LOCUS       sequence-335271-        2605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Alpha1 4xLexAOp for assembly of larger LexAOp response elements.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: p4xLexAOp-A1
FEATURES             Location/Qualifiers
     source          1..2605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(406..994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(486..505)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1109..1211
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1212..2027
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1212..2027
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1285..1304)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2141..2596
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2228..2247)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2438..2459
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgtctcagga gagcttgggc tgcaggtcga ggctaaaaaa ctaatcgcat tatcatcccc
       61 tcgacgtact gtacatataa ccactggttt tatatacagc agtactgtac atataaccac
      121 tggttttata tacagcagtc gacgtactgt acatataacc actggtttta tatacagcag
      181 tactgtacat ataaccactg gttttatata cagcagtcga ggtaagatta gatatggata
      241 tgtatatgga tatgtatatg gtggtaatgc catgtaatat gccgctgtca tgagacgaat
      301 tctgacacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      361 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      421 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      481 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      541 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      601 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      661 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      721 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      781 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      841 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      901 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      961 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1021 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1081 aaggatcttc acctagatcc ttttgtgatg atcggcacgt aagaggttcc aactttcacc
     1141 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
     1201 aggaagctaa aatgagccat attcaacggg aaacgtcttg ctcaaggccg cgattaaatt
     1261 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     1321 gtgcgacaat ctaccgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     1381 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     1441 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     1501 tcaccactgc gatcccaggg aaaacagcat tccaggtatt agaagaatat cctgattcag
     1561 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     1621 gtaattgtcc ttttaacagc gatcgcgtat ttcgactcgc tcaggcgcaa tcacgaatga
     1681 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     1741 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     1801 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     1861 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     1921 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     1981 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctgattt tttttaaggc
     2041 agttattggt gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg
     2101 aatggcagaa attcgatgat aagctgtcaa acatgatgtt acgcgccctg tagcggcgca
     2161 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2221 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2281 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2341 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc cagcgccctg atagacggtt
     2401 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2461 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2521 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     2581 ttaacgctta caattttaac gaatt
//
