LOCUS       sequence-335230-        4688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0-SP promoterless vector encoding N-terminal 
            half of the Neonothopanus nambi hispidin synthase nnHispS 
            codon-optimised for expression in Nicotiana benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS
  TITLE     Plants with genetically encoded autoluminescence.
  JOURNAL   Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9.
  PUBMED    32341562
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9."
COMMENT     Sequence Label: pX021
FEATURES             Location/Qualifiers
     source          1..4688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     69..88
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     322..339
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2894..2912)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2980..3084
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3306..4097
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4268..4688,1..168)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
       61 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      121 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      181 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      241 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      301 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
      361 cgcaaaccgc ctctccccgc gcgttggccg attcattaat cactctgtgg tctcaaatga
      421 actcaagtaa gaatccgccc tccacattgt tggatgtatt tctcgacacg gctagaaatc
      481 ttgataccgc tagtaggaat gtcctggaat gcggggaaca tcgttggtca tatcgtgaat
      541 tggatacggt aagctctgcc ctggcccagc accttcgata taccgtcggc ttgagtccta
      601 cagtagccgt gatcagtgag aaccaccctt acatattggc cttgatgttg gccgtttgga
      661 aattgggggg taccttcgct ccaatcgacg tacatagtcc cgccgagctc gtggctggca
      721 tgttgaatat agttagcccc agctgtctgg ttataccttc tagtgacgtt accaaccaga
      781 cattggcctg cgatctcaat ataccagtag tagctttcca ccctcatcaa tcaacgatac
      841 cggaactgaa caaaaagtac ttgactgaca gtcaaatatc acctgacttg cctttccccg
      901 accccaacag gccggctctt tatctgttta ccagcagcgc tacttctcgt tctaatctga
      961 aatgcgtccc cttgacgcat acatttatcc ttagaaactc tctttcaaaa agggcctggt
     1021 gcaaacgtat gaggcccgaa accgattttg atggtattcg tgtactcggt tgggctcctt
     1081 ggtcccacgt gcttgcacat atgcaggaca ttggaccatt gacgcttttg aacgcagggt
     1141 gctacgtttt tgctacgact ccttcaacat atccgacaga gctgaaggac gatcgagatg
     1201 tgatctcctg tgccgccaac gcagtcatgt ataagggagt taagtcattt gcctgtctac
     1261 cttttgtgtt gggcggactt aaagcactct gcgaaagcga gccatctgta aaagctcagt
     1321 tgcaagtgga agagcgagca caattgctca aatccctgca gcatatgaag atcctggagt
     1381 gcggtggtgc catgttggaa gtctccgttg catcctgggc aatagagaac agaatcccca
     1441 taagcattgg gataggcatg actgaaactg gtggagccct cttcgctgga ccagttcagg
     1501 caatacaaac aggattttca tcagaggaca agttcataga ggacgccact tatcttttgg
     1561 tgaaggatga ctatgagtcc cacgccgagg aagatattaa cgaaggtgaa ctcgtcgtca
     1621 agtccaggat gctgcctcgt gggtatctgg ggtataatga tccatcattc agcgttgatg
     1681 atgcaggctg ggtgacgttc aaaacgggag ataggtacag tgttacaccc gacggcaaat
     1741 ttagctggct ggggagaaat accgacttca ttcagatgac atctggtgaa accttggacc
     1801 ctcgtccgat agaaagtctc ctctgtgagt cctcacttat ttctagggcc tgcgtaattg
     1861 gcgataagtt tcttaacggg ccagcaactg ctgtttgtgc cataatcgaa ctggaaccaa
     1921 ccaccgttga gaagggtcaa gctcattcca gagacatagc taggatattc gcccccatca
     1981 acagggattt gcccccccct ttgcgtatcg catggtcaca tgtcctggtt ttgcagccta
     2041 gtgagaagat acctatgaca aagaagggga cgatttttcg aaaaaagatc gaacaagtct
     2101 ttgggtccgc attgggtgga tcatccggcg acaatagcca agctactact gacgctagcg
     2161 tggtaagacg tgacgaactc agtaacacag tgaagcacat tatatcccga gtgttggggg
     2221 tgtctgatga cgaactcttg tggaccctct ctttcgccga gttgggtatg acttctgctt
     2281 tggccaccag gattgcaaat gagttgaacg aagtccttgt gggagtgaac ttgcctataa
     2341 acgcctgcta tattcatgtg gatcttccga gcctcagtaa tgcagtctat gccaaactcg
     2401 cacacttgaa gttgccagac cgtactccgg aaccacgaaa ggcaccagtt gaaaatcccg
     2461 gaggtaaaga gatcgtcatt gtaggccagg ccttccgttt gcccgggagt atcaacgacg
     2521 tcgcctctct tagggatgcc tttttggcta ggcaggccag ctcaattatt accgagatac
     2581 ctccggacag gtgggaccac gcatccttct acccaaaaga tattcgtttc aataaggctg
     2641 ggctggtgga catcgccaac tacgatcatt ccttctttgg gctgaccgcc acggaagcac
     2701 tctatctcag ccctacgatg cgactcgcct tggaggtgtc atttgaagca ttggagaatg
     2761 ccaacattcc agtttctcaa ctgaagggct cccagacagc agtgtatgtt gcaacaacag
     2821 acgatggttt tgaaacgttg ctcaacgccg aagcaggttg agaccacgaa gtggctcttc
     2881 agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     2941 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3061 caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     3121 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttttg
     3181 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     3241 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     3301 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     3361 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     3421 atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc gtaaggcttg
     3481 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     3541 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     3601 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     3661 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     3721 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     3781 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     3841 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     3901 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     3961 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     4021 tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc gagatcacca
     4081 aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag attgatttaa
     4141 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4201 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4381 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4621 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4681 ccgaaggg
//
