LOCUS       sequence-335355-        8449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PP7-CFP and PhlF-RFP expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Shao B, Rammohan J, Anderson DA, Alperovich N, Ross D, Voigt CA
  TITLE     Single-cell measurement of plasmid copy number and promoter 
            activity.
  JOURNAL   Nat Commun. 2021 Mar 5;12(1):1475. doi: 10.1038/s41467-021-21734-y.
  PUBMED    33674569
REFERENCE   2  (bases 1 to 8449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-5"; volume: "12(1):1475. doi"; pages: " 
            10.1038/s41467-021-21734-y"
COMMENT     Sequence Label: pSB233
FEATURES             Location/Qualifiers
     source          1..8449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             741..1448
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(883..901)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1151..1170
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1509..1529
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1530..1587
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1611..1628)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      2162..2384
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2432..3382
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             complement(join(3996..4787,4788..4856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4619..4638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4857..4961)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5029..5047
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(5051..5094)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    5097..5118
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             5266..6348
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYMTVSRVVNQASHVSAKTREKVEAAMVEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIYSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5285..5304)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             6367..6990
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(6440..6459)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    7139..7157
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7164..7182
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             7621..8331
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     protein_bind    8406..8424
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8431..8449
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tactgagcac agcggtcaac gcatgtgctt tgcgttctga tgagacagtg atgtcgaaac
       61 cgcctctaca aataattttg tttaaggagc tatggactat gtttgaaagg ctgaaatact
      121 agatggcacg taccccgagc cgtagcagca ttggtagcct gcgtagtccg catacccata
      181 aagcaattct gaccagcacc attgaaatcc tgaaagaatg tggttatagc ggtctgagca
      241 ttgaaagcgt tgcacgtcgt gccggtgcaa gcaaaccgac catttatcgt tggtggacca
      301 ataaagcagc actgattgcc gaagtgtatg aaaatgaaag cgaacaggtg cgtaaatttc
      361 cggatctggg tagctttaaa gccgatctgg attttctgct gcgtaatctg tggaaagttt
      421 ggcgtgaaac catttgtggt gaagcatttc gttgtgttat tgcagaagca cagctggacc
      481 ctgcaaccct gacccagctg aaagatcagt ttatggaacg tcgtcgtgag atgccgaaaa
      541 aactggttga aaatgccatt agcaatggtg aactgccgaa agataccaat cgtgaactgc
      601 tgctggatat gatttttggt ttttgttggt atcgcctgct gaccgaacag ctgaccgttg
      661 aacaggatat tgaagaattt accttcctgc tgattaatgg tgtttgtccg ggtacacagc
      721 gtggtagcgg cagcggtagc gtgagcaagg gcgaggagga taacatggcc atcatcaagg
      781 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
      841 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      901 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
      961 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     1021 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     1081 actcctcctt gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     1141 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     1201 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     1261 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     1321 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     1381 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     1441 acaagtaata aggaaacaca gaaaaaagcc cgcacctgac agtgcgggct ttttttttcg
     1501 accaaaggta ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc
     1561 tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact
     1621 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1681 atgacagtaa gacgggtaag cctgttgatg ataccgctgc cttactgggt gcattagcca
     1741 gtctgaatga cctgtcacgg gataatccga agtggtcaga ctggaaaatc agagggcagg
     1801 aactgctgaa cagcaaaaag tcagatagca ccacatagca gacccgccat aaaacgccct
     1861 gagaagcccg tgacgggctt ttcttgtatt atgggtagtt tccttgcatg aatccataaa
     1921 aggcgcctgt agtgccattt acccccattc actgccagag ccgtgagcgc agcgaactga
     1981 atgtcacgaa aaagacagcg actcaggtgc ctgatggtcg gagacaaaag gaatattcag
     2041 cgatttgccc gagcttgcga gggtgctact taagccttta gggttttaag gtctgttttg
     2101 tagaggagca aacagcgttt gcgacatcct tttgtaatac tgcggaactg actaaagtag
     2161 tgagttatac acagggctgg gatctattct ttttatcttt ttttattctt tctttattct
     2221 ataaattata accacttgaa tataaacaaa aaaaacacac aaaggtctag cggaatttac
     2281 agagggtcta gcagaattta caagttttcc agcaaaggtc tagcagaatt tacagatacc
     2341 cacaactcaa aggaaaagga ctagtaatta tcattgacta gcccatctca attggtatag
     2401 tgattaaaat cacctagacc aattgagatg tatgtctgaa ttagttgttt tcaaagcaaa
     2461 tgaactagcg attagtcgct atgacttaac ggagcatgaa accaagctaa ttttatgctg
     2521 tgtggcacta ctcaacccca cgattgaaaa ccctacaagg aaagaacgga cggtatcgtt
     2581 cacttataac caatacgctc agatgatgaa catcagtagg gaaaatgctt atggtgtatt
     2641 agctaaagca accagagagc tgatgacgag aactgtggaa atcaggaatc ctttggttaa
     2701 aggctttgag attttccagt ggacaaacta tgccaagttc tcaagcgaaa aattagaatt
