LOCUS       sequence-335288-       19895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level2 vector for autonomous bioluminescence in 
            plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz
            and nnCPH. Does not contain NpgA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19895)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS
  TITLE     Plants with genetically encoded autoluminescence.
  JOURNAL   Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9.
  PUBMED    32341562
REFERENCE   2  (bases 1 to 19895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 
            10.1038/s41587-020-0500-9."
COMMENT     Sequence Label: pX037
FEATURES             Location/Qualifiers
     source          1..19895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     314..333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             749..1897
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    2043..2067
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        2230..2409
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    2440..2494
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2496..3290
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2550..2569)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3160..3179
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      3317..4024
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        4124..4469
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      9663..10370
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        10475..10820
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      12186..12893
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        12998..13343
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(13904..13915)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             14241..14258
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      14286..14993
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        15098..15443
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      16461..17168
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    17242..17263
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(17278..17283,17284..17301,17302..17308)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17316..17332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     17321..17343
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     17340..17356
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17340..17356
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             17352..17675
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    17369..17425
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(17426..17443)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(17426..17442)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17435..17457)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(17651..17670)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    17807..17831
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      17919..18629
                     /label=oriV
                     /note="incP origin of replication"
     promoter        18650..18754
                     /gene="bla"
                     /label=AmpR promoter
     CDS             18755..19549
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      join(19720..19895,1..413)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
       61 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      121 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      181 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      241 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      301 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      361 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      421 gcaacgcggc ctttttacgg ttcctggcag atcctagatg tggcgcaacg atgccggcga
      481 caagcaggag cgcaccgact tcttccgcat caagtgtttt ggctctcagg ccgaggccca
      541 cggcaagtat ttgggcaagg ggtcgctggt attcgtgcag ggcaagattc ggaataccaa
      601 gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta
      661 tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg
      721 agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag
      781 gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac
      841 cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac
      901 ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc
      961 cgccgtggag cgttcgcgtc gtcttgaaca ggaggcggca ggtttggcga agtcgatgac
     1021 catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc
