LOCUS       sequence-335218-        2475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Part 4 of HexaPro-D614G spike to be used in the Spike 
            Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 4 Spacer
FEATURES             Location/Qualifiers
     source          1..2475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(840..874)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(945..1604)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1519..1538
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1786..2374
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2275..2294
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggcacctg ccaccgatct acaagacccc acctatcaag gacttcggcg gctttaactt
       61 tagccagatt ctccctgatc cttctaagcc tagcaagcgg agccctatcg aggatctgct
      121 gttcaacaag gtcaccctgg ccgatgccgg ctttatcaaa cagtatggcg attgcctggg
      181 cgacatagcc gccagagatc tgatctgcgc ccagaaattc aacggcctga cagttctccc
      241 acctctgctg accgacgaga tgatcgctca gtacacctct gccctgctgg ctggcaccat
      301 cacatctggg tggacatttg gcgccggccc cgccctgcag atcccctttc ccatgcagat
      361 ggcctataga ttcaacggaa tcggcgtgac ccagaacgtg ctgtatgaaa accagaagct
      421 gatcgctaac cagttcaatt ctgccatcgg caagatccag gactccctct cctctacccc
      481 cagcgccctg ggcaaactgc aggacgtggt gaatcagaac gcccaagccc tgaacaccct
      541 ggtgaagcag ctcagcagca attttggcgc catcagctct gtgctgaacg atatcctgtc
      601 tagactggac cctccagaag ccgaagtcca gatcgataga ctgatcacag gcagactgca
      661 gtccctgcaa acctacgtga cccaacagct gatcagggcc gctgaaataa gagccagcgc
      721 caatctcgcc gctaccaaga tgtccgagtg tgtgctggga cagtctaaac gcgttgactt
      781 ctgcggcaaa ggctatcacc tgatgagctt cccccagagg tggcaggtgg accagaccaa
      841 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      901 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
      961 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     1021 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     1081 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     1141 ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta
     1201 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     1261 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     1321 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     1381 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     1441 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     1501 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     1561 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     1621 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     1681 aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc
     1741 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1801 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1861 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1921 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1981 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2041 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2101 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2161 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2221 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2281 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2341 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2401 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2461 tgtggataac cgtag
//
