LOCUS       sequence-335434-       12335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged CAD with mutation of reported S6K, 
            PKA, and Erk1/2 phosphorylation site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A
FEATURES             Location/Qualifiers
     source          1..12335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    227..584
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             643..1059
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     989..1011
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1027..1043
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1069..1443
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1381..1397
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1470..1479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8147..8170
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(8236..8810,8811..8813,8814..8822)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8403..8420)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8630..8649
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8823..9218
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             9389..9858
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(9988..10005)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10159..10747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10239..10258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10911..11702,11703..11771))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11534..11553
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11772..11876)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             join(11906..12335,1..164)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
       61 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
      121 tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg
      181 atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa
      241 cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt
      301 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa
      361 cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga
      421 cctgagtcca aaaatcccga tcgttttgga ctctttggtg caccccccta ataggaggga
      481 tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg
      541 ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt
      601 tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact
      661 cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg
      721 gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac
      781 gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag
      841 gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa
      901 gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct
      961 cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga
     1021 tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc atatgagatc
     1081 ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac aagagttact
     1141 aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca cgaagtctgg
     1201 agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc tcacccttac
     1261 cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga acctcgctgg
     1321 aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga cggcatcgca
     1381 gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc atcctctaga
     1441 ctgccggatc tagctagtta attaaggatc catggcggcc ctagtgttgg aggacgggtc
     1501 ggtcctgcgg ggccagccct ttggggccgc cgtgtcgact gccggggaag tggtgtttca
     1561 aaccggcatg gtcggctacc ccgaggccct cactgatccc tcctacaagg cacagatctt
     1621 agtgctcacc tatcctctga tcggcaacta tggcatcccc ccagatgaaa tggatgagtt
     1681 cggtctctgc aagtggtttg aatcctcggg catccacgta gcagcactgg tagtgggaga
     1741 gtgctgtcct actcccagcc actggagtgc cacccgcacc ctgcatgagt ggctgcagca
     1801 gcatggcatc cctggcttgc aaggagtaga cactcgggag ctgaccaaga agttgcggga
     1861 acaggggtct ctgctgggga agctggtcca gaatggaaca gaaccttcaa gtctgccatt
     1921 cttggacccc aatgcccgcc ccctggtacc agaggtctcc attaagactc cacgggtatt
     1981 caatacaggg ggtgcccctc ggatccttgc tttggactgt ggcctcaagt ataatcagat
