LOCUS       sequence-335453-        2492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Omega1 4xLexAOp for assembly of larger LexAOp response elements.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: p4xLexAOp-O1
FEATURES             Location/Qualifiers
     source          1..2492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(478..1066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(558..577)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1181..1283
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1284..1943
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1350..1369)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2056..2484
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2143..2162)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2353..2374
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atattaacgc ttacaatttt aacggatccg gtctcaggag gcatgcgtcg actctagacc
       61 cgggcagctg cgctgtcagg agagcttggg ctgcaggtcg aggctaaaaa actaatcgca
      121 ttatcatccc ctcgacgtac tgtacatata accactggtt ttatatacag cagtactgta
      181 catataacca ctggttttat atacagcagt cgacgtactg tacatataac cactggtttt
      241 atatacagca gtactgtaca tataaccact ggttttatat acagcagtcg aggtaagatt
      301 agatatggat atgtatatgg atatgtatat ggtggtaatg ccatgtaata tgccgctgtc
      361 atgagaccgg atcctgacac ggttatccac agaatcaggg gataacgcag gaaagaacat
      421 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      481 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      541 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      601 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      661 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      721 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      781 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      841 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
      901 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
      961 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1021 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1081 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1141 gagattatca aaaaggatct tcacctagat ccttttgtga tgatcggcac gtaagaggtt
     1201 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
     1261 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     1321 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     1381 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     1441 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1501 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1561 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     1621 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1681 atttccctaa agggtttatt gagaatatgt ttttcgtgtc agccaatccc tgggtgagtt
     1741 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1801 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1861 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1921 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
     1981 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg atgataagct
     2041 gtcaaacatg atgttacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2101 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     2161 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     2221 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     2281 gttcacgtag tgggccagcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     2341 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     2401 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     2461 tttaacaaaa atttaacgcg aattttaaca aa
//
