LOCUS       sequence-335478-       15539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology 
            arms for insertion in ttTi4348 site on LGI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15539)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 15539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pTD75
FEATURES             Location/Qualifiers
     source          1..15539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1624..1657)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1657..1668)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(3090..3413)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5331..5355
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5358..6383
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5358..6383
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6384..6408)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6761..6794)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     7163..7180
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7164..7180
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             10236..10301
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             11157..11288
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             11289..11315
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     terminator      11324..11647
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(11680..11696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11680..11696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(13169..13189)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     rep_origin      complement(13326..13914)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13406..13425)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14085..14876,14877..14945))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14708..14727
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14946..15050)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     15118..15136
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(15174..15196)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     15296..15315
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    15519..15539
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 caagtgggga tcaggaagaa gttgataaag tgattcaaaa actcgaggag ctcaacacag
       61 cgatgaaccc aattttgaga gcaacactga cgaagaacta tgacgcgagg gtaggcacta
      121 ccttgaagct atccacctgc ctgcctgccg cctatttcca tgtgcagtag gaacgtgtgt
      181 aggtataggc aggcattatc gcgtctcctt tcgaactacc tagtacctag taggtagttt
      241 gaagggaggc gcgataatgc ctgcctatgt ctacacatgt gcctactgcg catggaaata
      301 ggcggtagtc aggcaggtag atagcctcat aaaattcaat acttcctttt cagaaagaat
      361 gctggaatgt attcaaagag gagtcactct tcccattgct cagagaatat gaagagttgc
      421 tcggcgagca aaagttcatg attggagatc gatattcatg ggctgacatc gctgtcgtcg
      481 agtttctaac tcggtgtcag cagtgctacg atagcttcta tctggctcat ttcgagaggc
      541 tccgaacttt ttgccagact tttgagtctc ttccgcacat tcgtccctac attcagggac
      601 gtgttgattc atttatctaa ttttttaaaa tggatatttt tcgtttttta tttgtttgtt
      661 ttgctgataa ttcaataaag ttacgcaacg aattttagac atttatgaaa accgaaaatc
      721 gacaaaaata ttttaagaaa aatagcagca aaattcaata ttttatcaaa ttttgtttat
      781 ggtaatgaga aagaaaacat acaatatttt tctaagaaga agaaaaatga ccagaatttt
      841 aagatttttc tcgaatatac gaaaaattga taaactaaaa ttttcgattt ttccatttat
      901 tttgaaaaaa aaactcttga cggttttcac catgcgaaga ggtgagaaat attctgaaag
      961 ttatgcattt tgaaaaaatt gcaaaaaaat acgttaaaat tttttgatct attgtttttt
     1021 tttgaaaacc ggaatttaaa atggaatttt ttttcaaaaa ttaaaattaa aatttaaata
     1081 tttctagtgt ttggatagtt tttcaaaagc tagatttaac ccgcgagatg tcgaccgcta
     1141 gtgtagctta cagatggctg aagacttcgc aaaaatctta tcttagaaac ccgcgaggtg
     1201 tcggccgcta aaactttgta aacctgccaa caaaatgctt cgcattgtct gaacagatga
