LOCUS       sequence-335362-       10439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding the CAG promoter based eccDNA biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10439)
  AUTHORS   Moller HD, Lin L, Xiang X, Petersen TS, Huang J, Yang L, Kjeldsen E,
            Jensen UB, Zhang X, Liu X, Xu X, Wang J, Yang H, Church GM, Bolund 
            L, Regenberg B, Luo Y
  TITLE     CRISPR-C: circularization of genes and chromosome by CRISPR in human
            cells.
  JOURNAL   Nucleic Acids Res. 2018 Dec 14;46(22):e131. doi: 10.1093/nar/gky767.
  PUBMED    30551175
REFERENCE   2  (bases 1 to 10439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-12-14"; volume: "46(22):e131. doi"; pages: 
            " 10.1093/nar/gky767"
COMMENT     Sequence Label: pPB-CAG-ECC-Biosensor
FEATURES             Location/Qualifiers
     source          1..10439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          1802..2030
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     enhancer        2310..2595
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     polyA_signal    2672..2896
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2885..2902
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     2915..2931
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2915..2931
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(2956..3669,3670..3672,3673..3675))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2959..3669,3670..3672,3673..3675))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3001..3022)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3350..3369
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3609..3630
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3672..3681
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3746..3755
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3752..4462
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3897..3915)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4165..4184
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4484..4503)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4528..4576
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        4808..5019
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5035..5634
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5035..5054)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5531..5551
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5653..5774
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     5744..5763
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6537..6571
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7062..7078)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7062..7078)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7075..7097)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7086..7102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7110..7116,7117..7134,7135..7140))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7155..7176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7293..7310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7464..8052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7544..7563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8223..9014,9015..9083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8846..8865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9084..9188)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9214..9669)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9351..9372)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9563..9582
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9796..9818
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9810..9827
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9811..9827
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   9957..10019
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc crggsggggs gggggggggg gggggggggg
      541 ggggggsgrg gsggrgrggk gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tttgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaatct agataccctc
     1741 gtaaaggtct agagctagcg aattcggggc acagagcccc agagaccggc accggtccaa
     1801 cctgaaaaaa agtgatttca ggcaggtgct ccaggtaatt aaacattaat accccaccaa
     1861 ccaaccatcc cttaaaccct tacctcttgc tcagctaatt acagcccgga ggagaagggc
     1921 cgtcccgccc gctcacctgt gggagtaacg cggtcagtca gagccggggc gggcggcgcg
     1981 aggcggcggc ggagcggggc acggggcgaa ggcagcgtcg cagcgactcc cgcccgccgc
     2041 gcgcttcgct ttttataggg ccgccgccgc cgccgcctcg ccataaaagg aaactttcgg
     2101 agcgcgccgc tctgattggc tgccgccgcm ccycyccscc ycsccccscc cccccccccc
     2161 cccccccccc ccccccccgg cgcgcgcccc cccccccccc ccgcccccat cgctgcacaa
     2221 aataattaaa aaataaataa atacaaaatt gggggtgggg agggggggga gatggggaga
     2281 gtgaagcaga acgtggggct cacctcgacc atggtaatag cgatgactaa tacgtagatg
     2341 tactgccaag taggaaagtc ccataaggtc atgtactggg cacaatgcca ggcgggccat
     2401 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     2461 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
     2521 gttactattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
     2581 gtaagttatg taacgggtac ctctagagcc atttgtctgc aataaagcaa tagcatcaca
     2641 aatttcacaa ataaagcatt tttttcactg cccatagagc ccaccgcatc cccagcatgc
     2701 ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata
     2761 gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg
     2821 ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg
     2881 caactagaag gcacagtcga ggctgatcgg ccgctctaga actagtggat cccccgggct
     2941 gcatccaacc tcgagttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt
     3001 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga
     3061 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
     3121 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
     3181 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta
     3241 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
     3301 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
     3361 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
     3421 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt
     3481 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
     3541 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg
     3601 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
     3661 gcccttgctc accatggtgg cggtgcccgg gagacgccaa ccgcaagccg ggacgcgttc
     3721 aagcttcgcc tggagacgat ccgccgccac catggtgagc aagggcgagg aggataacat
     3781 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     3841 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     3901 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     3961 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     4021 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     4081 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     4141 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     4201 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     4261 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     4321 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     4381 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     4441 catggacgag ctgtacaagt aagtttaaac gggggaggct aactgaaaca cggaaggaga
     4501 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt
     4561 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     4621 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     4681 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     4741 gcagatctgc gaaatcggat ccgcggccgc actgattcta gctgcagagg ctccggtgcc
     4801 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     4861 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     4921 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     4981 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg atcctcgagc caccatgacc
     5041 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc
     5101 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac
     5161 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5221 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     5281 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     5341 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     5401 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     5461 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     5521 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     5581 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaatctag
     5641 tgtcgacaag ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5701 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5761 atcaatgtat cttatcatgt ctggaattga ctcaaatgat gtcaattagt ctatcagaag
     5821 ctcatctggt ctcccttccg ggggacaaga catccctgtt taatatttaa acagcagtgt
     5881 tcccaaactg ggttcttata tcccttgctc tggtcaacca ggttgcaggg tttcctgtcc
     5941 tcacaggaac gaagtcccta aagaaacagt ggcagccagg tttagccccg gaattgactg
     6001 gattcctttt ttagggccca ttggtatggc tttttccccg tatcccccca ggtgtctgca
     6061 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc
     6121 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg
     6181 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg
     6241 gctttggggg ggggctgtcc ctgatatcta taacaagaaa atatatatat aataagttat
     6301 cacgtaagta gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca
     6361 cgtaaaagat aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga
     6421 cacttaccgc attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta
     6481 aatgcacagc gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg
     6541 cgtcaatttt acgcagacta tctttctagg gttaatctag ctgcatcagg atcatatcgt
     6601 cgggtctttt ttccggctca gtcatcgccc aagctggcgc tatctgggca tcggggagga
     6661 agaagcccgt gccttttccc gcgaggttga agcggcatgg aaagagtttg ccgaggatga
     6721 ctgctgctgc attgacgttg agcgaaaacg cacgtttacc atgatgattc gggaaggtgt
     6781 ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc acctgggata ccagttcgtc
     6841 gcggcttttc cggacacagt tccggatggt cagcccgaag cgcatcagca acccgaacaa
     6901 taccggcgac agccggaact gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct
     6961 gggatattac gtcagcgagg acgggtatcc tggctggatg ccgcagaaat ggacatggat
     7021 accccgtgag ttacccggcg ggcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     7081 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7141 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7201 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7261 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7321 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7381 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7441 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7501 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7561 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7621 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7681 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7741 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7801 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7861 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7921 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7981 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8041 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8101 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8161 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8221 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8281 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8341 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8401 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8461 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8521 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8581 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8641 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8701 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8761 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8821 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8881 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8941 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9001 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9061 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9121 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9181 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     9241 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     9301 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     9361 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     9421 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     9481 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     9541 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     9601 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     9661 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     9721 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     9781 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcctcgt
     9841 tcattcacgt ttttgaaccc gtggaggacg ggcagactcg cggtgcaaat gtgttttaca
     9901 gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa cacgcagcta gattaaccct
     9961 agaaagataa tcatattgtg acgtacgtta aagataatca tgtgtaaaat tgacgcatgt
    10021 gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt aagttttatt
    10081 atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg tttatttatt
    10141 tattaaaaaa aacaaaaact caaaatttct tctataaagt aacaaaactt ttatgaggga
    10201 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca
    10261 gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc
    10321 gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc
    10381 tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaggcc tccacggcc
//