     1081 aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa
     1141 ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc
     1201 aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc
     1261 gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt
     1321 cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg
     1381 cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg
     1441 gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac
     1501 ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg
     1561 cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga
     1621 aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa
     1681 gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc
     1741 gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg
     1801 gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc
     1861 ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc
     1921 tgggggttca gcagcccctg ctcggatctg ttggaccgga cagtagtcat ggttgatggg
     1981 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
     2041 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
     2101 cggacgtttt taatgtactg gggttgaaca ctctgtgccg aattcggatc cggagcggag
     2161 aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt tttacgtttg
     2221 gaactgacag aaccgcaacg ttgaaggagc cactgagccg cgggtttctg gagtttaatg
     2281 agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta aggtcactat
     2341 cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat tcccctcggt
     2401 atccaattag agtctcatat tcactctcct atttttacaa caattaccaa caacaacaaa
     2461 caacaaacaa cattacaatt acatttacaa ttaccatggt tgaacaagat ggattgcacg
     2521 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     2581 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     2641 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     2701 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     2761 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     2821 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     2881 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     2941 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     3001 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgactcatg
     3061 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     3121 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     3181 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     3241 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     3301 ggggttcgct agagtcctgc tttaatgaga tatgcgagac gcctatgatc gcatgatatt
     3361 tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct cagatcctta
     3421 ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat ttttatgaat
     3481 aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa tattaaaatg
     3541 aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc gccacaataa
     3601 caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa
     3661 acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca ggggctagta
     3721 tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc ggttccccgc
     3781 cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt
     3841 acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga
     3901 attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac
     3961 agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca
     4021 gccgctgcaa gaattcaagc ttggaggtca acatggtgga gcacgacact ctggtctact
     4081 ccaaaaatgt caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa
     4141 ggataatttc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa
     4201 ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta
     4261 tcattcaaga tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     4321 tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct
     4381 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     4441 aaggaagttc atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca
     4501 attaccaaca acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa
     4561 tgaactcaag taagaatccg ccctccacat tgttggatgt atttctcgac acggctagaa
     4621 atcttgatac cgctagtagg aatgtcctgg aatgcgggga acatcgttgg tcatatcgtg
     4681 aattggatac ggtaagctct gccctggccc agcaccttcg atataccgtc ggcttgagtc
     4741 ctacagtagc cgtgatcagt gagaaccacc cttacatatt ggccttgatg ttggccgttt
     4801 ggaaattggg gggtaccttc gctccaatcg acgtacatag tcccgccgag ctcgtggctg
     4861 gcatgttgaa tatagttagc cccagctgtc tggttatacc ttctagtgac gttaccaacc
     4921 agacattggc ctgcgatctc aatataccag tagtagcttt ccaccctcat caatcaacga