     2041 ccgatgcctc tgccagcgtg gggctgaggt cactgtggta ccctgggacc atgcactaga
     2101 cagccaagag tatgagggtc tcttcttaag taatgggcct ggtgaccctg cctcctatcc
     2161 cagtgtcgta tccacactga gccgtgtttt atctgagcct aatccccgac ctgtctttgg
     2221 gatctgcctg ggacaccagc tattggcctt agccattggg gccaagactt acaagatgag
     2281 atatgggaac cgaggccata accagccctg cttgttggtg ggctctgggc gctgctttct
     2341 gacatcccag aaccatgggt ttgctgtgga gacagactca ctgccagcag actgggctcc
     2401 tctcttcacc aacgccaatg atggttccaa tgaaggcatt gtgcacaaca gcttgccttt
     2461 cttcagtgtc cagtttcacc cagagcacca agctggccct tcagatatgg aactgctttt
     2521 cgatatcttt ctggaaactg tgaaagaggc cacagctggg aaccctgggg gccagacagt
     2581 tagagagcgg ctgactgagc gcctctgtcc ccctgggatt cccactcccg gctctggact
     2641 tccaccacca cgaaaggttc tgatcctggg ctcagggggc ctctccattg gccaagctgg
     2701 agaatttgac tactcgggct ctcaggcaat taaggccctg aaggaggaaa acatccagac
     2761 gttgctgatc aaccccaata ttgccacagt gcagacctcc caggggctgg ccgacaaggt
     2821 ctattttctt cccatagcac ctcattatgt aacccaggtg atacgtaatg aacgccccga
     2881 tggtgtgtta ctgacttttg ggggccagac tgctctgaac tgtggtgtgg agctgaccaa
     2941 ggccggggtg ctggctcggt atggggtccg ggtcctgggc acaccagtgg agaccattga
     3001 gctgaccgag gatcgacggg cctttgctgc cagaatggca gagatcggag agcatgtggc
     3061 cccgagcgag gcagcaaatt ctcttgaaca ggcccaggca gccgctgaac ggctggggta
     3121 ccctgtgcta gtgcgtgcag cctttgccct gggtggcctg ggctctggct ttgcctctaa
     3181 cagggaggag ctctctgctc tcgtggcccc agcttttgcc cataccagcc aagtgctagt
     3241 agacaagtct ctgaagggat ggaaggagat tgagtacgag gtggtgagag acgcctatgg
     3301 caactgtgtc acggtgtgta acatggagaa cttggaccca ctgggcatcc acactggtga
     3361 gtccatagtg gtggccccta gccagacact gaatgacagg gagtatcagc tcctgaggca
     3421 gacagctatc aaggtgaccc agcacctggg aattgttggg gagtgcaatg tgcagtatgc
     3481 cttgaaccct gagtctgagc agtattacat cattgaagtg aatgccaggc tctctcgcag
     3541 ctctgccctg gccagtaagg ccacaggtta tccactggct tatgtggcag ccaagctagc
     3601 attgggcatc cctttgcctg agctcaggaa ctctgtgaca gggggtacag cagcctttga
     3661 acccagcgtg gattattgtg tggtgaagat tcctcgatgg gaccttagca agttcctgcg
     3721 agtcagcaca aagattggga gctgcatgaa gagcgttggt gaagtcatgg gcattgggcg
     3781 ttcatttgag gaggccttcc agaaggccct gcgcatggtg gatgagaact gtgtgggctt
     3841 tgatcacaca gtgaaaccag tcagcgatat ggagttggag actccaacag ataagcggat
     3901 ttttgtggtg gcagctgctt tgtgggctgg ttattcagtg gaccgcctgt atgagctcac
     3961 acgcatcgac cgctggttcc tgcaccgaat gaagcgtatc atcgcacatg cccagctgct
     4021 agaacaacac cgtggacagc ctttgccgcc agacctgctg caacaggcca agtgtcttgg
     4081 cttctcagac aaacagattg cccttgcagt tctgagcaca gagctggctg ttcgcaagct
     4141 gcgtcaggaa ctggggatct gtccagcagt gaaacagatt gacacagttg cagctgagtg
     4201 gccagcccag acaaattacc tatacctaac gtattggggc accacccatg acctcacctt
     4261 tcgaacacct catgtcctag tccttggctc tggcgtctac cgtattggct ctagcgttga
     4321 atttgactgg tgtgctgtag gctgcatcca gcagctccga aagatgggat ataagaccat
     4381 catggtgaac tataacccag agacagtcag caccgactat gacatgtgtg atcgactcta
     4441 ctttgatgag atctcttttg aggtggtgat ggacatctat gagctcgaga accctgaagg
     4501 tgtgatccta tccatgggtg gacagctgcc caacaacatg gccatggcgt tgcatcggca
     4561 gcagtgccgg gtgctgggca cctcccctga agccattgac tcggctgaga accgtttcaa
     4621 gttttcccgg ctccttgaca ccattggtat cagccagcct cagtggaggg agctcagtga
     4681 cctcgagtct gctcgccaat tctgccagac cgtggggtac ccctgtgtgg tgcgcccctc
     4741 ctatgtgctg agcggtgctg ctatgaatgt ggcctacacg gatggagacc tggagcgctt
     4801 cctgagcagc gcagcagccg tctccaaaga gcatcccgtg gtcatctcca agttcatcca
     4861 ggaggctaag gagattgacg tggatgccgt ggcctctgat ggtgtggtgg cagccatcgc
     4921 catctctgag catgtggaga atgcaggtgt gcattcaggt gatgcgacgc tggtgacccc
     4981 cccacaagat atcactgcca aaaccctgga gcggatcaaa gccattgtgc atgctgtggg
     5041 ccaggagcta caggtcacag gacccttcaa tctgcagctc attgccaagg atgaccagct
     5101 gaaagttatt gaatgcaacg tacgtgtctc tcgctccttc cccttcgttt ccaagacact
     5161 gggtgtggac ctagtagcct tggccacgcg ggtcatcatg ggggaagaag tggaacctgt
     5221 ggggctaatg actggttctg gagttgtggg agtaaaggtg cctcagttct ccttctcccg
