LOCUS       sequence-335274-        6459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 1 (position 4) vector encoding Neonothopanus 
            nambi luciferase nnLuz with C-terminal His-tag under control of 0.4 
            kb 35S promoter, for expression in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS
  TITLE     Plants with genetically encoded autoluminescence.
  JOURNAL   Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9.
  PUBMED    32341562
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9."
COMMENT     Sequence Label: pX026
FEATURES             Location/Qualifiers
     source          1..6459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(180..971,972..1040))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     803..822
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1041..1145)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1232..1863
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    1955..1979
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        2165..2510
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2453..2473
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             complement(3071..3082)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3408..3425
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3460..4167
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    4216..4240
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(4396..5544)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(join(5880..6459,1..9))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5960..5979)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
       61 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      121 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      181 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      241 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
      301 agtgctgcaa tgataccgcg agaaccacgc tcaccggctc cagatttatc agcaataaac
      361 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
      421 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
      481 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
      541 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
      601 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
      661 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
      721 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
      781 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
      841 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
      901 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
      961 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1021 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcaaggt
     1081 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1141 ccgcgcacga attggccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc
     1201 gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg
     1261 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
     1321 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
     1381 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
     1441 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
     1501 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
     1561 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
     1621 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
     1681 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
     1741 aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc
     1801 gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc
     1861 gtcgcaaagg agatcctgat ctgactgatg ggctgcctgt atcgagtggt gattttgtgc
     1921 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
     1981 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tggggtggat
     2041 gcagtgggcc ccactctgtg aagacaatta cgaattccca tggggaggtc aacatggtgg
     2101 agcacgacac tctggtctac tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg
     2161 ctattgagac ttttcaacaa aggataattt cgggaaacct cctcggattc cattgcccag
     2221 ctatctgtca cttcatcgaa aggacagtag aaaaggaagg tggctcctac aaatgccatc
     2281 attgcgataa aggaaaggct atcattcaag atctctctgc cgacagtggt cccaaagatg
     2341 gacccccacc cacgaggagc atcgtggaaa aagaagaggt tccaaccacg tctacaaagc
     2401 aagtggattg atgtgacatc tccactgacg taagggatga cgcacaatcc cactatcctt
     2461 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgctcgagta
     2521 taagagctca tttttacaac aattaccaac aacaacaaac aacaaacaac attacaatta
     2581 catttacaat tatcgataca atgaggataa atatctcttt gtctagtctc tttgagagac
     2641 tgagcaaatt gtcatcccga tccatcgcaa ttacctgcgg cgtagtcttg gcaagtgcaa
     2701 tagcattccc aattatccga agagactatc aaacgttcct tgaagtcggt ccgagctatg
     2761 ccccacagaa cttccgaggc tatatcatcg tttgtgtctt gtcacttttt aggcaagagc
     2821 agaaagggtt ggcaatctat gacaggcttc cagaaaagag gcgttggctt gccgacttgc
     2881 cgtttcgtga agggacgagg ccatccataa cctcccacat tatacagcgt cagcgtactc
     2941 agctggtaga tcaagagttt gcaaccagag aacttatcga taaggtgatc ccacgtgtgc
     3001 aagcaagaca cacagacaaa acttttctca gtacgtcaaa atttgagttt catgcaaagg
     3061 ccatcttcct tctcccctct atccctatta atgatcctct taacattccc tcacacgata
     3121 cggtaagaag aaccaagcgt gagatcgctc acatgcatga ttaccatgat tgcactctgc
     3181 acttggctct tgctgctcag gatggtaagg aagttttgaa gaaggggtgg ggccagcgtc
     3241 acccactggc cggaccagga gtcccaggcc ctcctactga gtggaccttc ctttacgcac
     3301 caaggaacga agaggaggcc agagttgtcg agatgatagt cgaagctagt attggctaca
     3361 tgactaacga tcctgctggt aagattgtcg aaaatgccaa gtcaggtcac catcaccatc
     3421 accattagtg cgctacatca tgcgctacat cagcttgtcc tgctttaatg agatatgcga
     3481 gaagcctatg atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg
     3541 agcatgtgta gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc
     3601 gttactatcg tatttttatg aataatattc tccgttcaat ttactgattg taccctacta
     3661 cttatatgta caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca
     3721 tctatgatag agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta
     3781 aaaaaagcgg cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt
     3841 caaaagtgcc ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac
     3901 tgaagggaac tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt
     3961 ggccgtccgc tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg
     4021 gtaaccgact tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa
     4081 tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt
     4141 ttgcgacaac atgtcgaggc tcagcaggac cgctcagatt gtcttctgca cgaagtggtt
     4201 taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta
     4261 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     4321 tgcatgccaa ccacagggtt ccccagatca ggcgctggct gctgaacccc cagccggaac
     4381 tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc
     4441 caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt
     4501 cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg
     4561 ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta
     4621 cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc
     4681 gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag
     4741 cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg
     4801 taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag
     4861 gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc
     4921 gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt
     4981 gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg
     5041 cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc
     5101 tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg
     5161 ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc
     5221 ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga
     5281 cttcgccaaa cctgccgcct cctgttcaag acgacgcgaa cgctccacgg cggccgatgg
     5341 cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg
     5401 ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct
     5461 tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc
     5521 cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg
     5581 ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac
     5641 cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt
     5701 ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc cgtgggcctc
     5761 ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc
     5821 atcgttgcgc cacatctagg atctgccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5881 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5941 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6001 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6061 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6121 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6181 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6241 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6301 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6361 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6421 ttttttgttt gcaagcagca gattacgcgc agaaaaaaa
//