     4981 taccggaact gaacaaaaag tacttgactg acagtcaaat atcacctgac ttgcctttcc
     5041 ccgaccccaa caggccggct ctttatctgt ttaccagcag cgctacttct cgttctaatc
     5101 tgaaatgcgt ccccttgacg catacattta tccttagaaa ctctctttca aaaagggcct
     5161 ggtgcaaacg tatgaggccc gaaaccgatt ttgatggtat tcgtgtactc ggttgggctc
     5221 cttggtccca cgtgcttgca catatgcagg acattggacc attgacgctt ttgaacgcag
     5281 ggtgctacgt ttttgctacg actccttcaa catatccgac agagctgaag gacgatcgag
     5341 atgtgatctc ctgtgccgcc aacgcagtca tgtataaggg agttaagtca tttgcctgtc
     5401 taccttttgt gttgggcgga cttaaagcac tctgcgaaag cgagccatct gtaaaagctc
     5461 agttgcaagt ggaagagcga gcacaattgc tcaaatccct gcagcatatg aagatcctgg
     5521 agtgcggtgg tgccatgttg gaagtctccg ttgcatcctg ggcaatagag aacagaatcc
     5581 ccataagcat tgggataggc atgactgaaa ctggtggagc cctcttcgct ggaccagttc
     5641 aggcaataca aacaggattt tcatcagagg acaagttcat agaggacgcc acttatcttt
     5701 tggtgaagga tgactatgag tcccacgccg aggaagatat taacgaaggt gaactcgtcg
     5761 tcaagtccag gatgctgcct cgtgggtatc tggggtataa tgatccatca ttcagcgttg
     5821 atgatgcagg ctgggtgacg ttcaaaacgg gagataggta cagtgttaca cccgacggca
     5881 aatttagctg gctggggaga aataccgact tcattcagat gacatctggt gaaaccttgg
     5941 accctcgtcc gatagaaagt ctcctctgtg agtcctcact tatttctagg gcctgcgtaa
     6001 ttggcgataa gtttcttaac gggccagcaa ctgctgtttg tgccataatc gaactggaac
     6061 caaccaccgt tgagaagggt caagctcatt ccagagacat agctaggata ttcgccccca
     6121 tcaacaggga tttgcccccc cctttgcgta tcgcatggtc acatgtcctg gttttgcagc
     6181 ctagtgagaa gatacctatg acaaagaagg ggacgatttt tcgaaaaaag atcgaacaag
     6241 tctttgggtc cgcattgggt ggatcatccg gcgacaatag ccaagctact actgacgcta
     6301 gcgtggtaag acgtgacgaa ctcagtaaca cagtgaagca cattatatcc cgagtgttgg
     6361 gggtgtctga tgacgaactc ttgtggaccc tctctttcgc cgagttgggt atgacttctg
     6421 ctttggccac caggattgca aatgagttga acgaagtcct tgtgggagtg aacttgccta
     6481 taaacgcctg ctatattcat gtggatcttc cgagcctcag taatgcagtc tatgccaaac
     6541 tcgcacactt gaagttgcca gaccgtactc cggaaccacg aaaggcacca gttgaaaatc
     6601 ccggaggtaa agagatcgtc attgtaggcc aggccttccg tttgcccggg agtatcaacg
     6661 acgtcgcctc tcttagggat gcctttttgg ctaggcaggc cagctcaatt attaccgaga
     6721 tacctccgga caggtgggac cacgcatcct tctacccaaa agatattcgt ttcaataagg
     6781 ctgggctggt ggacatcgcc aactacgatc attccttctt tgggctgacc gccacggaag
     6841 cactctatct cagccctacg atgcgactcg ccttggaggt gtcatttgaa gcattggaga
     6901 atgccaacat tccagtttct caactgaagg gctcccagac agcagtgtat gttgcaacaa
     6961 cagacgatgg ttttgaaacg ttgctcaacg ccgaagcagg ttacgatgcc tacaccagat
     7021 tttacgggac tgggagggct gcctctacgg catcaggccg tataagttat ctcctcgatg
     7081 tacatggacc atccatcact gtggacacag cctgcagtgg aggagcagtg tgcatagatc
     7141 aagccattga ttatctccag tcctcaagtg ctgctgatac cgctattatt tgtgctagta
     7201 atacccattg ctggccgggg tcatttatgt ttctcagcgc tcaaggtatg gtttcatctg
     7261 ggggtagatg tgctacattc acgactgatg cagacggata tgttccatcc gaaggagctg
     7321 tagctttcat acttaagaca agagaggctg ctatgagaga taaagatacc atattggcaa
     7381 caataaaggc tacgcaaatt tcccacaacg gacgatccca aggactcgtg gctcctaacg
     7441 ttaatagtca agccgatctg catcgtagcc tccttcagaa agccgggctt tcccctgccg
     7501 atatacattt catagaagca cacggcactg ggacaagtct cggagacttg tctgaaatcc
     7561 aagccattaa cgacgcttat acctctagtc aacccagaac agccggacca ttgattgtgt
     7621 ccgcctccaa aactgtgatc gggcacactg aacctgctgg accattggtc ggtatgctct
     7681 ctgtactcaa ttcctttaag gaaggggctg tcccggggct tgcccacctt accgccgaca
     7741 acttgaatcc ggctttggat tgctctagtg tccctttgtt gattccgtat caaccggtac
     7801 atttggctgc acccaagcca catagggccg ctgttatgag ttatgggttc tccggtacac
     7861 tgggagggat cgtgttggaa gctccggatg aagagcgtct tgaagaagaa cccccaaacg
     7921 acaagccaat gttgtttgta gttagtgcta agacccatac cgccctgatc gagtatttgg
     7981 gtagatattt ggagtttttg cttcaagcca acccgcaaga tttctgcgac atatgttaca
     8041 catcatgcgt cggccgtgaa cattataggt accgtttcgc ttgcgtcgcc aacgacatgg
     8101 aggacctgat cggccaactt caaaaaaggc tggggtccaa ggtaccgcca aaaccgagct
     8161 ataagcgagg cgcacttgct tttgcattct ccggtcaagg tactcagttt agaggtatgg
     8221 caactgaact ggcaaaagcc tactcaggtt ttaggaagat agtcagtgac ttggctaaga
     8281 gggcctccga actctccggc catgctattg ataggttctt gttggcttat gatattggcg
     8341 ctgaaaatgt ggcaccagac tcagaagcag accaaatatg catattcgtg taccaatgca
     8401 gcgtgctcag atggcttcag accatgggca ttcgtcctag tgctgtaata ggtcactcac
     8461 tgggagaaat ctctgcttca gttgcagcag gggcactcag tctcgacagc gccctggatc
     8521 tcgtgataag cagggctaga ttgttgagat cttctactaa tgcccccgct ggtatggctg
     8581 ccatgtctgc tagccaggat gaggtcgtgg aactcatcgg aaaattggac cttgataaag
