LOCUS       sequence-33513-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Dittgen T, Nimmerjahn A, Komai S, Licznerski P, Waters J, Margrie 
            TW, Helmchen F, Denk W, Brecht M, Osten P
  TITLE     Lentivirus-based genetic manipulations of cortical neurons and their
            optical and electrophysiological monitoring in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Dec 28. 101(52):18206-11.
  PUBMED    15608064
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Dec 28. 101(52):18206-11."
COMMENT     Sequence Label: pFCK(0.4)GW
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    453..570
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(868..912)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccctgctacc atggtggcga ccggtacccg gggatcggct ctagagctgc ccccagaact
       61 aggggccact cgcctgcccg tgctcctgag tgcaaacgga gaaccggctt gactgacgag
      121 cttggggctt ctgagcaggg cactgtggct gctcacagcc tcgcgagcct tcgtcagcat
      181 ccaggtcccc atggcaacca cctccgaaac gccctgttcc cgttgccccg gtaactgcct
      241 ccccaacacc tgcctgcctt tcactttaaa acctgctcct ctttgccctg gccttatata
      301 cacaatactg caggactgtg tgggcccagg agcaagtgga ccctgttccc ccagggtgat
      361 gtactgaggg ggttggagaa ggggatgtga acaaacttag tccacaagtt aattaaccaa
      421 actggatctc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt aatttgtttt
      481 tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg
      541 taccccccaa tccccccttt tcttttaaaa ttgtggatga atactgccat ttgtctgcag
      601 aattggcgca cgcagtgccg atccgttcac taatcgaatg gatctgtctc tgtctctctc
      661 tccaccttct tcttctattc cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt
      721 ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa
      781 aactattctt aaacctacca agcctcctac tatcattatg aataatttta tataccacag
      841 ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc
      901 ttgttcattc ttttcttgct ggtttttcga ttcttcaatt aa
//
