LOCUS       sequence-335272-        6875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 1 (position 3) vector encoding Neonothopanus 
            nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S 
            promoter, for expression in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS
  TITLE     Plants with genetically encoded autoluminescence.
  JOURNAL   Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9.
  PUBMED    32341562
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9."
COMMENT     Sequence Label: pX029
FEATURES             Location/Qualifiers
     source          1..6875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        111..456
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     399..419
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      1822..2529
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    2578..2602
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(2758..3906)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(4242..4830)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4322..4341)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5001..5792,5793..5861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5624..5643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5862..5966)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6053..6684
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    6776..6800
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caaactagaa ttcgagctcg gaggtcaaca tggtggagca cgacactctg
       61 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
      121 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
      181 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
      241 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
      301 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
      361 gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
      421 tctatataag gaagttcatt tcatttggag aggacacgct cgagtataag agctcatttt
      481 tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc
      541 gatacaatgg cctcttttga aaattcactc tctgtgttga tcgtgggagc cggtttgggt
      601 ggtctagcag ctgctatcgc tctccgacga cagggccacg tggttaagat ctacgactca
      661 tctagcttta aggccgagct cggcgcaggc ttggcagttc caccgaatac actgcgtagt
      721 ctacagcaat tgggttgtaa cactgaaaac cttaacggcg ttgataatct ctgttttact
      781 gccatgggat atgatgggtc agtgggcatg atgaacaata tgactgatta ccgtgaagca
      841 tatggcacta gttggatcat ggttcatcgt gtcgatttgc ataacgagtt gatgagagtt
      901 gctttggacc cagggggatt gggccctccc gcaacgcttc acctgaatca tcgagtcacg
      961 ttttgtgatg tagatgcttg tacggtgaca tttaccaacg gtacaactca aagtgcagat
     1021 cttatcgttg gggctgacgg gatacgttca actatccgta gatttgtctt ggaggaggac
     1081 gtgaccgttc ccgcaagcgg aattgtgggg tttcgatggt tggtacaggc tgatgccctc
     1141 gatccatatc ctgaactcga ttggatagtc aaaaagccac cgctcggagc taggttgatt
     1201 tctacgcccc agaatccaca atctggagtg ggactggcag atcgtagaac tattattata
     1261 tacgcttgtc gtggggggac aatggtaaat gtgctggccg tacacgatga cgagcgagat
     1321 cagaatactg cagattggag cgttccagca tcaaaggatg acttgtttcg agtattccat
     1381 gactaccatc cgagattcag acggctcttg gaattggctc aagatataaa tctctggcag
     1441 atgagggttg tgccggtgct caagaaatgg gtgaacaaac gagtgtgcct gcttggagat
     1501 gccgctcatg cctctcttcc gactctcggc cagggtttcg ggatgggatt ggaggacgcc
     1561 gtagcccttg gtactctctt gccaaaaggt acgactgcta gtcaaataga gacaagactg
     1621 gctgtctacg aacagcttag aaaggaccga gcagagtttg tagccgctga aagttatgaa
     1681 gagcagtatg tccccgagat gaggggcttg tacttgcgta gcaaggaact tagagataga
     1741 gtcatggggt atgacataaa agtggagtct gagaaggtac tggaaacctt gcttaggagc
     1801 tctaactcag cttagcttgt cctgctttaa tgagatatgc gagaagccta tgatcgcatg
     1861 atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat
     1921 ccttaccgcc ggtttcggtt cattctaatg aatatatcac ccgttactat cgtattttta
     1981 tgaataatat tctccgttca atttactgat tgtaccctac tacttatatg tacaatatta
     2041 aaatgaaaac aatatattgt gctgaatagg tttatagcga catctatgat agagcgccac
     2101 aataacaaac aattgcgttt tattattaca aatccaattt taaaaaaagc ggcagaaccg
     2161 gtcaaaccta aaagactgat tacataaatc ttattcaaat ttcaaaagtg ccccaggggc
     2221 tagtatctac gacacaccga gcggcgaact aataacgctc actgaaggga actccggttc
     2281 cccgccggcg cgcatgggtg agattccttg aagttgagta ttggccgtcc gctctaccga
     2341 aagttacggg caccattcaa cccggtccag cacggcggcc gggtaaccga cttgctgccc
     2401 cgagaattat gcagcatttt tttggtgtat gtgggcccca aatgaagtgc aggtcaaacc
     2461 ttgacagtga cgacaaatcg ttgggcgggt ccagggcgaa ttttgcgaca acatgtcgag
     2521 gctcagcagg accgctttac ttgtcttctg cacgaagtgg tttaaactat cagtgtttga
     2581 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
     2641 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     2701 ttccccagat caggcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta
     2761 gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg
     2821 caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga
     2881 tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa
     2941 atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt
     3001 cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg
     3061 ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag
     3121 gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc
     3181 ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta
     3241 gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg
     3301 ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc
     3361 cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg
     3421 cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca
     3481 cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag
     3541 caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg
     3601 cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc
     3661 ctcctgttca agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg
     3721 agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac
     3781 ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc
     3841 ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg
     3901 attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc
     3961 ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc
     4021 ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac
     4081 gaataccagc gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca
     4141 cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta
     4201 ggatctgcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4261 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4321 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4381 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4441 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4501 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4561 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4621 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4681 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4741 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4801 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4861 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4921 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4981 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5041 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5101 gagggcttac catctggccc cagtgctgca atgataccgc gagaaccacg ctcaccggct
     5161 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5221 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5281 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5341 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5401 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5461 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5521 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5581 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5641 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5701 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5761 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5821 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5881 tattgaagca tttatcaagg ttattgtctc atgagcggat acatatttga atgtatttag
     5941 aaaaataaac aaataggggt tccgcgcacg aattggccag cgctgccatt tttggggtga
     6001 ggccgttcgc ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc
     6061 gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg
     6121 cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt
     6181 tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac
     6241 agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc
     6301 aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca
     6361 cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg
     6421 ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct
     6481 gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca
     6541 gtgagggcca agttttccgc gaggtatcca caacgccggc ggccgcggtg tctcgcacac
     6601 ggcttcgacg gcgtttctgg cgcgtttgca gggccataga cggccgccag cccagcggcg
     6661 agggcaacca gcccggtgag cgtcgcaaag gagatcctga tctgactgat gggctgcctg
     6721 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
     6781 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
     6841 ttttaatgta ctggggtgga tgcagtgggc cccac
//
