LOCUS       sequence-335214-        2067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Part 1b of HexaPro-D614G spike to be used in the Spike 
            Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 1b Spacer
FEATURES             Location/Qualifiers
     source          1..2067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(432..466)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(537..1196)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1111..1130
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1378..1966
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1867..1886
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggcacctg ccaccgcaat gatcctttcc tgggtgtgta ctaccacaag aacaacaaga
       61 gctggatgga aagcgagttc agagtctaca gcagcgccaa caactgcaca ttcgagtacg
      121 tctctcagcc ttttctgatg gaccttgagg ggaaacaagg caacttcaag aacctgagag
      181 aattcgtgtt caagaacatc gacggctact tcaaaatcta ctccaagcac acacccatca
      241 acctggtccg ggacctccct cagggcttca gcgccctgga acccctggtc gacctgccca
      301 taggcatcaa cataacgcgg ttccaaaccc tgctggccct gcatagatcc tacctgactc
      361 ctggcgacag cagcagcgga tggaccgccg gagctgcagc ctactatgtg ggtggcaggt
      421 ggaccagacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
      481 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
      541 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
      601 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
      661 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
      721 tttaaatcaa aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca
      781 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
      841 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
      901 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
      961 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     1021 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     1081 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     1141 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     1201 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     1261 tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta
     1321 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1381 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1441 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1501 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1561 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1621 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1681 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1741 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     1801 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1861 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     1921 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     1981 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2041 atcccctgat tctgtggata accgtag
//
