LOCUS       sequence-335215-        2477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Part 1 of HexaPro-D614G spike to be used in the Spike 
            Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 1 Spacer
FEATURES             Location/Qualifiers
     source          1..2477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(842..876)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(947..1606)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1521..1540
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1788..2376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2277..2296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggcacctg ccacctgaca tgttcgtgtt cctggtgctc ctgcctctgg tgagcagcca
       61 gtgcgtgaac ctgaccaccc gaacccagct cccaccagcc tacaccaaca gctttacacg
      121 gggcgtgtac taccctgaca aggtgttcag atctagcgtc ctgcacagca ctcaggacct
      181 cttcctgccg ttcttcagca acgtgacatg gttccacgcc atccacgtga gcggcacaaa
      241 cggaaccaag cggtttgata accccgtcct gccattcaat gatggagttt acttcgccag
      301 taccgagaag agtaacatca tccggggctg gatcttcggc accaccctgg atagcaaaac
      361 acagagcctc ctgatcgtga acaatgccac gaacgtcgtg atcaaggtgt gcgagttcca
      421 gttttgcaat gatcctttcc tgggtgtgta ctaccacaag aacaacaaga gctggatgga
      481 aagcgagttc agagtctaca gcagcgccaa caactgcaca ttcgagtacg tctctcagcc
      541 ttttctgatg gaccttgagg ggaaacaagg caacttcaag aacctgagag aattcgtgtt
      601 caagaacatc gacggctact tcaaaatcta ctccaagcac acacccatca acctggtccg
      661 ggacctccct cagggcttca gcgccctgga acccctggtc gacctgccca taggcatcaa
      721 cataacgcgg ttccaaaccc tgctggccct gcatagatcc tacctgactc ctggcgacag
      781 cagcagcgga tggaccgccg gagctgcagc ctactatgtg ggtggcaggt ggaccagacc
      841 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
      901 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
      961 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     1021 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     1081 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     1141 aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt
     1201 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     1261 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     1321 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     1381 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     1441 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     1501 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     1561 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     1621 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     1681 gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt
     1741 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1801 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1861 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1921 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1981 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2041 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2101 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2161 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2221 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2281 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2341 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2401 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2461 tctgtggata accgtag
//
