LOCUS       sequence-335349-        8453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PhlF-RFP expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Shao B, Rammohan J, Anderson DA, Alperovich N, Ross D, Voigt CA
  TITLE     Single-cell measurement of plasmid copy number and promoter 
            activity.
  JOURNAL   Nat Commun. 2021 Mar 5;12(1):1475. doi: 10.1038/s41467-021-21734-y.
  PUBMED    33674569
REFERENCE   2  (bases 1 to 8453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-5"; volume: "12(1):1475. doi"; pages: " 
            10.1038/s41467-021-21734-y"
COMMENT     Sequence Label: pSB235
FEATURES             Location/Qualifiers
     source          1..8453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    61..79
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    86..104
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             complement(179..889)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     protein_bind    1328..1346
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1353..1371
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             complement(1520..2143)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     2051..2070
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             complement(2162..3244)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYMTVSRVVNQASHVSAKTREKVEAAMVEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIYSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3206..3225
                     /label=LacI-R
                     /note="LacI, reverse primer"
     misc_feature    3392..3413
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      3416..3459
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(3463..3481)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3621..3640)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3632..4447
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3632..4447
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3705..3724)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(5132..6082)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      6130..6352
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     6886..6903
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6927..6984)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    6985..7005
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(7066..7773)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(7344..7363)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7613..7631
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gtttcgacat cactgtctca tcagaacgca aagcacatgc gttgaccgct gtgctcagta
       61 tctctatcac tgatagggat gtcaatctct atcactgata gggaaaaaac tctagtatct
      121 ccggtagacc agaaacaaaa aaacaccccg aagggtgttc aataattggt tattatcatt
      181 tgtacagttc atccatacca tgcgtgatgc ccgctgcggt tacgaactcc agcagaacca
      241 tatgatcgcg tttctcgttc ggatctttag acagaacgct ttgcgtgctc agatagtgat
      301 tgtctggcag cagaacagga ccatcaccga ttggagtgtt ttgctggtag tgatcagcca
      361 gctgcacgct gccatcctcc acgttgtggc gaattttaaa attcgcttta atgccatttt
      421 tttgtttatc ggcggtgatg taaacattgt cgctgatgta attgtattcc agcttatggc
      481 ccaggatatt gccgtcttct ttaaagtcaa tgcctttcag ctcaatgcgg tttaccaggg
      541 tatcgccttc aaatttcact tccgcacgcg ttttgtacgt gccgtcatcc ttaaaggaaa
      601 tcgtgcgttc ctgcacatag ccttccggca tggcggactt gaagaagtca tgctgcttca
      661 tatggtctgg ataacgagca aagcactgaa caccccaagt cagcgtcgtt accagagtcg
      721 gccaaggtac cggcagttta ccagtagtac agatgaactt cagcgtcagt ttaccattag
      781 ttgcgtcacc ttcaccctcg ccacgcacgg aaaacttatg accgttgaca tcaccatcca
      841 gttccaccag aatagggacg acaccagtga acagctcttc gcctttacgg ctaccgctgc
      901 cgctaccacg gcccagcggc acaaggttga cgacaagatc ttcgacctgc gaggtcgcga
      961 cgagggactt ggtcaaatcg tacaacgatt tgcgcgaggc ctcggtgcta ttcgcaacga
     1021 ttgtcacgtc gtgcgaccat acctgagtgt agcgcacttt cggaagctcg ccgcagacgc
     1081 tggtggagca atcaacgacg tccgcctgat ccagttttag gttgacgcga tacgcggtct
     1141 tggctccgtt ttgacggagc gaagccgtga ggcgcagccg acccaccaga ggcccgacct
     1201 tctcttcgaa gatctgacgg tctgcggtgg actggatctc agtcagagtg cgagtagcct
     1261 cgccgaccga aagaacgatg gttttggaca tctagtattt gacctctttc tcttcccgtg
     1321 ctcattatct ctatcactga tagggatgtc aatctctatc actgataggg aaaaaagcca
     1381 acggtggagt actctagtat gctcatgttt gacagcttat catcgatgca taatgtgcct
     1441 gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccctcgag ccgtcaattg
     1501 tctgattcgt taccaattat taggacccac tttcacattt aagttgtttt tctaatccgc
     1561 atatgatcaa ttcaaggccg aataagaagg ctggctctgc accttggtga tcaaataatt
     1621 cgatagcttg tcgtaataat ggcggcatac tatcagtagt aggtgtttcc ctttcttctt
     1681 tagcgacttg atgctcttga tcttccaata cgcaacctaa agtaaaatgc cccacagcgc
     1741 tgagtgcata taatgcattc tctagtgaaa aaccttgttg gcataaaaag gctaattgat
     1801 tttcgagagt ttcatactgt ttttctgtag gccgtgtacc taaatgtact tttgctccat
     1861 cgcgatgact tagtaaagca catctaaaac ttttagcgtt attacgtaaa aaatcttgcc
     1921 agctttcccc ttctaaaggg caaaagtgag tatggtgcct atctaacatc tcaatggcta
     1981 aggcgtcgag caaagcccgc ttatttttta catgccaata caatgtaggc tgctctacac
     2041 ctagcttctg ggcgagttta cgggttgtta aaccttcgat tccgacctca ttaagcagct
     2101 ctaatgcgct gttaatcact ttacttttat ctaatctgga catttttttc ctcctggatt
     2161 atcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2221 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
     2281 agactggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
     2341 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
     2401 aacatgagct atcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca
     2461 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
     2521 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
     2581 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
     2641 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
     2701 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcctcatggg
     2761 agtaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
     2821 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
     2881 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
     2941 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
     3001 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
     3061 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccacca
     3121 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     3181 gggaaacggt catataagag acaccggcat actctgcgac atcgtataac gttactggtt
