LOCUS       sequence-335228-        3518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0-CDS1 promoterless vector encoding 
            Neonothopanus nambi hispidin-3-hydroxylase nnH3H codon-optimised for
            expression in Nicotiana benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS
  TITLE     Plants with genetically encoded autoluminescence.
  JOURNAL   Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9.
  PUBMED    32341562
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9."
COMMENT     Sequence Label: pX019
FEATURES             Location/Qualifiers
     source          1..3518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1316..1334)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1402..1506
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1728..2519
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2690..3278
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3179..3198
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3432..3449
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcaaat ggcctctttt gaaaattcac tctctgtgtt gatcgtggga gccggtttgg
       61 gtggtctagc agctgctatc gctctccgac gacagggcca cgtggttaag atctacgact
      121 catctagctt taaggccgag ctcggcgcag gcttggcagt tccaccgaat acactgcgta
      181 gtctacagca attgggttgt aacactgaaa accttaacgg cgttgataat ctctgtttta
      241 ctgccatggg atatgatggg tcagtgggca tgatgaacaa tatgactgat taccgtgaag
      301 catatggcac tagttggatc atggttcatc gtgtcgattt gcataacgag ttgatgagag
      361 ttgctttgga cccaggggga ttgggccctc ccgcaacgct tcacctgaat catcgagtca
      421 cgttttgtga tgtagatgct tgtacggtga catttaccaa cggtacaact caaagtgcag
      481 atcttatcgt tggggctgac gggatacgtt caactatccg tagatttgtc ttggaggagg
      541 acgtgaccgt tcccgcaagc ggaattgtgg ggtttcgatg gttggtacag gctgatgccc
      601 tcgatccata tcctgaactc gattggatag tcaaaaagcc accgctcgga gctaggttga
      661 tttctacgcc ccagaatcca caatctggag tgggactggc agatcgtaga actattatta
      721 tatacgcttg tcgtgggggg acaatggtaa atgtgctggc cgtacacgat gacgagcgag
      781 atcagaatac tgcagattgg agcgttccag catcaaagga tgacttgttt cgagtattcc
      841 atgactacca tccgagattc agacggctct tggaattggc tcaagatata aatctctggc
      901 agatgagggt tgtgccggtg ctcaagaaat gggtgaacaa acgagtgtgc ctgcttggag
      961 atgccgctca tgcctctctt ccgactctcg gccagggttt cgggatggga ttggaggacg
     1021 ccgtagccct tggtactctc ttgccaaaag gtacgactgc tagtcaaata gagacaagac
     1081 tggctgtcta cgaacagctt agaaaggacc gagcagagtt tgtagccgct gaaagttatg
     1141 aagagcagta tgtccccgag atgaggggct tgtacttgcg tagcaaggaa cttagagata
     1201 gagtcatggg gtatgacata aaagtggagt ctgagaaggt actggaaacc ttgcttagga
     1261 gctctaactc agcttagctt tgagaccacg aagtggctct tcagtggacg aaagggcctc
     1321 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     1381 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     1441 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1501 aagagtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     1561 gcagtggcgg ttttcatggc ttgttatgac tgtttttttt tggggtacag tctatgcctc
     1621 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     1681 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg
     1741 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     1801 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     1861 acagcgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     1921 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     1981 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     2041 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     2101 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     2161 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     2221 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     2281 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     2341 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     2401 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     2461 agttggaaga atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac
     2521 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2581 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2641 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2701 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2761 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2821 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2881 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2941 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3001 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3061 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3121 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3181 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3241 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3301 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3361 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3421 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3481 ctctccccgc gcgttggccg attcattaat cactctgt
//