     2761 agtttttagt gaagagatat tgccttatct tttccagtta aaaaaattca taaaatataa
     2821 tctggaacat gttaagtctt ttgaaaacaa atactctatg aggatttatg agtggttatt
     2881 aaaagaacta acacaaaaga aaactcacaa ggcaaatata gagattagcc ttgatgaatt
     2941 taagttcatg ttaatgcttg aaaataacta ccatgagttt aaaaggctta accaatgggt
     3001 tttgaaacca ataagtaaag atttaaacac ttacagcaat atgaaattgg tggttgataa
     3061 gcgaggccgc ccgactgata cgttgatttt ccaagttgaa ctagatagac aaatggatct
     3121 cgtaaccgaa cttgagaaca accagataaa aatgaatggt gacaaaatac caacaaccat
     3181 tacatcagat tcctacctac ataacggact aagaaaaaca ctacacgatg ctttaactgc
     3241 aaaaattcag ctcaccagtt ttgaggcaaa atttttgagt gacatgcaaa gtaagtatga
     3301 tctcaatggt tcgttctcat ggctcacgca aaaacaacga accacactag agaacatact
     3361 ggctaaatac ggaaggatct gaggttctta tggctcttgt atctatcagt gaagcatcaa
     3421 gactaacaaa caaaagtaga acaactgttc accgttacat atcaaaggga aaactgtcca
     3481 tatgcacaga tgaaaacggt gtaaaaaaga tagatacatc agagctttta cgagtttttg
     3541 gtgcattcaa agctgttcac catgaacaga tcgacaatgt aacagatgaa cagcatgtaa
     3601 cacctaatag aacaggtgaa accagtaaaa caaagcaact agaacatgaa attgaacacc
     3661 tgagacaact tgttacagct caacagtcac acatagacag cctgaaacag gcgatgctgc
     3721 ttatcgaatc aaagctgccg acaacacggg agccagtgac gcctcccgtg gggaaaaaat
     3781 catggcaatt ctggaagaaa tagcgctttc agccggcaaa ccggctgaag ccggatctgc
     3841 gattctgata acaaactagc aacaccagaa cagcccgttt gcgggcagca aaacccgtac
     3901 cgattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagaa
     4141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatataacc cactcgtgca
     4741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4981 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5041 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
     5101 tcgcggccgc ttctagagct cggtaccaaa ttccagaaaa gaggcctccc gaaagggggg
     5161 ccttttttcg ttttggtcca atggcggcgc gccatcgaat ggcgcaaaac ctttcgcggt
     5221 atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatatgaa accagtaacg
     5281 ttatacgatg tcgcagagta tgccggtgtc tcttatatga ccgtttcccg cgtggtgaac
     5341 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggtggagctg
     5401 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc
     5461 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct
     5521 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa
     5581 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac
     5641 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg
     5701 ttatttcttg atgtctctga ccagacaccc atcaacagta ttatttactc ccatgaggac
     5761 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta
     5821 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc
     5881 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt
     5941 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc
     6001 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt
     6061 gcggatatct cggtagtggg atacgacgat accgaagata gctcatgtta tatcccgccg
     6121 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg
     6181 caactctctc agggccaggc ggtgaagggc aatcagctgt tgccagtctc actggtgaaa
     6241 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     6301 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgata atccaggagg
     6361 aaaaaaatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat
     6421 gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag
     6481 cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag
     6541 atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt
     6601 ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa
     6661 gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc
     6721 tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg
     6781 cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg
     6841 gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat
     6901 caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa
     6961 aaacaactta aatgtgaaag tgggtcctaa taattggtaa cgaatcagac aattgacggc
     7021 tcgagggagt agcatagggt ttgcagaatc cctgcttcgt ccatttgaca ggcacattat
     7081 gcatcgatga taagctgtca aacatgagca tactagagta ctccaccgtt ggcttttttc
     7141 cctatcagtg atagagattg acatccctat cagtgataga gataatgagc acgggaagag
     7201 aaagaggtca aatactagat gtccaaaacc atcgttcttt cggtcggcga ggctactcgc
     7261 actctgactg agatccagtc caccgcagac cgtcagatct tcgaagagaa ggtcgggcct
     7321 ctggtgggtc ggctgcgcct cacggcttcg ctccgtcaaa acggagccaa gaccgcgtat
     7381 cgcgtcaacc taaaactgga tcaggcggac gtcgttgatt gctccaccag cgtctgcggc
     7441 gagcttccga aagtgcgcta cactcaggta tggtcgcacg acgtgacaat cgttgcgaat
     7501 agcaccgagg cctcgcgcaa atcgttgtac gatttgacca agtccctcgt cgcgacctcg
     7561 caggtcgaag atcttgtcgt caaccttgtg ccgctgggcc gtggtagcgg cagcggtagc
     7621 cgtaaaggcg aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat
     7681 gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa
     7741 ctgacgctga agttcatctg tactactggt aaactgccgg taccttggcc gactctggta
     7801 acgacgctga cttggggtgt tcagtgcttt gctcgttatc cagaccatat gaagcagcat
     7861 gacttcttca agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag
     7921 gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac
     7981 cgcattgagc tgaaaggcat tgactttaaa gaagacggca atatcctggg ccataagctg
     8041 gaatacaatt acatcagcga caatgtttac atcaccgccg ataaacaaaa aaatggcatt
     8101 aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac
     8161 taccagcaaa acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg
     8221 agcacgcaaa gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg
     8281 gagttcgtaa ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgataataa
     8341 ccaattattg aacacccttc ggggtgtttt tttgtttctg gtctaccgga gatactagag
     8401 ttttttccct atcagtgata gagattgaca tccctatcag tgatagaga
//