     3241 tcatattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     3301 ttttgcgcca ttcgatggcg cgccgccatt ggaccaaaac gaaaaaaggc ccccctttcg
     3361 ggaggcctct tttctggaat ttggtaccga gctctagaag cggccgcgaa ttccagaaat
     3421 catccttagc gaaagctaag gatttttttt atctgaaatt ctgcctcgtg atacgcctat
     3481 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc attgtgtctc
     3541 aaaatctctg atgtacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     3601 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg
     3661 ctccaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     3721 cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc
     3781 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     3841 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     3901 tcctgatgat gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt
     3961 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     4021 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     4081 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     4141 taatggctgg cctgttgaac aagtctggaa agaaatgcat aagcttttgc cattctcacc
     4201 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     4261 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     4321 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     4381 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     4441 tttctaaggt aactgtcaga ccaagtttac tcatatatac tttagattga tttttcgttc
     4501 cactgagcgt cagaccccct gtcagaccaa gtttactcat atatacttta gattgattta
     4561 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tcggtacggg
     4621 ttttgctgcc cgcaaacggg ctgttctggt gttgctagtt tgttatcaga atcgcagatc
     4681 cggcttcagc cggtttgccg gctgaaagcg ctatttcttc cagaattgcc atgatttttt
     4741 ccccacggga ggcgtcactg gctcccgtgt tgtcggcagc tttgattcga taagcagcat
     4801 cgcctgtttc aggctgtcta tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc
     4861 aatttcatgt tctagttgct ttgttttact ggtttcacct gttctattag gtgttacatg
     4921 ctgttcatct gttacattgt cgatctgttc atggtgaaca gctttgaatg caccaaaaac
     4981 tcgtaaaagc tctgatgtat ctatcttttt tacaccgttt tcatctgtgc atatggacag
     5041 ttttcccttt gatatgtaac ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc
     5101 ttcactgata gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt
     5161 tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatact
     5221 tactttgcat gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta
     5281 aagcatcgtg tagtgttttt cttagtccgt tatgtaggta ggaatctgat gtaatggttg
     5341 ttggtatttt gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca
     5401 tttgtctatc tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa
     5461 ccaccaattt catattgctg taagtgttta aatctttact tattggtttc aaaacccatt
     5521 ggttaagcct tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat
     5581 caaggctaat ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc
     5641 actcataaat cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt
     5701 ttatgaattt ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta
     5761 atttttcgct tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca
     5821 aaggattcct gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac
     5881 cataagcatt ttccctactg atgttcatca tctgagcgta ttggttataa gtgaacgata
     5941 ccgtccgttc tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata
     6001 aaattagctt ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt
     6061 tgaaaacaac taattcagac atacatctca attggtctag gtgattttaa tcactatacc
     6121 aattgagatg ggctagtcaa tgataattac tagtcatttt cctttgagtt gtgggtatct
     6181 gtaaattctg ctagaccttt gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt
     6241 ccgctagacc tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa
     6301 agaaagaata aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt
     6361 agtcagttcc gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca
     6421 gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata
     6481 ttccttttgt ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc
     6541 gctgcgctca cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg
     6601 attcatgcaa ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt
     6661 tttatggcgg gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc
     6721 tctgattttc cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa
     6781 tgcacccagt aaggcagcgg tatcatcaac aggcttaccc gtcttactgt cattaccgcc
     6841 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6901 gaggaagcct gcataacgcg aagtaatctt ttcggtttta aagaaaaagg gcagggtggt
     6961 gacaccttgc ccttttttgc cggactgcag cggccgctac tagtaccttt ggtcgaaaaa
     7021 aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt ccttattact tgtacagctc
     7081 gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg tactgttcca cgatggtgta
     7141 gtcctcgttg tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg
     7201 cacgggcttc ttggccttgt aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt
     7261 cagcttcagc ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc
     7321 ggaggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg aggggaagtt
     7381 ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc tgcaaggagg agtcctgggt
     7441 cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg
     7501 gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc
     7561 gtacatgaac tgaggggaca ggatgtccca ggcgaagggc agggggccac ccttggtcac
     7621 cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc
     7681 gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga actccttgat
     7741 gatggccatg ttatcctcct cgcccttgct cacgctaccg ctgccgctac cacgctgtgt
     7801 acccggacaa acaccattaa tcagcaggaa ggtaaattct tcaatatcct gttcaacggt
     7861 cagctgttcg gtcagcaggc gataccaaca aaaaccaaaa atcatatcca gcagcagttc
     7921 acgattggta tctttcggca gttcaccatt gctaatggca ttttcaacca gttttttcgg
     7981 catctcacga cgacgttcca taaactgatc tttcagctgg gtcagggttg cagggtccag
     8041 ctgtgcttct gcaataacac aacgaaatgc ttcaccacaa atggtttcac gccaaacttt
     8101 ccacagatta cgcagcagaa aatccagatc ggctttaaag ctacccagat ccggaaattt
     8161 acgcacctgt tcgctttcat tttcatacac ttcggcaatc agtgctgctt tattggtcca
     8221 ccaacgataa atggtcggtt tgcttgcacc ggcacgacgt gcaacgcttt caatgctcag
     8281 accgctataa ccacattctt tcaggatttc aatggtgctg gtcagaattg ctttatgggt
     8341 atgcggacta cgcaggctac caatgctgct acggctcggg gtacgtgcca tctagtattt
     8401 cagcctttca aacatagtcc atagctcctt aaacaaaatt atttgtagag gcg
//
