LOCUS       sequence-335132-        9802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of RfxCas13d sgRNAs from a U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Li S, Li X, Xue W, Zhang L, Yang LZ, Cao SM, Lei YN, Liu CX, Guo SK,
            Shan L, Wu M, Tao X, Zhang JL, Gao X, Zhang J, Wei J, Li J, Yang L, 
            Chen LL
  TITLE     Screening for functional circular RNAs using the CRISPR-Cas13 
            system.
  JOURNAL   Nat Methods. 2021 Jan;18(1):51-59. doi: 10.1038/s41592-020-01011-4. 
            Epub 2020 Dec 7.
  PUBMED    33288960
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-01011-4"; journalName: "Nat Methods"; date: 
            "2021-01"; volume: "18"; issue: "1"; pages: "51-59"
COMMENT     Sequence Label: RfxCas13d-backbone
FEATURES             Location/Qualifiers
     source          1..9802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        251..280
                     /label=RfxCas13d DR30
                     /note="5' processed 30-base direct repeat for an RfxCas13d 
                     gRNA (Konermann et al., 2018)"
     CDS             637..942
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     821..840
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    1881..1998
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2137..3315
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2368..3306
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3263..3283
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3328..3927
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3328..3347)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3824..3844
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3943..4531
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3996..4016)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4414..4425)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4603..4836
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4914..5035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4951..4970)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5005..5024
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5075..5210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5230..5249)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5231..5249)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5259..5276)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5259..5275)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5268..5290)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5417..5872
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5504..5523)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5714..5735
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5898..6002
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6003..6071,6072..6863)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6221..6240)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7034..7622
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7523..7542
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7776..7793
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7910..7931
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7946..7951,7952..7969,7970..7976)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7984..8000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7989..8011
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8008..8024
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8008..8024
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8043..8063
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8045..8063
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8091..8317
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8318..8498
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8545..8670
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9163..9396
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9581..9625
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg aacccctacc aactggtcgg ggtttgaaac ggagacgacc ggtaccggtg
      301 tcttcgaggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
      361 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggtg tgctatgaag
      421 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
      481 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
      541 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
      601 acggctcttt tgctgacgag aacagggact ggtgaaatgc agtttaaggt ttacacctat
      661 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
      721 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
      781 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
      841 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
      901 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaaccctgca ggacgaagac
      961 ccgtttgaga gctaccatcg atgtagtctt caacattaac aactttaagt ccagcaattt
     1021 gagttaaggg tgttgctctc aatgatttca ttaatggttc aatttttaat ttcttttctt
     1081 ctggtttaaa attcaagttt aaagtgaaag tgtaatatgc acccatttct ttaaataaat
     1141 cttctaaata gtctactaat gttttatttt gttttttata aaatcaagca gcctctgcta
     1201 ttaatataga agcttgtatt ccatctttat ctctagctga gtcatcaatt acatatccat
     1261 aactttcttc ataagcaaaa acaaaattta atccgttatc ttcttcttta gcaatttctc
     1321 tacccattca tttaaatcca gttaaagttt ttacaatatt aactccatat ttttcatgag
     1381 cgattctatc acccaaatca cttgttacaa aacttgaata tagagccgga ttttttggaa
     1441 tgctatttaa gcgttttaga tttgataatt ttcaatcaat taaaattggt cctgtttgat
     1501 ttccatctaa tcttacaaaa tgaccatcat gttttattgc cattccaaat ctgtcagcat
     1561 ctgggtcatt cataataata atatctgcat catgtttaat accatattca agcggtattt
     1621 ttcatgcagg atcaaattct ggatttggat ttacaacatt tttaaatgtt tcatcttcaa
     1681 atgcatgctc ttcaacctca ataacgttat atcctgattc acgtaatatt tttggggtaa
     1741 atttagttcc tgttccatta actgcgctaa aaataatttt taaatctttt ttagcttctt
     1801 gctctttttt gtagaattcc gtctcttttt taagcttggc gtaactagat cttgagacaa
     1861 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     1921 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     1981 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     2041 ctcgaggggg cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc
     2101 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
     2161 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt
     2221 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     2281 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     2341 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
     2401 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     2461 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     2521 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     2581 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     2641 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     2701 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     2761 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     2821 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     2881 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     2941 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     3001 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     3061 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     3121 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     3181 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     3241 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     3301 tttcaggtgt cgtgacgtac ggccaccatg accgagtaca agcccacggt gcgcctcgcc
     3361 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     3421 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     3481 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     3541 gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     3601 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     3661 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggagtctcg
     3721 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     3781 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     3841 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     3901 tgcatgaccc gcaagcccgg tgcctgaacg cgttaagtcg acaatcaacc tctggattac
     3961 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4021 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4081 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4141 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4201 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4261 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4321 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4381 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4441 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4501 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     4561 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4621 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4681 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4741 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4801 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     4861 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     4921 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4981 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5041 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5101 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5161 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     5221 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     5281 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5341 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5401 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5461 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5521 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5581 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5641 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5701 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5761 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5821 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     5881 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5941 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6001 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6061 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6121 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6181 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6241 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6301 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6361 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6421 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6481 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6541 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6601 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6661 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6721 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6781 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6841 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6901 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6961 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7021 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7081 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7141 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7201 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7261 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7321 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7381 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7441 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7501 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7561 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7621 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7681 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7741 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7801 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7861 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     7921 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     7981 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     8041 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     8101 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     8161 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     8221 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     8281 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     8341 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8401 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8461 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     8521 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     8581 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     8641 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     8701 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     8761 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     8821 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     8881 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     8941 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9001 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     9061 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     9121 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     9181 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     9241 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     9301 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     9361 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     9421 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     9481 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     9541 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     9601 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     9661 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     9721 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     9781 cccaaccccg aggggaccca ga
//
