LOCUS       sequence-33536-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Sullivan JC, Wolenski FS, Reitzel AM, French CE, Traylor-Knowles N, 
            Gilmore TD, Finnerty JR
  TITLE     Two alleles of NF-kappaB in the sea anemone Nematostella vectensis 
            are widely dispersed in nature and encode proteins with distinct 
            activities.
  JOURNAL   PLoS One. 2009  . 4(10):e7311.
  PUBMED    19806194
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2009  . 4(10):e7311."
COMMENT     Sequence Label: pcDNA-FLAG-Nv-NF-kB-Cys
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aagcgactct ctggctactg agacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccatg gactacaagg acgacgatga caagttaaca
      121 cgtgctgcag atgcgaattc gacacagtct gaacagcaag tgggtagtgc tctcacagaa
      181 agcatgttaa atgagatcat tcaacctggt tatctaccag atatcagtgc tctgcatgtc
      241 ccattaggca caaatgcaga ggagccttct tacaccgagc cctacctaga aattcttgaa
      301 cagcctaaac cgcgtggttt ccggttccga tacccatgcg aagggccttc ccatggggga
      361 ctccctgggc agttttcgac ttctaaaagc aagtcatacc cgtctgtcca ggtgaacaac
      421 taccaaggac catgtagaat agttgtaacc ttggttacaa aagatgaacc ttatatgcta
      481 catgcacata gtttaactgg gaagaatgct aatgaagaag gagtagtcac cgtgcaagtg
      541 gggccggatc aacacatgac agcgagcttc ccaaacttgg gaatccagca tgtcactaaa
      601 aagaatgttg ttaaagtact aatggataga tttataaagt ggcaaactct gcaaaatgca
      661 actttcgcca aattgtcaga aggtatcaag gatggtgtag acctatcact ctttggtgtc
      721 aatacagcca taaactcaaa caagcttggg tttgacaaaa gtgtggcgct ctctgtcgca
      781 aacgaagaag ttgccaaaag tagagagtac gctgagcaac aggccgctgc catggatcta
      841 agtgctgtga gactctgctt ccaggcatac ctaccagacc aagacggcaa ctttaccaga
      901 cctctgaagc cagtgtactc agatgctgtc ttggactcca aagcaccatc agcatctcaa
      961 t
//
