LOCUS       sequence-335438-       11372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mEmerald tagged CAPRIN1 under EF-1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11372)
  AUTHORS   Liao YC, Fernandopulle MS, Wang G, Choi H, Hao L, Drerup CM, Patel 
            R, Qamar S, Nixon-Abell J, Shen Y, Meadows W, Vendruscolo M, Knowles
            TPJ, Nelson M, Czekalska MA, Musteikyte G, Gachechiladze MA, 
            Stephens CA, Pasolli HA, Forrest LR, St George-Hyslop P, 
            Lippincott-Schwartz J, Ward ME
  TITLE     RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, 
            Using Annexin A11 as a Molecular Tether.
  JOURNAL   Cell. 2019 Sep 19;179(1):147-164.e20. doi: 
            10.1016/j.cell.2019.08.050.
  PUBMED    31539493
REFERENCE   2  (bases 1 to 11372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08"; journalName: "Cell"; date: "2019-09-19- 
            19"; volume: "179"; issue: "1"; pages: "147-164.e20"
COMMENT     Sequence Label: EF1a-mEmerald-CAPRIN1
FEATURES             Location/Qualifiers
     source          1..11372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             31..75
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    373..490
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        660..871
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          872..1810
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1767..1787
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(1831..1833,1834..1836,1837..2547)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1876..1897)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2137..2156)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2484..2505
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4763..4796)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4852..5440
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4905..4925)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5323..5334)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5443..5459)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5444..5460)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5969..6149
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6211..6799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6291..6310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6970..7761,7762..7830))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7593..7612
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7831..7935)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8010..8029)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8201..8580
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8582..8780
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8735..8755
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8798..8978
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9025..9150
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9647..9880
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
       61 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
      121 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
      181 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
      241 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
      301 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
      361 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      421 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      481 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag taccgggccc
      541 gctctagacg aaagtgtctt accggaacct gaatgcagtc tctcggaggg cgaagaatcc
      601 tgcaggctcg aggcggccgc gtggataacc gtattaccgc catgcatgtg cccgtcagtg
      661 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      721 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      781 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      841 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
      901 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
      961 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     1021 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     1081 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     1141 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     1201 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     1261 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     1321 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     1381 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     1441 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     1501 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     1561 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcgc gcttttggag
     1621 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     1681 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     1741 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     1801 tccatttcag gtgtcgtgag ctagcccacc atggtgagca agggcgagga gctgttcacc
     1861 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1921 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1981 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag
     2041 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2101 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2161 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2221 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag
     2281 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac
     2341 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2401 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa
     2461 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2521 actctcggca tggacgagct gtacaagggt gacccaccgg ttgctactgg aggaggaggt
     2581 tctggaggtg gtggaagtgg tggcggaggt agtatgccat ctgctacctc tcatagtggt
     2641 agtggaagta agtcatcagg accaccacct ccatcaggtt catctggtag tgaagcagct
     2701 gcaggagcag gcgctgcagc tcctgcttct caacacccag caaccggaac tggagctgtc
     2761 cagaccgagg ccatgaagca gattctcggg gtgatcgaca agaaacttcg gaacctggag
     2821 aagaaaaagg gtaagcttga tgattaccag gaacgaatga acaaagggga aaggcttaat
     2881 caagatcagc tggatgccgt ttctaagtac caggaagtca caaataattt ggagtttgca
     2941 aaagaattac agaggagttt catggcacta agtcaagata ttcagaaaac aataaagaag
     3001 acagcacgtc gggagcagct tatgagagaa gaagctgaac agaaacgttt aaaaactgta
     3061 cttgagctac agtatgtttt ggacaaattg ggagatgatg aagtgcggac tgacctgaaa
     3121 caaggtttga atggagtgcc aatattgtcc gaagaggagt tgtcattgtt ggatgaattc
     3181 tataagctag tagaccctga acgggacatg agcttgaggt tgaatgaaca gtatgaacat
     3241 gcctccattc acctgtggga cctgctggaa gggaaggaaa aacctgtatg tggaaccacc
     3301 tataaagttc taaaggaaat tgttgagcgt gtttttcagt caaactactt tgacagcacc
     3361 cacaaccacc agaatgggct gtgtgaggaa gaagaggcag cctcagcacc tgcagttgaa
     3421 gaccaggtac ctgaagctga acctgagcca gcagaagagt acactgagca aagtgaagtt
     3481 gaatcaacag agtatgtaaa tagacagttc atggcagaaa cacagttcac cagtggtgaa
     3541 aaggagcagg tagatgagtg gacagttgaa acggttgagg tggtaaattc actccagcag
     3601 caacctcagg ctgcatcccc ttcagtacca gagccccact ctttgactcc agtggctcag
     3661 gcagatcccc ttgtgagaag acagcgagta caagacctta tggcacaaat gcagggtccc
     3721 tataatttca tacaggattc aatgctggat tttgaaaatc agacacttga tcctgccatt
     3781 gtatctgcac agcctatgaa tccaacacaa aacatggaca tgccccagct ggtttgccct
     3841 ccagttcatt ctgaatctag acttgctcag cctaatcaag ttcctgtaca accagaagcg
     3901 acacaggttc ctttggtatc atccacaagt gaggggtaca cagcatctca acccttgtac
     3961 cagccttctc atgctacaga gcaacgacca cagaaggaac caattgatca gattcaggca
     4021 acaatctctt taaatacaga ccagactaca gcatcatcat cccttcctgc tgcgtctcag
     4081 cctcaagtat ttcaggctgg gacaagcaaa cctttacata gcagtggaat caatgtaaat
     4141 gcagctccat tccaatccat gcaaacggtg ttcaatatga atgccccagt tcctcctgtt
