LOCUS       sequence-33527-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  TITLE     Gilmore Lab Plasmids
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-FLAG-Nv-IkB
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             90..113
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgcgactctc tggctactga gacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccatgg actacaagga cgacgatgac aagttaacac
      121 gtgctgcaga tgcgaattct tacaagatgg cttctggaga gcaccgaaat ttagttcgca
      181 gaggtgcacc catgattcct gaacacagag cggctttgct cgaaaaatgc cacaaatctg
      241 aacctccaca agggaaagat ttttacgatt caggctttgg gggtagttta gaagaagagg
      301 cagatatttt tgaacgtgaa caagataagc ttgtggaagc aacgagggaa ataacgcttg
      361 atgaatcacc gatatatgga ctcgacaaga aagaaactga acaacctcag tgtacgcctc
      421 cgcctcaaat ggaaagtgat cctatttcag accccgaaat ttcgcagagt cagtcgattt
      481 caccccataa ttgtgtgcta agctacgcag ctggtaatga tgtgcgatgc ttgcttgctc
      541 ctatgagggg cttgttgttc gctcaagacg aagatggcga tactgccctt catctggcca
      601 taatccacac taacgtccaa gcggtggaaa acattgtcgc agctgcacca tctacgaaag
      661 ctcttgatat tttcaactac ttgagacaaa ccccactaca ccttgcaacc atcaccaaac
      721 agtcaaatat tgttcgtggg ctgattgcat ctggtgcaag tgtagacctg gtggatagaa
      781 atggaaaaac cgccctccat ctcgcatgcg aaagaggtga catagacagt gtgcgcgaga
      841 ttataaggcc attatcagat aaggcataca
//