     1261 aaccttgaaa caaaacatat acaattgaaa tcccgtaaag tgtcgggcgc aagaactgcg
     1321 attagttgta taattggact agctcaaatt tgaccaaaat ctcattttta gacctctaag
     1381 atgtcggcca ctataacttt attcaacttg aaactgcaaa aaaacttatc aactgtatag
     1441 attcaagtat tatgtcaagt attggtttta gagctacagt acactctgat aacaaaatct
     1501 gcagaaatac ctccctgtca attcccaaaa tacctcccct catctcaatt atcccgaaaa
     1561 tcggttagtc attgccatca gaaaatcgaa ttaggtacca tgatggtagc aaactcactt
     1621 cgtataactt cgtatagtat accttatacg aagttatctg ccctcgattg tctatcttcc
     1681 attctcacca agtttcaaca actttattca gtcattcttc tctggttcat ttcccaacca
     1741 atcttcccga ataaagccca tctgtccttt cggccacatt cctcacctac atccgtactc
     1801 cttatctccc gttcttatcc agtcttttat cgtctctgcg tctcgcacac tgtctcgtcg
     1861 gtaatgtaaa aaaaaaacaa aaaaaacttg aaaacttgcg gaaagatttc ctaataaaag
     1921 gagacgaatc cggtaggaaa tcgacaagtc tctgcttctc gaagactagg tgagttacat
     1981 ttctttggcc gaattatttt gaaatcctaa atttgataat ttcagagatg tctgtaaaac
     2041 ttgcgtgtta tgtgacggct tcggtcactg tcgccactct tatggtatgc ttcatgacca
     2101 tgtcaaccat ctactcggag gttgatggat tcagagagaa gcttgatacc gagatgaatg
     2161 tgttcagagt gagttaaatt atattttgaa ttttaaataa tttttaattt tctagcaatc
     2221 taccaatgga ttgtggaagg acatagttgt catcggaaga tctagcaagc gtgtccgttg
     2281 tcaatatgaa gagaccaacg ctaccccaac tccacatgct gatggatccc catctgctcc
     2341 accaggtcaa ccaccagcag ttccaccagt cttcaaccag ccaaagactc caaatggagc
     2401 caatggaaat ggaccaacct gcaactgcaa tgctgataac aagtgcccag ctggaccatc
     2461 cggaccaaag ggagttccag gagttccagg actcgacgga gttccaggac ttgacggtgt
     2521 tccaggagtt ggagctgatg atatcgctcc acaacgcgag tctgtcggat gcttcacttg
     2581 cccacaagga ccagttggac caccaggagc tcttggaaga ccaggaccac gtggacttcc
     2641 aggaccaaga ggacaaaatg gaaacccagg aagagatgga caaccaggac atccaggaga
     2701 gcaaggatca tccggtcaaa tcggaaagat cggagagcca ggaccaccag gagagaaggg
     2761 acgcgacgcc gagcatccaa tcggaagacc aggaccaaag ggaccaagag gagatcaagg
     2821 accaacagga ccagctggac agaacggtct tcacggacca ccaggagagc caggaaccgt
     2881 tggaccagaa ggaccatctg gaaagcaagg acgtcaagga ccagacggaa cccagggaga
     2941 gactggacca gacggaagac caggaaagga tgccgagtac tgccagtgcc cagacaagtc
     3001 tccaccatca gaggctgtca acgccaaccg tggatacaga aatatctaaa ttgttggtgt
     3061 tttctaataa aatatttgag attcagtgag acttttttct tggcggcaca ataaagtttc
     3121 ctctttttgt tgggtcactc aacgaacaca atacaaatat ttgagcgctc gcatttttct
     3181 caacctttcg ggtttaaagc acttcaaaat ggaattgaag agagattgtg aaagtacatc
     3241 agacagaatc acattttgat agcattcact tcactcagat gcaaactttt cagtgactat
     3301 aaaaagtcat aaaataataa cattaaaaaa gcaaataaat tagcgagaga attttttgac
     3361 aaaaagaaag aagagtgata gagaagaagg gaatgcttga aaggattttg catttatctt
     3421 aatcaccgtc ttcgagaaga cgaaccatgg cgccagtttc agaatccaag tttctaatat
     3481 agttcatcac aatgttgacg ttggtccaac ctccggcttg cataatctct gggatggaaa
     3541 ctccagcgcg agccatgtca cgggcagctc cgacacgggc ggaatgtcca gaccaagcca
     3601 agtaacgttg tccggaatca tccttggctc cgtagatgag acggtgggta gcttcgaaga
     3661 ttcctgaaaa tttattttca gcttattctt agctaatatg ttgtttaaac ttaccttcaa
     3721 gagctcgagt cgagagttgt gatgttgcac tcggggcagc aactccgttc ttgcgaacac
     3781 gacagaacag gtagttgttt ggatcatcgg cgactccgga aaccgaaatc cagcgctcaa
     3841 cgagcttggt aactccaagg gagagcgctt tttcgactcc ggcagtcgag accagcgttt
     3901 ttgtacgtcc gatgtggatg agcattcttc ctccgtctgt gcgactaata tccttgacgc
     3961 gaatgcgggc aatttcggcg atacggagaa gtgtgttgta agcaattccg aggaaggcga
     4021 ggtttcggat gtcctggcaa cgatcggaat tttccataag agaacggacc tgatcgaagt
     4081 cggttctgaa aatttcgtca agtcttagga ttgaatacat taatatttaa acttaccttt
     4141 cgaaggcgag ggcttgcttg gctctctctc cagcatcaac attttccttg cggatacgtc
     4201 tcattacgag agatacggca ttagaatctg atgggcgtgg aagtccagat cggcgatgga
     4261 gcatattgag ctgtccgaga tgttgttgga tcgtcttcac cgccaatcca cgagcttgga
