LOCUS       sequence-335231-        4907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0-CDS2 promoterless vector encoding 
            C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS
            codon-optimised for expression in Nicotiana benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS
  TITLE     Plants with genetically encoded autoluminescence.
  JOURNAL   Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9.
  PUBMED    32341562
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9."
COMMENT     Sequence Label: pX022
FEATURES             Location/Qualifiers
     source          1..4907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      223..811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     712..731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     965..982
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3756..3774)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3842..3946
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4168..4907,1..52)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga
       61 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
      121 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
      181 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
      241 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      301 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      361 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
      421 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
      481 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      541 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
      601 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
      661 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      721 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      781 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      841 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
      901 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
      961 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     1021 cgcgcgttgg ccgattcatt aatcactctg tggtctcaag gttacgatgc ctacaccaga
     1081 ttttacggga ctgggagggc tgcctctacg gcatcaggcc gtataagtta tctcctcgat
     1141 gtacatggac catccatcac tgtggacaca gcctgcagtg gaggagcagt gtgcatagat
     1201 caagccattg attatctcca gtcctcaagt gctgctgata ccgctattat ttgtgctagt
     1261 aatacccatt gctggccggg gtcatttatg tttctcagcg ctcaaggtat ggtttcatct
     1321 gggggtagat gtgctacatt cacgactgat gcagacggat atgttccatc cgaaggagct
     1381 gtagctttca tacttaagac aagagaggct gctatgagag ataaagatac catattggca
     1441 acaataaagg ctacgcaaat ttcccacaac ggacgatccc aaggactcgt ggctcctaac
     1501 gttaatagtc aagccgatct gcatcgtagc ctccttcaga aagccgggct ttcccctgcc
     1561 gatatacatt tcatagaagc acacggcact gggacaagtc tcggagactt gtctgaaatc
     1621 caagccatta acgacgctta tacctctagt caacccagaa cagccggacc attgattgtg
     1681 tccgcctcca aaactgtgat cgggcacact gaacctgctg gaccattggt cggtatgctc
     1741 tctgtactca attcctttaa ggaaggggct gtcccggggc ttgcccacct taccgccgac
     1801 aacttgaatc cggctttgga ttgctctagt gtccctttgt tgattccgta tcaaccggta
     1861 catttggctg cacccaagcc acatagggcc gctgttatga gttatgggtt ctccggtaca
     1921 ctgggaggga tcgtgttgga agctccggat gaagagcgtc ttgaagaaga acccccaaac
     1981 gacaagccaa tgttgtttgt agttagtgct aagacccata ccgccctgat cgagtatttg
     2041 ggtagatatt tggagttttt gcttcaagcc aacccgcaag atttctgcga catatgttac
     2101 acatcatgcg tcggccgtga acattatagg taccgtttcg cttgcgtcgc caacgacatg
     2161 gaggacctga tcggccaact tcaaaaaagg ctggggtcca aggtaccgcc aaaaccgagc
     2221 tataagcgag gcgcacttgc ttttgcattc tccggtcaag gtactcagtt tagaggtatg
     2281 gcaactgaac tggcaaaagc ctactcaggt tttaggaaga tagtcagtga cttggctaag
     2341 agggcctccg aactctccgg ccatgctatt gataggttct tgttggctta tgatattggc
     2401 gctgaaaatg tggcaccaga ctcagaagca gaccaaatat gcatattcgt gtaccaatgc
     2461 agcgtgctca gatggcttca gaccatgggc attcgtccta gtgctgtaat aggtcactca
     2521 ctgggagaaa tctctgcttc agttgcagca ggggcactca gtctcgacag cgccctggat
     2581 ctcgtgataa gcagggctag attgttgaga tcttctacta atgcccccgc tggtatggct
     2641 gccatgtctg ctagccagga tgaggtcgtg gaactcatcg gaaaattgga ccttgataaa
     2701 gctaactcct tgtcagtctc agtcatcaat ggcccccaga acaccgttgt gtccggtagc
     2761 tcagcagcca ttgaatcaat agtagcattg gccaagggta ggaaaattaa agcaagcgct
     2821 cttaatatta atcaggcatt tcactcccca tatgttgatt ctgcagtacc gggcttgcga
     2881 gcctggtcag aaaagcatat ctcatccgca aggccgctcc agataccgtt gtattcaacc
     2941 ttgcttggtg ctcaagtcag cgaagggcaa atgcttaatc cggatcactg ggtagatcat
     3001 gcccgtaagc ctgttcagtt tgctcaagcc gcaacaatca tgaaagagtc ctttactggg
     3061 gtgatcatcg acatcggtcc ccaggtggtt gcctggtcac tgctcttgag caacggtttg
     3121 acatccgtca ctgcactcgc cgctaagcgt gggcgaagcc aacaggtcgc tttcctcagc
     3181 gccttggccg atctgtatca agactatggt gtagtacctg actttgtcgg gttgtacgcc
     3241 cagcaggagg acgctagcag gcttaaaaag acagatattt tgacctaccc gtttcaaagg
     3301 gtcagacgat atccgtcttt cattccgtca cgtcgtgcac cgacacatgc ccacgttcag
     3361 gacgaggaga ctctgtcatc cgggagcagt acgcccacat tggaaaatac cgaccttgat
     3421 tctggtaagg agagtctcat gggaccgaca agagggttgt tgcgtgtcga cgacttgagg
     3481 gactccatcg ttagttctgt gaaggacgta ctcgaactca aatccaacga ggacctcgat
     3541 ttgtcagaat cattgaacgc tcttgggatg gactccatca tgttcgcaca gttgagaaag
     3601 aggataggcg aagggcttgg attgagtgtt cctatggttt ttctgtcaga cgctttctcc
     3661 ataggtgaaa tggttagcaa cctggtggaa caggctgagg cttccgagga caactagctt
     3721 tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata
     3781 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     3841 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3901 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
     3961 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     4021 ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     4081 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     4141 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     4201 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     4261 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg
     4321 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     4381 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     4441 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     4501 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     4561 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     4621 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     4681 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     4741 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     4801 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     4861 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttgga
//
