937..4953)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4937..4953)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 taagtaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc tttaaaaaac
       61 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      121 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      181 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
      241 gtctggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
      301 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
      361 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
      421 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
      481 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgatatgc ggtgtgaaat
      541 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
      601 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      661 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      721 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      781 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      841 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      901 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      961 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1021 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1081 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1141 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1201 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1261 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1321 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1381 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1441 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1501 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1561 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1621 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     1681 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1741 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1801 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     1861 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1921 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1981 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2041 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2101 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     2161 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2221 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2281 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2341 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2401 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2461 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaccttct
     2521 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     2581 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaagg aaagtcccca
     2641 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     2701 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     2761 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     2821 ttccagaagt agtgaagagg cttttttgga ggagatctaa gcttgccgcc accatgtatg
     2881 atgttcctga ttatgctagc ctcccgggga attcggcaga ggcaccggct tccccggccc
     2941 cgctctctcc gctcgaagtg gagctggacc cggagttcga gccccagagc cgtccgcgat
     3001 cctgtacgtg gcccctgcaa aggccggagc tccaagcgag ccctgccaag ccctcggggg
     3061 agacggccgc tgactccatg atccccgagg aggaggacga tgaagacgac gaggacggcg
     3121 ggggacgggc cggctcggcc atggcgatcg gcggcggcgg cgggagcggc acgctgggct
     3181 ccgggctgct ccttgaggac tcggcccggg tgctggcacc cggagggcaa gaccccgggt
     3241 ctgggccagc caccgcggcg ggcgggctga gcgggggtac acaggcgctg ctgcagcctc
     3301 agcaaccgct gccaccgccg cagccggggg cggctggggg ctccgggcag ccgaggaaat
     3361 gttcgtcgcg gcggaacgcc tggggaaacc tgtcctacgc ggacctgatc acccgcgcca
     3421 tcgagagctc cccggacaaa cggctcactc tgtcccagat ctacgagtgg atggtgcgtt
     3481 gcgtgcccta cttcaaggat aagggcgaca gcaacagctc tgccggctgg aagaactcca
     3541 tccggcacaa cctgtcactg catagtcgat tcatgcgggt ccagaatgag ggaactggca
     3601 agagctcttg gtggatcatc aaccctgatg gggggaagag cggaaaagcc ccccggcggc
     3661 gggctgtctc catggacaat agcaacaagt ataccaagag ccgtggccgc gcagccaaga
     3721 agaaggcagc cctgcagaca gcccccgaat cagctgacga cagtccctcc cagctctcca
     3781 agtggcctgg cagccccacg tcacgcagca gtgatgagct ggatgcgtgg acggacttcc
     3841 gttcacgcac caattctaac gccagcacag tcagtggccg cctgtcgccc atcatggcaa
     3901 gcacagagtt ggatgaagtc caggacgatg atgcgcctct ctcgcccatg ctctacagca
     3961 gctcagccag cctgtcacct tcagtaagca agccgtgcac ggtggaactg ccacggctga
     4021 ctgatatggc aggcaccatg aatctgaatg atgggctgac tgaaaacctc atggacgacc
     4081 tgctggataa catcacgctc ccgccatccc agccatcgcc cactggggga ctcatgcagc
     4141 ggagctctag cttcccgtat accaccaagg gctcgggcct gggctcccca accagctcct
     4201 ttaacagcac ggtgttcgga ccttcatctc tgaactccct acgccagtct cccatgcaga
     4261 ccatccaaga gaacaagcca gctaccttct cttccatgtc acactatggt aaccagacac
     4321 tccaggacct gctcacttcg gactcactta gccacagcga tgtcatgatg acacagtcgg
     4381 accccttgat gtctcaggcc agcaccgctg tgtctgccca gaattcccgc cggaacgtga
     4441 tgcttcgcaa tgatccgatg atgtcctttg ctgcccagcc taaccaggga agtttggtca
     4501 atcagaactt gctccaccac cagcaccaaa cccagggcgc tcttggtggc agccgtgcct
     4561 tgtcgaattc tgtcagcaac atgggcttga gtgagtccag cagccttggg tcagccaaac
     4621 accagcagca gtctcctgtc agccagtcta tgcaaaccct ctcggactct ctctcaggct
     4681 cctccttgta ctcaactagt gcaaacctgc ccgtcatggg ccatgagaag ttccccagcg
     4741 acttggacct ggacatgttc aatgggagct tggaatgtga catggagtcc attatccgta
     4801 gtgaactcat ggatgctgat gggttggatt ttaactttga ttccctcatc tccacacaga
     4861 atgttgttgg tttgaacgtg gggaacttca ctggtgctaa gcaggcctca tctcagagct
     4921 gggtgccagg ctgaaggatc cactagttct aga
//