     5281 cttggcgggt gctgacgtgg tgttgggtgt ggaaatgacc agtactgggg aggtggccgg
     5341 ctttggggag agccgctgtg aggcatacct caaggccatg ctaagcactg gctttaagat
     5401 ccccaagaag aatatcctgc tgaccattgg cagctataag aacaaaagcg agctgctccc
     5461 aactgtgcgg ctactggaga gcctgggcta cagcctctat gccagtctcg gcacagctga
     5521 cttctacact gagcatggcg tcaaggtaac agctgtggac tggcactttg aggaggctgt
     5581 ggatggtgag tgcccaccac agcggagcat cctggagcag ctagctgaga aaaactttga
     5641 gctggtgatt aacctgtcaa tgcgtggagc tgggggccgg cgtctcgctt cctttgtcac
     5701 caagggctac cgcacccgac gcttggccgc tgacttctcc gtgcccctaa tcatcgatat
     5761 caagtgcacc aaactctttg tggaggccct aggccagatc gggccagccc ctcctttgaa
     5821 ggtgcatgtt gactgtatga cctcccaaaa gcttgtgcga ctgccgggat tgattgatgt
     5881 ccatgtgcac ctgcgggaac caggtgggac acataaggag gactttgctt caggcacagc
     5941 cgctgccctg gctgggggta tcaccatggt gtgtgccatg cctaataccc ggccccccat
     6001 cattgacgcc cctgctctgg ccctggccca gaagctggca gaggctggcg cccggtgcga
     6061 ctttgcgcta ttccttgggg cctcgtctga aaatgcagga accttgggca ccgtggccgg
     6121 gtctgcagcc gggctgaagc tttacctcaa tgagaccttc tctgagctgc ggctggacag
     6181 cgtggtccag tggatggagc atttcgagac atggccctcc cacctcccca ttgtggctca
     6241 cgcggagcag caaaccgtgg ctgctgtcct catggtggct cagctcactc agcgctcagt
     6301 gcacatatgt cacgtggcac ggaaggagga gatcctgcta attaaagctg caaaggcacg
     6361 gggcttgcca gtgacctgcg aggtggctcc ccaccacctg ttcctaagcc atgatgacct
     6421 ggagcgcctg gggcctggga agggggaggt ccggcctgag cttggctccc gccaggatgt
     6481 ggaagccctg tgggagaaca tggctgtcat cgactgcttt gcctcagacc atgctcccca
     6541 taccttggag gagaagtgtg ggtccaggcc cccacctggg ttcccagggt tagagaccat
     6601 gctgccacta ctcctgacgg ctgtaagcga gggccggctc agcctggacg acctgctgca
     6661 gcgattgcac cacaatcctc ggcgcatctt tcacctgcct ccgcaggagg acacctatgt
     6721 ggaggtggat ctggagcatg agtggacaat tcccagccac atgcccttct ccaaggccca
     6781 ctggacacct tttgaagggc agaaagtgaa gggcaccgtc cgccgtgtgg tcctgcgagg
     6841 ggaggttgcc tatatcgatg ggcaggttct ggtacccccg ggctatggac aggatgtacg
     6901 gaagtggcca cagggggctg ttcctcagct cccaccctca gcccctgcca ctagtgagat
     6961 gaccacgaca cctgaaagac cccgccgtgg catcccaggg cttcctgatg gccgcttcca
     7021 tctgccgccc cgaatccatc gagccgccga cccaggtttg ccagctgagg agccaaagga
     7081 gaagtcctct cggaaggtag ccgagccaga gctgatggga acccctgatg gcacctgcta
     7141 ccctccacca ccagtaccga gacaggcatc tccccagaac ctggggaccc ctggcttgct
     7201 gcacccccag acctcacccc tgctgcactc attagtgggc caacatatcc tgtccgtcca
     7261 gcagttcacc aaggatcaga tgtctcacct gttcaatgtg gcacacacac tgcgtatgat
     7321 ggtgcagaag gagcggagcc tcgacatcct gaaggggaag gtcatggcct ccatgttcta
     7381 tgaagtgagc acacggacca gcagctcctt tgcagcagcc atggcccggc tgggaggtgc
     7441 tgtgctcagc ttctcggaag ccacatcgtc cgtccagaag ggcgaatccc tggctgactc
     7501 cgtgcagacc atgagctgct atgccgacgt cgtcgtgctc cggcaccccc agcctggagc
     7561 agtggagctg gccgccaagc actgccggag gccagtgatc aatgctgggg atggggtcgg
     7621 agagcacccc acccaggccc tgctggacat cttcaccatc cgtgaggagc tgggaactgt
     7681 caatggcatg acgatcacga tggtgggtga cctgaagcac ggacgcacag tacattccct
     7741 ggcctgcctg ctcacccagt atcgtgtcag cctgcgctac gtggcacctc ccagcctgcg
     7801 catgccaccc actgtgcggg ccttcgtggc ctcccgcggc accaagcagg aggaattcga
     7861 gagcattgag gaggcgctgc ctgacactga tgtgctctac atgactcgaa tccagaagga
     7921 acgatttggc tctacccagg agtacgaagc ttgctttggt cagttcatcc tcactcccca
     7981 catcatgacc cgggccaaga agaagatggt ggtgatgcac ccgatgcccc gtgtcaacga
     8041 gataagcgtg gaagtggact cggatccccg cgcagcctac ttccgccagg ctgagaacgg
     8101 catgtacatc cgcatggctc tgttagccac cgtgctgggc cgtttcgact acaaagacga
     8161 tgacgacaag taggaattcc tgcaggcctc gagggccggc gcgccgcggc cgctacgtaa