     8641 ctaactcctt gtcagtctca gtcatcaatg gcccccagaa caccgttgtg tccggtagct
     8701 cagcagccat tgaatcaata gtagcattgg ccaagggtag gaaaattaaa gcaagcgctc
     8761 ttaatattaa tcaggcattt cactccccat atgttgattc tgcagtaccg ggcttgcgag
     8821 cctggtcaga aaagcatatc tcatccgcaa ggccgctcca gataccgttg tattcaacct
     8881 tgcttggtgc tcaagtcagc gaagggcaaa tgcttaatcc ggatcactgg gtagatcatg
     8941 cccgtaagcc tgttcagttt gctcaagccg caacaatcat gaaagagtcc tttactgggg
     9001 tgatcatcga catcggtccc caggtggttg cctggtcact gctcttgagc aacggtttga
     9061 catccgtcac tgcactcgcc gctaagcgtg ggcgaagcca acaggtcgct ttcctcagcg
     9121 ccttggccga tctgtatcaa gactatggtg tagtacctga ctttgtcggg ttgtacgccc
     9181 agcaggagga cgctagcagg cttaaaaaga cagatatttt gacctacccg tttcaaaggg
     9241 tcagacgata tccgtctttc attccgtcac gtcgtgcacc gacacatgcc cacgttcagg
     9301 acgaggagac tctgtcatcc gggagcagta cgcccacatt ggaaaatacc gaccttgatt
     9361 ctggtaagga gagtctcatg ggaccgacaa gagggttgtt gcgtgtcgac gacttgaggg
     9421 actccatcgt tagttctgtg aaggacgtac tcgaactcaa atccaacgag gacctcgatt
     9481 tgtcagaatc attgaacgct cttgggatgg actccatcat gttcgcacag ttgagaaaga
     9541 ggataggcga agggcttgga ttgagtgttc ctatggtttt tctgtcagac gctttctcca
     9601 taggtgaaat ggttagcaac ctggtggaac aggctgaggc ttccgaggac aactagcttg
     9661 tcctgcttta atgagatatg cgagaagcct atgatcgcat gatatttgct ttcaattctg
     9721 ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt
     9781 tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc
     9841 aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa caatatattg
     9901 tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa caattgcgtt
     9961 ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct aaaagactga
    10021 ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta cgacacaccg
    10081 agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc gcgcatgggt
    10141 gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg gcaccattca
    10201 acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta tgcagcattt
    10261 ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg acgacaaatc
    10321 gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag gaccgctact
    10381 agaattcgag ctcggaggtc aacatggtgg agcacgacac tctggtctac tccaaaaatg
    10441 tcaaagatac agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt
    10501 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
    10561 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag
    10621 atctctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
    10681 aagaagaggt tccaaccacg tctacaaagc aagtggattg atgtgacatc tccactgacg
    10741 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
    10801 catttcattt ggagaggaca cgctcgagta taagagctca tttttacaac aattaccaac
    10861 aacaacaaac aacaaacaac attacaatta catttacaat tatcgataca atggcctctt
    10921 ttgaaaattc actctctgtg ttgatcgtgg gagccggttt gggtggtcta gcagctgcta
    10981 tcgctctccg acgacagggc cacgtggtta agatctacga ctcatctagc tttaaggccg
    11041 agctcggcgc aggcttggca gttccaccga atacactgcg tagtctacag caattgggtt
    11101 gtaacactga aaaccttaac ggcgttgata atctctgttt tactgccatg ggatatgatg
    11161 ggtcagtggg catgatgaac aatatgactg attaccgtga agcatatggc actagttgga
    11221 tcatggttca tcgtgtcgat ttgcataacg agttgatgag agttgctttg gacccagggg
    11281 gattgggccc tcccgcaacg cttcacctga atcatcgagt cacgttttgt gatgtagatg
    11341 cttgtacggt gacatttacc aacggtacaa ctcaaagtgc agatcttatc gttggggctg
    11401 acgggatacg ttcaactatc cgtagatttg tcttggagga ggacgtgacc gttcccgcaa
    11461 gcggaattgt ggggtttcga tggttggtac aggctgatgc cctcgatcca tatcctgaac
    11521 tcgattggat agtcaaaaag ccaccgctcg gagctaggtt gatttctacg ccccagaatc
    11581 cacaatctgg agtgggactg gcagatcgta gaactattat tatatacgct tgtcgtgggg
    11641 ggacaatggt aaatgtgctg gccgtacacg atgacgagcg agatcagaat actgcagatt
    11701 ggagcgttcc agcatcaaag gatgacttgt ttcgagtatt ccatgactac catccgagat
    11761 tcagacggct cttggaattg gctcaagata taaatctctg gcagatgagg gttgtgccgg
    11821 tgctcaagaa atgggtgaac aaacgagtgt gcctgcttgg agatgccgct catgcctctc
    11881 ttccgactct cggccagggt ttcgggatgg gattggagga cgccgtagcc cttggtactc
    11941 tcttgccaaa aggtacgact gctagtcaaa tagagacaag actggctgtc tacgaacagc
    12001 ttagaaagga ccgagcagag tttgtagccg ctgaaagtta tgaagagcag tatgtccccg
    12061 agatgagggg cttgtacttg cgtagcaagg aacttagaga tagagtcatg gggtatgaca
    12121 taaaagtgga gtctgagaag gtactggaaa ccttgcttag gagctctaac tcagcttagc
    12181 ttgtcctgct ttaatgagat atgcgagaag cctatgatcg catgatattt gctttcaatt
    12241 ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc
