LOCUS       sequence-335213-        2086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Part 1a of HexaPro-D614G spike to be used in the Spike 
            Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 1a Spacer
FEATURES             Location/Qualifiers
     source          1..2086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(451..485)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(556..1215)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1130..1149
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1397..1985
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1886..1905
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggcacctg ccacctgaca tgttcgtgtt cctggtgctc ctgcctctgg tgagcagcca
       61 gtgcgtgaac ctgaccaccc gaacccagct cccaccagcc tacaccaaca gctttacacg
      121 gggcgtgtac taccctgaca aggtgttcag atctagcgtc ctgcacagca ctcaggacct
      181 cttcctgccg ttcttcagca acgtgacatg gttccacgcc atccacgtga gcggcacaaa
      241 cggaaccaag cggtttgata accccgtcct gccattcaat gatggagttt acttcgccag
      301 taccgagaag agtaacatca tccggggctg gatcttcggc accaccctgg atagcaaaac
      361 acagagcctc ctgatcgtga acaatgccac gaacgtcgtg atcaaggtgt gcgagttcca
      421 gttttgcaag gtggcaggtg gaccagacca ataaaaaacg cccggcggca accgagcgtt
      481 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
      541 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
      601 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
      661 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
      721 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
      781 aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
      841 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
      901 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
      961 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     1021 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     1081 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     1141 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     1201 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     1261 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa
     1321 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1381 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1441 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1501 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1561 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1621 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1681 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1741 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1801 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1861 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1921 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1981 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2041 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtag
//
