LOCUS       sequence-335275-        6527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 1 (position 5) vector encoding Neonothopanus 
            nambi caffeoylpiruvate hydrolase nnCPH under control of 0.4 kb 35S 
            promoter, for expression in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS
  TITLE     Plants with genetically encoded autoluminescence.
  JOURNAL   Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9.
  PUBMED    32341562
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9."
COMMENT     Sequence Label: pX030
FEATURES             Location/Qualifiers
     source          1..6527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        111..456
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     399..419
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      1474..2181
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    2230..2254
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(2410..3558)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(3894..4482)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3974..3993)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4653..5444,5445..5513))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5276..5295
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5514..5618)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5705..6336
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    6428..6452
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caacagagaa ttcgcatgcg gaggtcaaca tggtggagca cgacactctg
       61 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
      121 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
      181 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
      241 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
      301 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
      361 gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
      421 tctatataag gaagttcatt tcatttggag aggacacgct cgagtataag agctcatttt
      481 tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc
      541 gatacaatgg cacctatttc atccacgtgg agcaggttga taaggttcgt ggctgtagaa
      601 acttccctgg ttcatattgg tgagccaatt gatgcaacga tggacgttgg cctcgcccgt
      661 agagaaggta agacaattca agcatacgaa ataatcggaa gcggcagtgc cctcgatctt
      721 tctgcacaag tctctaagaa cgtactcaca gttagggaat tgttgatgcc gctcagtcga
      781 gaagagatta agaccgttcg atgcctcggc ttgaactatc cggtccacgc cacagaagct
      841 aacgtggccg tgccgaagtt tcctaacctg ttctataagc ccgtaacatc attgattggt
      901 ccaggcggtc ttattactat tccctccgtc gttcaacccc ccaaggagca ccagtcagat
      961 tacgaggctg agctggttat agtaataggt aaggcagcaa agaacgtatc agaggatgag
     1021 gctttggatt atgtattggg ctataccgct gcaaacgata tatctttccg taagcatcaa
     1081 ttggctgtca gtcaatggag cttcagcaag ggctttgatg gaactaatcc acttggtccc
     1141 tgcttggttt ccgcctccag tatccctgac ccacaggata ttccaataca gtgcaaattg
     1201 aatggtggag tcgtccaaaa tggcaacacc agagatcaaa tcttcaacgt taagaaaacc
     1261 atatcatttc tttcccaggg gactactttg gagcccggtt ccattatcct taccgggacc
     1321 ccagatgggg tggggttcgt acgaaacccg cccctgtatt tgaaggatgg cgatgaggtt
     1381 atgacttgga tcggtagtgg aatcggaact ctcgcaaata cagttcgaga agaacaaacg
     1441 tgctttgcat caggcggcca cgaatagctt gtcctgcttt aatgagatat gcgagaagcc
     1501 tatgatcgca tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg
     1561 tgtagctcag atccttaccg ccggtttcgg ttcattctaa tgaatatatc acccgttact
     1621 atcgtatttt tatgaataat attctccgtt caatttactg attgtaccct actacttata
     1681 tgtacaatat taaaatgaaa acaatatatt gtgctgaata ggtttatagc gacatctatg
     1741 atagagcgcc acaataacaa acaattgcgt tttattatta caaatccaat tttaaaaaaa
     1801 gcggcagaac cggtcaaacc taaaagactg attacataaa tcttattcaa atttcaaaag
     1861 tgccccaggg gctagtatct acgacacacc gagcggcgaa ctaataacgc tcactgaagg
     1921 gaactccggt tccccgccgg cgcgcatggg tgagattcct tgaagttgag tattggccgt
     1981 ccgctctacc gaaagttacg ggcaccattc aacccggtcc agcacggcgg ccgggtaacc
     2041 gacttgctgc cccgagaatt atgcagcatt tttttggtgt atgtgggccc caaatgaagt
     2101 gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg gtccagggcg aattttgcga
     2161 caacatgtcg aggctcagca ggaccgcttg tgttgtcttc tgcacgaagt ggtttaaact
     2221 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     2281 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     2341 ccaaccacag ggttccccag atcaggcgct ggctgctgaa cccccagccg gaactgaccc
     2401 cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag
     2461 gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg
     2521 ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg
     2581 gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc
     2641 ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag
     2701 gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga
     2761 caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg
     2821 cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg
     2881 gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc
     2941 caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt
     3001 gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat
     3061 tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat
     3121 cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt
     3181 cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc
     3241 ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc
     3301 caaacctgcc gcctcctgtt caagacgacg cgaacgctcc acggcggccg atggcgcggg
     3361 cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac
     3421 catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat
     3481 ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg
     3541 gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat
     3601 gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc
     3661 ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat
     3721 cttgccctgc acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg
     3781 agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt
     3841 gcgccacatc taggatctgc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     3901 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3961 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4021 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4081 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4141 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4201 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4261 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4321 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4381 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4441 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4501 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4561 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4621 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4681 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4741 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaacca
     4801 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     4861 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     4921 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     4981 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5041 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5101 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5161 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5221 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5281 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5341 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5401 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5461 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5521 ctttttcaat attattgaag catttatcaa ggttattgtc tcatgagcgg atacatattt
     5581 gaatgtattt agaaaaataa acaaataggg gttccgcgca cgaattggcc agcgctgcca
     5641 tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg
     5701 cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac
     5761 gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt
     5821 taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt
     5881 ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc
     5941 ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa
     6001 taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat
     6061 caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc
     6121 tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc
     6181 cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg
     6241 tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc
     6301 agcccagcgg cgagggcaac cagcccggtg agcgtcgcaa aggagatcct gatctgactg
     6361 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
     6421 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
     6481 cattgcggac gtttttaatg tactggggtg gatgcagtgg gccccac
//
