LOCUS       sequence-335233-        6243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 1 (position 1) vector encoding Kanamycin 
            resistance cassette, for expression in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS
  TITLE     Plants with genetically encoded autoluminescence.
  JOURNAL   Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9.
  PUBMED    32341562
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9."
COMMENT     Sequence Label: pAB120
FEATURES             Location/Qualifiers
     source          1..6243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(131..150)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      944..1532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1433..1452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1868..3016
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    3172..3196
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(3240..3947)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(3974..4768)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4085..4104)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4695..4714
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    4770..4824
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(4855..5034)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5217..5241
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      5333..5964
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6051..6155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6156..6224,6225..6243,1..773)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
       61 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      121 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
      181 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
      241 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
      301 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
      361 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
      421 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
      481 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
      541 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
      601 ctgataaatc tggagccggt gagcgtggtt ctcgcggtat cattgcagca ctggggccag
      661 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
      721 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
      781 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
      841 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
      901 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
      961 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1021 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1081 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1141 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1201 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1261 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1321 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1381 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1441 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1501 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1561 tcctggcaga tcctagatgt ggcgcaacga tgccggcgac aagcaggagc gcaccgactt
     1621 cttccgcatc aagtgttttg gctctcaggc cgaggcccac ggcaagtatt tgggcaaggg
     1681 gtcgctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg acggccagac
     1741 ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca aggcaccagg
     1801 cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg
     1861 agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg
     1921 gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga
     1981 aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg agcgcgacag
     2041 cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg
     2101 tcttgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat
     2161 gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaggccaa
     2221 gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt
     2281 cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc
     2341 cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt
     2401 ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga
     2461 cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat
     2521 caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta
     2581 cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga
     2641 ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg
     2701 caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg
     2761 cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg
     2821 gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg
     2881 cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc
     2941 ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt
     3001 gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag cagccagcgc
     3061 ctgatctggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt
     3121 tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg
     3181 ccaatatatc ctgtcaaaca ctgatagttt aaaccacttc gtgcagaaga caattgcagc
     3241 ggctgagcct cgacatgttg tcgcaaaatt cgccctggac ccgcccaacg atttgtcgtc
     3301 actgtcaagg tttgacctgc acttcatttg gggcccacat acaccaaaaa aatgctgcat
     3361 aattctcggg gcagcaagtc ggttacccgg ccgccgtgct ggaccgggtt gaatggtgcc
     3421 cgtaactttc ggtagagcgg acggccaata ctcaacttca aggaatctca cccatgcgcg
     3481 ccggcgggga accggagttc ccttcagtga gcgttattag ttcgccgctc ggtgtgtcgt
     3541 agatactagc ccctggggca cttttgaaat ttgaataaga tttatgtaat cagtctttta
     3601 ggtttgaccg gttctgccgc tttttttaaa attggatttg taataataaa acgcaattgt
     3661 ttgttattgt ggcgctctat catagatgtc gctataaacc tattcagcac aatatattgt
     3721 tttcatttta atattgtaca tataagtagt agggtacaat cagtaaattg aacggagaat
     3781 attattcata aaaatacgat agtaacgggt gatatattca ttagaatgaa ccgaaaccgg
     3841 cggtaaggat ctgagctaca catgctcagg ttttttacaa cgtgcacaac agaattgaaa
     3901 gcaaatatca tgcgatcata ggcgtctcgc atatctcatt aaagcaggac tctagcgaac
     3961 cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
     4021 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
     4081 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
     4141 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
     4201 cgccatgagt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca
     4261 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
     4321 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
     4381 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
     4441 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
     4501 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
     4561 gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct
     4621 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
     4681 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
     4741 ctgcgtgcaa tccatcttgt tcaaccatgg taattgtaaa tgtaattgta atgttgtttg
     4801 ttgtttgttg ttgttggtaa ttgttgtaaa aataggagag tgaatatgag actctaattg
     4861 gataccgagg ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa tatttgctag
     4921 ctgatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta
     4981 gctcattaaa ctccagaaac ccgcggctca gtggctcctt caacgttgcg gttctgtcag
     5041 ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta
     5101 attctccgct ccggatccga attcggcatt gtcttcacag agtggggccc actgcatcca
     5161 ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     5221 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     5281 aaatcaccac tcgatacagg cagcccatca gtcagatcag gatctccttt gcgacgctca
     5341 ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag
     5401 aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg
     5461 aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac
     5521 ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc
     5581 cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga
     5641 caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat
     5701 gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat
     5761 tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt
     5821 ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg
     5881 tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc
     5941 cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc
     6001 tcggccgcga acggcctcac cccaaaaatg gcagcgctgg ccaattcgtg cgcggaaccc
     6061 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataacctt
     6121 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6181 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6241 tga
//
