LOCUS       sequence-335216-        2442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Part 2 of HexaPro-D614G spike to be used in the Spike 
            Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 2 Spacer
FEATURES             Location/Qualifiers
     source          1..2442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(807..841)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(912..1571)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1486..1505
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1753..2341
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2242..2261
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggcacctg ccacctgtgg gctacctgca acctagaacc ttcctgctga agtacaacga
       61 gaacggcaca atcacagacg ccgtcgactg cgccctggac cctctctctg agacaaagtg
      121 caccctgaag tccttcaccg tggaaaaggg catctaccag accagcaact tccgggtgca
      181 gcctacagag agcatcgtgc gatttccaaa cattaccaac ctctgcccct tcggcgaggt
      241 gtttaacgcc acaagatttg cctccgttta cgcctggaat agaaagagaa tcagcaattg
      301 tgtggccgac tactccgtgc tgtataacag cgcctctttc agcaccttca agtgctacgg
      361 cgtttcccca acaaagctga atgacctgtg cttcaccaac gtgtacgccg actccttcgt
      421 aattagaggc gatgaggtgc ggcagatcgc accaggccag accggtaaga tcgctgacta
      481 caactataag ctgcctgatg attttacagg ctgcgtgatc gcctggaact ctaacaacct
      541 ggatagcaag gtgggcggca actacaacta cctgtaccgg ctgtttcgca agtctaacct
      601 gaaacctttc gagagagaca tctccacaga gatctaccag gccggttcta caccttgtaa
      661 cggggtggaa ggcttcaact gttacttccc tctgcaaagc tacggcttcc agcctaccaa
      721 tggagtcggc taccagccat accgggtggt cgtgctgtcc ttcgagttac tccacgcccc
      781 cgccaggtgg caggtggacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga
      841 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
      901 tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
      961 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     1021 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     1081 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg
     1141 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     1201 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     1261 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     1321 atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg
     1381 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     1441 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     1501 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     1561 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     1621 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat
     1681 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1741 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1801 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1861 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1921 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1981 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2041 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2101 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2161 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2221 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2281 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2341 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2401 gttctttcct gcgttatccc ctgattctgt ggataaccgt ag
//