    12301 ggttcattct aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg
    12361 ttcaatttac tgattgtacc ctactactta tatgtacaat attaaaatga aaacaatata
    12421 ttgtgctgaa taggtttata gcgacatcta tgatagagcg ccacaataac aaacaattgc
    12481 gttttattat tacaaatcca attttaaaaa aagcggcaga accggtcaaa cctaaaagac
    12541 tgattacata aatcttattc aaatttcaaa agtgccccag gggctagtat ctacgacaca
    12601 ccgagcggcg aactaataac gctcactgaa gggaactccg gttccccgcc ggcgcgcatg
    12661 ggtgagattc cttgaagttg agtattggcc gtccgctcta ccgaaagtta cgggcaccat
    12721 tcaacccggt ccagcacggc ggccgggtaa ccgacttgct gccccgagaa ttatgcagca
    12781 tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca aaccttgaca gtgacgacaa
    12841 atcgttgggc gggtccaggg cgaattttgc gacaacatgt cgaggctcag caggaccgct
    12901 ttacgaattc ccatggggag gtcaacatgg tggagcacga cactctggtc tactccaaaa
    12961 atgtcaaaga tacagtctca gaagatcaaa gggctattga gacttttcaa caaaggataa
    13021 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
    13081 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
    13141 aagatctctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
    13201 aaaaagaaga ggttccaacc acgtctacaa agcaagtgga ttgatgtgac atctccactg
    13261 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
    13321 gttcatttca tttggagagg acacgctcga gtataagagc tcatttttac aacaattacc
    13381 aacaacaaca aacaacaaac aacattacaa ttacatttac aattatcgat acaatgagga
    13441 taaatatctc tttgtctagt ctctttgaga gactgagcaa attgtcatcc cgatccatcg
    13501 caattacctg cggcgtagtc ttggcaagtg caatagcatt cccaattatc cgaagagact
    13561 atcaaacgtt ccttgaagtc ggtccgagct atgccccaca gaacttccga ggctatatca
    13621 tcgtttgtgt cttgtcactt tttaggcaag agcagaaagg gttggcaatc tatgacaggc
    13681 ttccagaaaa gaggcgttgg cttgccgact tgccgtttcg tgaagggacg aggccatcca
    13741 taacctccca cattatacag cgtcagcgta ctcagctggt agatcaagag tttgcaacca
    13801 gagaacttat cgataaggtg atcccacgtg tgcaagcaag acacacagac aaaacttttc
    13861 tcagtacgtc aaaatttgag tttcatgcaa aggccatctt ccttctcccc tctatcccta
    13921 ttaatgatcc tcttaacatt ccctcacacg atacggtaag aagaaccaag cgtgagatcg
    13981 ctcacatgca tgattaccat gattgcactc tgcacttggc tcttgctgct caggatggta
    14041 aggaagtttt gaagaagggg tggggccagc gtcacccact ggccggacca ggagtcccag
    14101 gccctcctac tgagtggacc ttcctttacg caccaaggaa cgaagaggag gccagagttg
    14161 tcgagatgat agtcgaagct agtattggct acatgactaa cgatcctgct ggtaagattg
    14221 tcgaaaatgc caagtcaggt caccatcacc atcaccatta gtgcgctacg ctacatcagc
    14281 ttgtcctgct ttaatgagat atgcgagaag cctatgatcg catgatattt gctttcaatt
    14341 ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc
    14401 ggttcattct aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg
    14461 ttcaatttac tgattgtacc ctactactta tatgtacaat attaaaatga aaacaatata
    14521 ttgtgctgaa taggtttata gcgacatcta tgatagagcg ccacaataac aaacaattgc
    14581 gttttattat tacaaatcca attttaaaaa aagcggcaga accggtcaaa cctaaaagac
    14641 tgattacata aatcttattc aaatttcaaa agtgccccag gggctagtat ctacgacaca
    14701 ccgagcggcg aactaataac gctcactgaa gggaactccg gttccccgcc ggcgcgcatg
    14761 ggtgagattc cttgaagttg agtattggcc gtccgctcta ccgaaagtta cgggcaccat
    14821 tcaacccggt ccagcacggc ggccgggtaa ccgacttgct gccccgagaa ttatgcagca
    14881 tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca aaccttgaca gtgacgacaa
    14941 atcgttgggc gggtccaggg cgaattttgc gacaacatgt cgaggctcag caggaccgct
    15001 cagagaattc gcatgcggag gtcaacatgg tggagcacga cactctggtc tactccaaaa
    15061 atgtcaaaga tacagtctca gaagatcaaa gggctattga gacttttcaa caaaggataa
    15121 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
    15181 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
    15241 aagatctctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
    15301 aaaaagaaga ggttccaacc acgtctacaa agcaagtgga ttgatgtgac atctccactg
    15361 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
    15421 gttcatttca tttggagagg acacgctcga gtataagagc tcatttttac aacaattacc
    15481 aacaacaaca aacaacaaac aacattacaa ttacatttac aattatcgat acaatggcac
    15541 ctatttcatc cacgtggagc aggttgataa ggttcgtggc tgtagaaact tccctggttc
    15601 atattggtga gccaattgat gcaacgatgg acgttggcct cgcccgtaga gaaggtaaga
    15661 caattcaagc atacgaaata atcggaagcg gcagtgccct cgatctttct gcacaagtct
    15721 ctaagaacgt actcacagtt agggaattgt tgatgccgct cagtcgagaa gagattaaga
    15781 ccgttcgatg cctcggcttg aactatccgg tccacgccac agaagctaac gtggccgtgc
    15841 cgaagtttcc taacctgttc tataagcccg taacatcatt gattggtcca ggcggtctta
    15901 ttactattcc ctccgtcgtt caacccccca aggagcacca gtcagattac gaggctgagc
