LOCUS       sequence-335472-       10458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mNG^SEC^2xHA vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Duong T, Rasmussen NR, Ballato E, Mote FS, Reiner DJ
  TITLE     The Rheb-TORC1 signaling axis functions as a developmental 
            checkpoint.
  JOURNAL   Development. 2020 Mar 2;147(5). pii: dev.181727. doi: 
            10.1242/dev.181727.
  PUBMED    32041790
REFERENCE   2  (bases 1 to 10458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2020-03-2"; volume: "147(5). pii: dev.181727.
            doi"; pages: " 10.1242/dev.181727"
COMMENT     Sequence Label: pTD31
FEATURES             Location/Qualifiers
     source          1..10458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             661..966
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     regulatory      1344..1353
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1899..1919
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    1937..1970
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             1980..2009
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3826..4149)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6067..6091
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6094..7119
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6094..7119
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7120..7144)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    7497..7530
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             7549..7575
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7576..7602
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(8237..8253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8237..8253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8250..8272)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8261..8277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8285..8291,8292..8309,8310..8315))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8330..8351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8468..8485)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8639..9227)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8719..8738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9398..10189,10190..10258))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10021..10040
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10259..10363)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10431..10449
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcgatg gtctccaagg gagaggagga caacatggcc tccctcccag
     1081 ccacccacga gctccacatc ttcggatcca tcaacggagt cgacttcgac atggtcggac
     1141 aaggaaccgg aaacccaaac gacggatacg aggagctcaa cctcaagtcc accaaggtaa
     1201 gtttaaacat atatatacta actaaccctg attatttaaa ttttcaggga gacctccaat
     1261 tctccccatg gatcctcgtc ccacacatcg gatacggatt ccaccaatac ctcccatacc
     1321 cagacggaat gtccccattc caagccgcca tggtcgacgg atccggatac caagtccacc
     1381 gtaccatgca attcgaggac ggagcctccc tcaccgtcaa ctaccgttac acctacgagg
     1441 gatcccacat caaggtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
     1501 ttcagggaga ggcccaagtc aagggaaccg gattcccagc cgacggacca gtcatgacca
     1561 actccctcac cgccgccgac tggtgccgtt ccaagaagac ctacccaaac gacaaggtaa
     1621 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagacc atcatctcca
     1681 ccttcaagtg gtcctacacc accggaaacg gaaagcgtta ccgttccacc gcccgtacca
     1741 cctacacctt cgccaagcca atggccgcca actacctcaa gaaccaacca atgtacgtct
     1801 tccgtaagac cgagctcaag cactccaaga ccgagctcaa cttcaaggag tggcaaaagg
     1861 ccttcaccga cgtcatggga atggacgagc tctacaagga gaatctgtac tttcaatccg
     1921 gaaaggtaag tttaaaataa cttcgtataa tgtatactat acgaagttat tttcagggag
     1981 agcaaaagct tatctccgag gaggacctct aaggatgatc gacgccaacg tcgttgaatt
     2041 ttcaaatttt aaatactgaa tatttgtttt ttttcctatt atttatttat tctctttgtg
     2101 ttttttttct tgctttctaa aaaattaatt caatccaaat ctaaacattt ttttttctct
     2161 ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca agtttattta
     2221 tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg aaacacacaa
     2281 aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat tcagaaatat
     2341 ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaac tagctgccct
     2401 cgattgtcta tcttccattc tcaccaagtt tcaacaactt tattcagtca ttcttctctg
     2461 gttcatttcc caaccaatct tcccgaataa agcccatctg tcctttcggc cacattcctc
     2521 acctacatcc gtactcctta tctcccgttc ttatccagtc ttttatcgtc tctgcgtctc
