LOCUS       sequence-335219-        2137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Part 5 of HexaPro-D614G spike to be used in the Spike 
            Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 5 Spacer
FEATURES             Location/Qualifiers
     source          1..2137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(502..536)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(607..1266)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1181..1200
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1448..2036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1937..1956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggcacctg ccacccagag cgcgccgcac ggcgtggtgt tcctgcatgt gacatacgtg
       61 cctgcccaag agaagaattt cacaaccgcc cctgccatct gccacgacgg caaggcccac
      121 ttcccaagag agggcgtttt cgtttccaat ggcacacact ggttcgtgac acaaagaaac
      181 ttctacgaac cccagattat caccaccgac aacaccttcg tgagtggcaa ttgtgacgtg
      241 gtcatcggaa tcgtgaacaa cacagtgtac gaccctctgc aacctgagct ggactctttt
      301 aaggaagagc tggacaagta ctttaaaaac cacaccagcc ccgatgtgga cctgggcgac
      361 atcagtggca ttaacgccag cgtggtgaac atccaaaagg aaatcgacag actgaacgag
      421 gtggccaaga acctgaacga gtccctgatc gacctgcagg agctcggcaa atacgagcag
      481 ggtggcaggt ggaccagacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
      541 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
      601 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
      661 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
      721 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
      781 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg aaacgaaaaa
      841 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
      901 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
      961 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1021 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     1081 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1141 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     1201 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     1261 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     1321 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata accgtag
//
