LOCUS       sequence-33517-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Barkan D, Rao V, Sukenick GD, Glickman MS
  TITLE     Redundant function of cmaA2 and mmaA2 in Mycobacterium tuberculosis 
            cis cyclopropanation of oxygenated mycolates.
  JOURNAL   J Bacteriol. 2010 Jul . 192(14):3661-8.
  PUBMED    20472794
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2010 Jul . 192(14):3661-8."
COMMENT     Sequence Label: pMSG360zeo
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(49..54,55..72,73..79)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    85..104
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             151..525
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     protein_bind    577..610
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttcgccgggc ctcggcgacc gccgcgtcga ggcctccaga ccccaggctt gacactttat
       61 gcttccggct cgtataatgt gtggaattgt gagcgctcac aattcgcatg ctgataaatg
      121 cttcaataat agcacgtgag gagggccacc atggccaagt tgaccagtgc cgttccggtg
      181 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
      241 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
      301 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
      361 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
      421 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
      481 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gcctcgagat
      541 aaaattgcag gaattcgatg cctgacgccc cagcttataa cttcgtataa tgtatgctat
      601 acgaagttat tcgaactgta ccggatttcg gacaggactc tagaggatcc ccgggtacct
      661 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
      721 acttatgact gtcttcttta tcatgcaac
//