     2581 gcacactgtc tcgtcggtaa tgtaaaaaaa aaacaaaaaa aacttgaaaa cttgcggaaa
     2641 gatttcctaa taaaaggaga cgaatccggt aggaaatcga caagtctctg cttctcgaag
     2701 actaggtgag ttacatttct ttggccgaat tattttgaaa tcctaaattt gataatttca
     2761 gagatgtctg taaaacttgc gtgttatgtg acggcttcgg tcactgtcgc cactcttatg
     2821 gtatgcttca tgaccatgtc aaccatctac tcggaggttg atggattcag agagaagctt
     2881 gataccgaga tgaatgtgtt cagagtgagt taaattatat tttgaatttt aaataatttt
     2941 taattttcta gcaatctacc aatggattgt ggaaggacat agttgtcatc ggaagatcta
     3001 gcaagcgtgt ccgttgtcaa tatgaagaga ccaacgctac cccaactcca catgctgatg
     3061 gatccccatc tgctccacca ggtcaaccac cagcagttcc accagtcttc aaccagccaa
     3121 agactccaaa tggagccaat ggaaatggac caacctgcaa ctgcaatgct gataacaagt
     3181 gcccagctgg accatccgga ccaaagggag ttccaggagt tccaggactc gacggagttc
     3241 caggacttga cggtgttcca ggagttggag ctgatgatat cgctccacaa cgcgagtctg
     3301 tcggatgctt cacttgccca caaggaccag ttggaccacc aggagctctt ggaagaccag
     3361 gaccacgtgg acttccagga ccaagaggac aaaatggaaa cccaggaaga gatggacaac
     3421 caggacatcc aggagagcaa ggatcatccg gtcaaatcgg aaagatcgga gagccaggac
     3481 caccaggaga gaagggacgc gacgccgagc atccaatcgg aagaccagga ccaaagggac
     3541 caagaggaga tcaaggacca acaggaccag ctggacagaa cggtcttcac ggaccaccag
     3601 gagagccagg aaccgttgga ccagaaggac catctggaaa gcaaggacgt caaggaccag
     3661 acggaaccca gggagagact ggaccagacg gaagaccagg aaaggatgcc gagtactgcc
     3721 agtgcccaga caagtctcca ccatcagagg ctgtcaacgc caaccgtgga tacagaaata
     3781 tctaaattgt tggtgttttc taataaaata tttgagattc agtgagactt ttttcttggc
     3841 ggcacaataa agtttcctct ttttgttggg tcactcaacg aacacaatac aaatatttga
     3901 gcgctcgcat ttttctcaac ctttcgggtt taaagcactt caaaatggaa ttgaagagag
     3961 attgtgaaag tacatcagac agaatcacat tttgatagca ttcacttcac tcagatgcaa
     4021 acttttcagt gactataaaa agtcataaaa taataacatt aaaaaagcaa ataaattagc
     4081 gagagaattt tttgacaaaa agaaagaaga gtgatagaga agaagggaat gcttgaaagg
     4141 attttgcatt tatcttaatc accgtcttcg agaagacgaa ccatggcgcc agtttcagaa
     4201 tccaagtttc taatatagtt catcacaatg ttgacgttgg tccaacctcc ggcttgcata
     4261 atctctggga tggaaactcc agcgcgagcc atgtcacggg cagctccgac acgggcggaa
     4321 tgtccagacc aagccaagta acgttgtccg gaatcatcct tggctccgta gatgagacgg
     4381 tgggtagctt cgaagattcc tgaaaattta ttttcagctt attcttagct aatatgttgt
     4441 ttaaacttac cttcaagagc tcgagtcgag agttgtgatg ttgcactcgg ggcagcaact
     4501 ccgttcttgc gaacacgaca gaacaggtag ttgtttggat catcggcgac tccggaaacc
     4561 gaaatccagc gctcaacgag cttggtaact ccaagggaga gcgctttttc gactccggca
     4621 gtcgagacca gcgtttttgt acgtccgatg tggatgagca ttcttcctcc gtctgtgcga
     4681 ctaatatcct tgacgcgaat gcgggcaatt tcggcgatac ggagaagtgt gttgtaagca
     4741 attccgagga aggcgaggtt tcggatgtcc tggcaacgat cggaattttc cataagagaa
     4801 cggacctgat cgaagtcggt tctgaaaatt tcgtcaagtc ttaggattga atacattaat
     4861 atttaaactt acctttcgaa ggcgagggct tgcttggctc tctctccagc atcaacattt
     4921 tccttgcgga tacgtctcat tacgagagat acggcattag aatctgatgg gcgtggaagt
     4981 ccagatcggc gatggagcat attgagctgt ccgagatgtt gttggatcgt cttcaccgcc
     5041 aatccacgag cttggaggta gagcaagtag tcacgaacgt cttctggctc ggctgggaac
     5101 cacttgcggt tgttaagctt gcaccaggca gcccaagaac ggcacactga gagaagcatc
     5161 ttccaggtgt gctcggagaa ggcttgtctg tcacggaaca tatccatgag gttcttgcga
     5221 acttcatcac ttgtcgcgtc aactggaagt gctggaagat tttgatgaac agtgagaaga
     5281 tttgacatat tttcgaagtt ttttagatgc actagaacaa agcgtgttgg cttcctctga
     5341 gcccgctttc cttatatacc cgcattctgc agccttacag aatgttctag aaggtcctag
     5401 atgcattcgt ttgaaaatac tcccggtggg tgcaaagaga cgcagacgga aaatgtatct
     5461 gggtctcttt attgtgtaca ctacttttcc atgtaccgaa tgtgagtcgc cctccttttg
     5521 caacaagcag ctcgaatgtt ctagaaaaag gtggaaaata gtataaatac cgttgaaaat
     5581 aaataccgaa caacatttgc tctaattgtg aaattagaaa tcttcaaact ataatcatgt
     5641 cactttacca ctatttccgt ccagctcaac gttccgtttt tggtgatcat ttttgctttc
     5701 gtcgtaaatc tacacacgcg tctcttccgt gcgagagtcc aagccagcag ccaaattcgt
     5761 tgactgagta ttcaacgttt atacgttgtc ggcaacgaga aataggaaaa tgcatcggga
     5821 aatgttcttt tttcgatttt ttccaaggtt ttgacaaatt ttaccacgaa ttttgctatg
     5881 ttttcaatta aaaaatatgt tattcaactg tttctatgag gaaaataagg ctttgcatgt
     5941 aattttctta ttcagcataa tttttaatta atttgaattt tctgtcctaa cgtttatttt
     6001 gttttcttgg ttatgactga tctgaaatta atttttgaat tttaaggtaa tatgtcaggc
     6061 ggtgccgcaa gtttgtacaa aaaagcaggc tccatgaaaa agcctgaact caccgcgacg
