LOCUS       sequence-335217-        2473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Part 3 of HexaPro-D614G spike to be used in the Spike 
            Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 3 Spacer
FEATURES             Location/Qualifiers
     source          1..2473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(838..872)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(943..1602)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1517..1536
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1784..2372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2273..2292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggcacctg ccaccgccac cgtctgcggt cctaagaagt ccaccaatct ggttaagaac
       61 aaatgcgtga acttcaactt caacggcctg accgggaccg gcgtgctgac cgaaagcaac
      121 aaaaagttcc tccccttcca gcagttcggc cgtgatatcg ctgacaccac agatgccgtc
      181 agagatccac agaccctgga aatcctggat attacaccct gctccttcgg aggagtttct
      241 gtgatcaccc ccgggaccaa taccagcaac caggtggctg tgctgtacca aggtgttaac
      301 tgcaccgagg ttcctgtggc catccacgcc gatcagctga cacctacttg gagagtgtac
      361 tccactggct ccaatgtgtt ccagaccagg gccggatgtc tgatcggcgc cgagcacgtg
      421 aataacagtt acgagtgcga catccctatc ggcgccggca tctgtgccag ctaccagacc
      481 cagacaaaca gccctgggtc tgcttcctct gtagctagcc agagcatcat cgcctacacc
      541 atgagcctgg gcgcagagaa cagcgtggcc tattccaaca actctatcgc cattcccacc
      601 aactttacaa ttagcgtcac aacagagatc ctgcccgtga gcatgaccaa gaccagcgtg
      661 gactgtacaa tgtacatctg tggcgacagc actgaatgca gcaacctgct gctgcaatac
      721 ggctcctttt gcacccaact gaaccgggcg ctgaccggaa tcgccgtgga acaggacaaa
      781 aatacccagg aggtgttcgc ccaagtgaag cagatcggtg gcaggtggac cagaccaata
      841 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
      901 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
      961 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     1021 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     1081 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     1141 ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg
     1201 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     1261 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     1321 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     1381 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     1441 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     1501 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     1561 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     1621 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     1681 gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt gagttttcgt
     1741 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1801 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1861 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1921 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1981 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2041 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2101 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2161 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2221 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2281 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2341 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2401 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2461 tggataaccg tag
//
