LOCUS       sequence-335160-        9040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human CDR1as/ciRS-7 circRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Hansen TB, Jensen TI, Clausen BH, Bramsen JB, Finsen B, Damgaard CK,
            Kjems J
  TITLE     Natural RNA circles function as efficient microRNA sponges.
  JOURNAL   Nature. 2013 Mar 21;495(7441):384-8. doi: 10.1038/nature11993. Epub 
            2013 Feb 27.
  PUBMED    23446346
REFERENCE   2  (bases 1 to 9040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11993"; journalName: "Nature"; date: "2013-03-21- 
            21"; volume: "495"; issue: "7441"; pages: "384-8"
COMMENT     Sequence Label: pcDNA3 - ciRS-7
FEATURES             Location/Qualifiers
     source          1..9040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4593..4611)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4594..4611)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4631..4648)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4637..4861
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4907..5335
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4994..5013)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5204..5225
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5344..5364)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5349..5678
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5529..5664
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5591..5610
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5745..6539
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5799..5818)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6409..6428
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6713..6834
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6750..6769)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6804..6823
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6883..6899)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6883..6899)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6896..6918)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6907..6923
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6931..6937,6938..6955,6956..6961))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6976..6997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7114..7131)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7285..7873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7365..7384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8044..8835,8836..8904))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8667..8686
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8905..9009)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttctgtaa
      901 gagtagtctc atgatgtctt ttaatttaat atctaggccc taatacacaa ctctttgaat
      961 attgctacta tctttcagca tgactttcca ataccattaa gattaagcct ttctgttctt
     1021 cagtttctct aactcaagtc ttctaagctc cacagcttcc aacatcttta attaccagat
     1081 cttcccaata ttataacatc cgtgcattcc agtccctgtc ctgcagcatc tccaatgccc
     1141 ttgttttcta aacttcccag tattcaagtc attaaaaatt taggtcttct aatatctcca
     1201 acaacccatc ttcaaagtcc aggtcttgtc tttcagaatc cagagttcta aattacaagt
     1261 cttccatggt cctaatcctc caatgtctcc tatatacagg tcttctagag gctatgtatt
     1321 tcatagtgtt taagtctaga ttcttcaatg tcttccagca cttacatcat tcagttttta
     1381 catttttaaa tgtctcctac tttctagttt tacagtgtat ttttatcgcc aattcctcca
     1441 atgcccaagt ctttaaatac ctgcaagaac tttcaggtac atcaatatcc tcaatgtctg
     1501 aatgaataga cctcttaata tcctggtctc atattgtcca ggctgccagt attcttgaca
     1561 tccagcatct ttaaaattta attcttccaa actgtctttt tgacataaat atgtcaatat
     1621 ccatgcttac caatgtctcc caatatccag ggcttctcat tttaggattt ataaatgtct
     1681 acatctgctg ttgtttatat ctgccagtat ttaggagttc caaggtacag gacttacaat
     1741 atctctaatt tccagagatt tcagtgtcta tgacttccaa tattcaatat cccaatgttc
     1801 agggcctcca gaaatatcaa tgtccagagt aaccaatgtc ttcaaaatcc aaggcttgca
     1861 atgtctccaa tatcctgtgc ttacaatgtt tccaatgtcc agggtttccg atggcacctg
     1921 tgtcaaggtc ttccaacaac tccgggtctt ccagcgactt caagtcttcc aataatctca
     1981 aggtcttcca gataatcctg agcttccaga aaatccacat cttccagaca atccatgtct
     2041 tccggacaat ccatgtcttc caagaagctc caagtcttcc agtaaatcaa gtcttccagc
     2101 aaatccagtc ttccagcaat tactggtctt ccaccaaatc cagatcttcc aggaaaatcc
     2161 acgtcttcca ggaaatccat gtcttccaat aatttcaagg tcttccatca aatacagatc
     2221 ttccagctaa tccatgtctt ccagaaaaat ctgtgtcttc caccaaatcc aagtcttcca
     2281 gtaaatctag ttcttccaga aaaatctaga tcttccagtc aatcagtgtc ttccagaaag
     2341 aaatccaggt cttccagtca atcagtgtct tccagaaaga aatccaggtc ttccagtcag
     2401 tcagtgtctt ccagaaaaat ctacgtcttc caccaaatcc aggtcttcca gtcaatccac
     2461 atcttccgga aaaaatccag gtcttccagc caatatatgt cttcctgaag atccacgtct
     2521 tccagaaaat ccatgtcttc cagaaaatcc atgtcttcca gtaacctccc agtcttccag
     2581 aaaatccacg tcttcccaac aatccaagtc ttccggataa tttgggtctt cctgaaaatc
     2641 tacgtcttcc aaaaaagcca tgtcttccag aaaatccaca tcttccaatg gcctccaggt
     2701 cttccagact atccatgtct tccagaaaat ccttgtcttc ccttaaatct atagcttcca
     2761 aaaaatccgg gtcttccagg aaatccgtgt cttccagcaa gtccacgtct tccaacaaag
     2821 ccatgtcttc cagactatcc atgtcttcca gaaaatcctt gtcttccctc aaatccatag
     2881 cttccgaaaa atccaggtct tccaggaaat ccgtgtcttc cagcaaatcc acgtcttcca
     2941 acaaagccat gtcttccatc aaattaatgt cttccagcct acttgtgtct tccaacaaag
     3001 gtacgtcttc caacaaaggt acgtcttcca acaaaggtat gtcttccaac aaaggtacgt
     3061 cttccagaaa atccacgtct tccaaccaag ccatgtcttc cagaaaatcc acgtcttcca
     3121 gaaaatatat gtcttccaac taagctacgt cttccaacaa atccatgtct tcctatatct
     3181 ccaggtcttc cagcatctcc agggcttcca gcatctgctc gtcttccaac atctccacgt
     3241 cttccagcat ctctgtgtct tccagcatct tcatgtcttc caacaactac ccagtcttcc
     3301 atcaactggc tcaatatcca tgtcttccaa cgtctccagt gtgctgatct tctgacattc