     6121 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     6181 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     6241 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     6301 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     6361 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     6421 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     6481 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     6541 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     6601 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     6661 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     6721 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     6781 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     6841 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     6901 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     6961 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     7021 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     7081 aaccgacgcc ccagcactcg tccgagggca aaggaataga cccagctttc ttgtacaaag
     7141 tgggtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta
     7201 tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca
     7261 cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa
     7321 aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc
     7381 tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa
     7441 ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa tttttaataa
     7501 cttcgtataa tgtatactat acgaagttat tttcagggag ccggctctta tccatacgat
     7561 gtcccagatt acgcttaccc atatgacgtt ccagactatg ccggaggact agttcgacct
     7621 gcagactggc tgtgtataag ggagcctgac atttagattc cccagaacat aaggttgata
     7681 gcgttcttga tatcgttctc acggtgagaa aggtcggcaa cctcctctcc gatgacggaa
     7741 actggaacgg aagccatgtc ggtggtcatc atacgccaag actcgtctcc gatgtgaaca
     7801 actgggtaga gctcacgtgg aaccttgtcg gaaagaagac gggcggaagc aagtgggata
     7861 accatacggc gtcctggggt atcgatgatg tcggattgga cgtcgacgaa aagacggtag
     7921 cgactctctc ttttataagt gtaaaccttg aattgcattt caccagcccc tgttctcgtc
     7981 ggcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc
     8041 cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccga
     8101 accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc
     8161 actgtaatac gctgcttcat agcatacctc tttttgacat acttcgggtt cctaggaaat
     8221 cgataccggt aacatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8281 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8341 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8401 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8461 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8521 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8581 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8641 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8701 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8761 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8821 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8881 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8941 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9001 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9061 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9121 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9181 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9241 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9301 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9361 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9421 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9481 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9541 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9601 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9661 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9721 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9781 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9841 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9901 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9961 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10021 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10081 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10141 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10201 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10261 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10321 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10381 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    10441 tcacgaggcc ctttcgtc
//