     4201 aatgaaccag aaactttaaa acagcaaaat cagtaccagg ccagttataa ccagagcttt
     4261 tctagtcagc ctcaccaagt agaacaaaca gagcttcagc aagaacagct tcaaacagtg
     4321 gttggcactt accatggttc cccagaccag tcccatcaag tgactggtaa ccaccagcag
     4381 cctcctcagc agaacactgg atttccacgt agcaatcagc cctattacaa tagtcgtggt
     4441 gtgtctcgtg gaggctcccg tggtgctaga ggcttgatga atggataccg gggccctgcc
     4501 aatggattca gaggaggata tgatggttac cgcccttcat tctctaacac tccaaacagt
     4561 ggttatacac agtctcagtt cagtgctccc cgggattact ctggctatca acgggatgga
     4621 tatcagcaga atttcaagcg aggctctggg cagagtggac cacggggagc accacgaggt
     4681 cgtggaggtc caccaagacc taacagaggt atgccacaaa tgaacactca gcaagtgaat
     4741 tgagaattcg tcgagggacc taataacttc gtatagcata cattatacga agttatacat
     4801 gtttaagggt tccggttcca ctaggtacaa ttcgatatca agcttatcga taatcaacct
     4861 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4921 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4981 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5041 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5101 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5161 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5221 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     5281 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5341 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5401 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     5461 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     5521 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     5581 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5641 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     5701 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     5761 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     5821 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     5881 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     5941 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     6001 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6061 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6121 cttttagtca gtgtggaaaa tctctagcag catgtgagca aaaggccagc aaaaggccag
     6181 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6241 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6301 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6361 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6421 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6481 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6541 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6601 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6661 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6721 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6781 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6841 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6901 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6961 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7021 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7081 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7141 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7201 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7261 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7321 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7381 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7441 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7501 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7561 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7621 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7681 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7741 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7801 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7861 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7921 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     7981 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     8041 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     8101 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     8161 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     8221 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     8281 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     8341 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     8401 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     8461 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     8521 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     8581 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     8641 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     8701 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     8761 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
     8821 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8881 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8941 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     9001 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     9061 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     9121 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     9181 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     9241 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     9301 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     9361 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     9421 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9481 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     9541 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     9601 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     9661 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     9721 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     9781 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     9841 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     9901 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     9961 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
    10021 gcttaatact gagagatccc ctcataattt ccccaaaacg taaccatgtg tgaatagatt
    10081 ttgagtaagc agggttgcag ccacgagtga gtcttccctt gttattgtgt agccagaatg
    10141 ccgcaaaact tccatgccta agcgaactgt tgagagtacg tttcgatttc tgactgtgtt
    10201 agcctggaag tgcttgtccc aaccttgttt ctgagcatga acgcccgcaa gccaacatgt
    10261 tagttgaagc atcagggcga ttagcagcat gatatcaaaa cgctctgagc tgctcgttcg
    10321 gctatggcgt aggcctagtc cgtaggcagg acttttcaag tctcggaagg tttcttcaat
    10381 ctgcattcgc ttcgaataga tattaacaag ttgtttgggt gttcgaattt caacaggtaa
    10441 gttagttgct agaatccatg gctcctttgc cgacgctgag tagattttag gtgacgggtg
    10501 gtgacaatga gtccgtgtcg agcgctgatt ttttcggcct ttagagcgag atttatacaa
    10561 tagaatttgg catgagattg gattgctttt agtcagcctc ttatagccta aagtctttga
    10621 gtgactagat gacatatcat gtaagttgct gataggtttc cagttttccg ctcctaggtc
    10681 tgcatattgt acttttcctc ttactcgact taaccagtac caacccagct tctcaacgga
    10741 tttataccat ggcactttaa agccagcatc actgacaatg agcggtgtgg tgttactcgg
    10801 tagaatgctc gcaaggtcgg ctagaaattg gtcatgagct ttctttgaac attgctctga
    10861 aagcgggaac gctttctcat aaagagtaac agaacgaccg tgtagtgcga ctgaagctcg
    10921 caataccata agccgttttt gctcacggat atcagaccag tcaacaagta caatgggcat
    10981 cgtattgccc gaacagataa agctagcatg ccaacggtat acagcgagtc gctctttgtg
    11041 gaggtgacga ttacctaaca atcggtcgat tcgtttgatg ttatgttttg ttctcgcttt
    11101 ggttggcagg ttacggccaa gttcggtaag agtgagagtt ttacagtcaa gtaaggcgtg
    11161 gcaagccaac gttaagctgt tgagtcgttt taagtgtaat tcggggcaga attggtaaag
    11221 agagtcgtgt aaaatatcga gttcgcacat tttgttgtct gattattgat ttttggcgaa
    11281 accatttgat catatgacaa gatgtgtatc taccttaact taatgatttt gataaaaatc
    11341 attaggggat tcatcagagc ttaatacact cc
//