     8221 attccgcccc ccccccccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     8281 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     8341 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     8401 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     8461 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     8521 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     8581 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     8641 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     8701 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     8761 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     8821 ccatggttcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca
     8881 acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc
     8941 gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg
     9001 tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa
     9061 atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc
     9121 atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc
     9181 gtgaattgct gccctctggt tatgtgtggg agggctaagt cgacgataaa ataaaagatt
     9241 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     9301 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc
     9361 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     9421 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
     9481 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     9541 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     9601 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     9661 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
     9721 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     9781 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     9841 gtcagcgggg gtctttcaca tgcagcatgt atcaaaatta atttggtttt ttttcttaag
     9901 tatttacatt aaatggccat agttgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9961 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    10021 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    10081 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    10141 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    10201 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10261 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10321 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10381 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10441 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10501 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10561 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    10621 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10681 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10741 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10801 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttgcg
    10861 gccggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    10921 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    10981 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    11041 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    11101 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    11161 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    11221 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    11281 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    11341 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    11401 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    11461 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    11521 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    11581 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    11641 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    11701 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    11761 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    11821 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    11881 tttccccgaa aagtgccacc tgcataatga aagaccccac ctgtaggttt ggcaagctag
    11941 cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata gaaaagttca
    12001 gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
    12061 gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca
    12121 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
    12181 tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
    12241 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
    12301 cgcgcgcttc tgctccccga gctcaataaa agagc
//
