LOCUS       sequence-33528-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  TITLE     Gilmore Lab Plasmids
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-FLAG-Nv-IKK
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttagcgagct ctctggctac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac catggactac aaggacgacg atgacaagtt
      121 aacacgtgct gcagatgcga attccatgga gactcgtagc agttcaaact atatctggaa
      181 tctgaaaagt gtccttggcc aaggagctac aggggcagtg tacacaggtc ggcataagaa
      241 aagtggagat gtagttgctg taaagacatc taaccacttg gggatgatga gacctctaga
      301 agtgcggaaa agagagtttg atgttctgag caaacttgac catgagaaca tcgtcaaaat
      361 cttcgcatct gagaccgagt tgcgatcaca aaatgagata attgtgatgg aattatgttc
      421 tggtggaagc ttgtttacta tgctggagca cccaagcaac gcttatggtc tctctgaaca
      481 agattgcatc actgtcattc gggatgttgt cgctggaatg aagcatcttc atgataatgg
      541 cattgtccat agggatctaa aacctgggaa cattatgagg gtgtaccgag atgacggcag
      601 ctgtgtgtac aagttgacag actttggtgc cgcacgagaa ttgatggaat ctgatcagtt
      661 tgtgtctatc tatggaacag aggagtacct gcatcctgac ttgtatgagc gtggcgtgct
      721 gcgtaaagca accggaaaga catttggagc cc
//
