LOCUS       sequence-335229-        3170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0-CDS1 promoterless vector encoding 
            Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for 
            expression in Nicotiana benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS
  TITLE     Plants with genetically encoded autoluminescence.
  JOURNAL   Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9.
  PUBMED    32341562
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9."
COMMENT     Sequence Label: pX020
FEATURES             Location/Qualifiers
     source          1..3170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(968..986)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1054..1158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1380..2171
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2342..2930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2831..2850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3084..3101
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcaaat ggcacctatt tcatccacgt ggagcaggtt gataaggttc gtggctgtag
       61 aaacttccct ggttcatatt ggtgagccaa ttgatgcaac gatggacgtt ggcctcgccc
      121 gtagagaagg taagacaatt caagcatacg aaataatcgg aagcggcagt gccctcgatc
      181 tttctgcaca agtctctaag aacgtactca cagttaggga attgttgatg ccgctcagtc
      241 gagaagagat taagaccgtt cgatgcctcg gcttgaacta tccggtccac gccacagaag
      301 ctaacgtggc cgtgccgaag tttcctaacc tgttctataa gcccgtaaca tcattgattg
      361 gtccaggcgg tcttattact attccctccg tcgttcaacc ccccaaggag caccagtcag
      421 attacgaggc tgagctggtt atagtaatag gtaaggcagc aaagaacgta tcagaggatg
      481 aggctttgga ttatgtattg ggctataccg ctgcaaacga tatatctttc cgtaagcatc
      541 aattggctgt cagtcaatgg agcttcagca agggctttga tggaactaat ccacttggtc
      601 cctgcttggt ttccgcctcc agtatccctg acccacagga tattccaata cagtgcaaat
      661 tgaatggtgg agtcgtccaa aatggcaaca ccagagatca aatcttcaac gttaagaaaa
      721 ccatatcatt tctttcccag gggactactt tggagcccgg ttccattatc cttaccggga
      781 ccccagatgg ggtggggttc gtacgaaacc cgcccctgta tttgaaggat ggcgatgagg
      841 ttatgacttg gatcggtagt ggaatcggaa ctctcgcaaa tacagttcga gaagaacaaa
      901 cgtgctttgc atcaggcggc cacgaatagc tttgagacca cgaagtggct cttcagtgga
      961 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     1021 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     1081 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     1141 tattgaaaaa ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     1201 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac
     1261 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     1321 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     1381 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     1441 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     1501 gcctgaagcc acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     1561 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     1621 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     1681 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     1741 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     1801 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     1861 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     1921 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     1981 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     2041 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     2101 cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag
     2161 tcggcaaata actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2221 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2281 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2341 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2401 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2461 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2521 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2581 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2641 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2701 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2761 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2821 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2881 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2941 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3001 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3061 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3121 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atcactctgt
//