    15961 tggttatagt aataggtaag gcagcaaaga acgtatcaga ggatgaggct ttggattatg
    16021 tattgggcta taccgctgca aacgatatat ctttccgtaa gcatcaattg gctgtcagtc
    16081 aatggagctt cagcaagggc tttgatggaa ctaatccact tggtccctgc ttggtttccg
    16141 cctccagtat ccctgaccca caggatattc caatacagtg caaattgaat ggtggagtcg
    16201 tccaaaatgg caacaccaga gatcaaatct tcaacgttaa gaaaaccata tcatttcttt
    16261 cccaggggac tactttggag cccggttcca ttatccttac cgggacccca gatggggtgg
    16321 ggttcgtacg aaacccgccc ctgtatttga aggatggcga tgaggttatg acttggatcg
    16381 gtagtggaat cggaactctc gcaaatacag ttcgagaaga acaaacgtgc tttgcatcag
    16441 gcggccacga atagcttgtc ctgctttaat gagatatgcg agaagcctat gatcgcatga
    16501 tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc
    16561 cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat
    16621 gaataatatt ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa
    16681 aatgaaaaca atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca
    16741 ataacaaaca attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg
    16801 tcaaacctaa aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct
    16861 agtatctacg acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc
    16921 ccgccggcgc gcatgggtga gattccttga agttgagtat tggccgtccg ctctaccgaa
    16981 agttacgggc accattcaac ccggtccagc acggcggccg ggtaaccgac ttgctgcccc
    17041 gagaattatg cagcattttt ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct
    17101 tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat tttgcgacaa catgtcgagg
    17161 ctcagcagga ccgcttgtgt gagaccgcag ctggcacgac aggtttcccg actggaaagc
    17221 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
    17281 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
    17341 aggaaacagc tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagagg
    17401 atccccgggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    17461 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    17521 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
    17581 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
    17641 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
    17701 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
    17761 tgacggtctc agggacacga agtgatccgt ttaaactatc agtgtttgac aggatatatt
    17821 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt
    17881 gaaaaggttt atccgttcgt ccatttgtat gtgccagccg cctttgcgac gctcaccggg
    17941 ctggttgccc tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg
    18001 ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct cgcggaaaac
    18061 ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc
    18121 gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc
    18181 tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc
    18241 ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg
    18301 gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca
    18361 tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc
    18421 ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt
    18481 aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct
    18541 cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc
    18601 cgcgaacggc ctcaccccaa aaatggcagc gctggccaat tcccgagtgc gcggaacccc
    18661 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    18721 ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa tatcaccgga
    18781 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
    18841 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
    18901 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
    18961 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
    19021 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
    19081 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
    19141 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
    19201 cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta aagatccgcg
    19261 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
    19321 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
    19381 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgctcag
    19441 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
    19501 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc tgtcagacca
    19561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    19621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    19681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    19741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    19801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    19861 tactgttctt ctagtgtagc cgtagttagg ccacc
//