     3361 aggtcttcca gtgtctgcaa tatccaggta ttctaacatg ttctatagtc cagatcttcc
     3421 agcctttccc caggtccagg ccttttcaaa tctaggcttc ccctgtctga gccttcccat
     3481 gttaaaatca ctctacagaa aaatcttcca actcaactat ttctgcttct agttgaatta
     3541 gcttcaaaaa catttaatgg gacattcctc cttatagacc agatcttatg aaccaacagt
     3601 actgcagttt ggcggccgct cgactggtta ctctggacat tgatatttct ggaggccctg
     3661 aacattggga tattgaatat tggaagtcat agacactgaa atctctggaa attagagata
     3721 ttgtaagtcc tgtaccttgg aactcctaaa tactggcaga tataaacaac agcagatgta
     3781 gacatttata aatcctaaaa tgagaagccc tggatattgg gagacattgg taagcatgga
     3841 tattgacata tttatgtcaa aaagacagtt tggaagaatt aaattttaaa gatgctggat
     3901 gtcaagaata ctggcagcct ggacaatatg agaccaggat attaagaggt ctattcattc
     3961 agacattgag gatattgatg tacctgaaag ttcttgcagg tatttaaaga cttgggcatt
     4021 ggaggaattg gcgataaaaa tacactgtaa aactagaaag taggagacat ttaaaaatgt
     4081 aaaaactgaa tgatgtaagt gctggaagac attgaagaat ctagacttaa acactatgaa
     4141 atacatagcc tctagaagac ctgtatatag gagacattgg aggattagga ccatggaaga
     4201 cttgtaattt agaactctgg attctgaaag acaagacctg gactttgaag atgggttgtt
     4261 ggagatatta gaagacctaa atttttaatg acttgaatac tgggaagttt agaaaacaag
     4321 ggcattggag atgctgcagg acagggactg gaatgcacgg atgttataat attgggaaga
     4381 tctggtaatt aaagatgttg gaagctgtgg agcttagaag acttgagtta gagaaactga
     4441 agaacagaaa ggcttaatct taatggtatt ggaaagtcat gctgaaagat agtagcaata
     4501 ttcaaagagt tgtgtattag ggcctagata ttaaattaaa agacatcatg agactactct
     4561 tacaggtcga gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct
     4621 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     4681 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4741 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4801 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     4861 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     4921 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4981 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5041 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5101 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5161 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5221 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5281 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     5341 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5401 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5461 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5521 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5581 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     5641 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     5701 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     5761 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     5821 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     5881 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     5941 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     6001 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     6061 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     6121 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     6181 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     6241 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     6301 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     6361 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     6421 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     6481 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     6541 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     6601 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6661 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     6721 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6781 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6841 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6901 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6961 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7021 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7081 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7141 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7201 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7261 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7321 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7381 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7621 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7681 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7741 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7801 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7861 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7921 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7981 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8041 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8101 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8161 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8221 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8281 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8341 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8401 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8461 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8521 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8581 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8641 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8701 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8761 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8821 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8881 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8941 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9001 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