     4321 ggtagagcaa gtagtcacga acgtcttctg gctcggctgg gaaccacttg cggttgttaa
     4381 gcttgcacca ggcagcccaa gaacggcaca ctgagagaag catcttccag gtgtgctcgg
     4441 agaaggcttg tctgtcacgg aacatatcca tgaggttctt gcgaacttca tcacttgtcg
     4501 cgtcaactgg aagtgctgga agattttgat gaacagtgag aagatttgac atattttcga
     4561 agttttttag atgcactaga acaaagcgtg ttggcttcct ctgagcccgc tttccttata
     4621 tacccgcatt ctgcagcctt acagaatgtt ctagaaggtc ctagatgcat tcgtttgaaa
     4681 atactcccgg tgggtgcaaa gagacgcaga cggaaaatgt atctgggtct ctttattgtg
     4741 tacactactt ttccatgtac cgaatgtgag tcgccctcct tttgcaacaa gcagctcgaa
     4801 tgttctagaa aaaggtggaa aatagtataa ataccgttga aaataaatac cgaacaacat
     4861 ttgctctaat tgtgaaatta gaaatcttca aactataatc atgtcacttt accactattt
     4921 ccgtccagct caacgttccg tttttggtga tcatttttgc tttcgtcgta aatctacaca
     4981 cgcgtctctt ccgtgcgaga gtccaagcca gcagccaaat tcgttgactg agtattcaac
     5041 gtttatacgt tgtcggcaac gagaaatagg aaaatgcatc gggaaatgtt cttttttcga
     5101 ttttttccaa ggttttgaca aattttacca cgaattttgc tatgttttca attaaaaaat
     5161 atgttattca actgtttcta tgaggaaaat aaggctttgc atgtaatttt cttattcagc
     5221 ataattttta attaatttga attttctgtc ctaacgttta ttttgttttc ttggttatga
     5281 ctgatctgaa attaattttt gaattttaag gtaatatgtc aggcggtgcc gcaagtttgt
     5341 acaaaaaagc aggctccatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     5401 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     5461 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     5521 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     5581 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     5641 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     5701 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     5761 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     5821 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     5881 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     5941 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     6001 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     6061 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     6121 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     6181 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     6241 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     6301 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     6361 ctcgtccgag ggcaaaggaa tagacccagc tttcttgtac aaagtgggtc caattactct
     6421 tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa
     6481 aaaacttctc ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat
     6541 tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct
     6601 ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat
     6661 gttttttact tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc
     6721 ttatcatatg ttacgtttca gtttatgacc gcaattttta ataacttcgt atagtatacc
     6781 ttatacgaag ttatactagt atatattttt atgttttctt ttaaaccttc ctccatattt
     6841 ctgaattgaa tgttgttgca tattgtgtgt ccttatttca tttccatcat ccttttgtgt
     6901 gtttccccct tccaccaatt ccccccacgt cctaatgaaa tgaaagcgag aagataaata
     6961 aacttgcaca ttgtgttcag atgagaggag cggaatacga attgggagac ggaaagagaa
     7021 aaaaaaatgt ttagatttgg attgaattaa ttttttagaa agcaagaaaa aaaacacaaa
     7081 gagaataaat aaataatagg aaaaaaaaca aatattcagt atttaaaatt tgaaaattca
     7141 acgacgttgg cgtcgatcat cctgtaaaac gacggccagt gcatgcgatc ttcatccctt
     7201 caacatttgg cagaaaagtg caattcgtct tttacaggaa ttcgatttcc gatctcttgc
     7261 acaactcgag gcgtcactcc agatggatta ggaactgttt ccctttgtag tacatgttgg
     7321 gtgtggcgac gtcttccgag tacctactat cctgcatatc ttaacgaagt cgtgtgcgat
     7381 tatgcggaca catcgtgtct gagtggtagt gaaaagacag agaaagtggg ggaaaaagaa
     7441 aataccattt tttcaggata cgcgtcatgc caaactggaa cccaacagtt gaatgtgcta
     7501 cgaaatgaca gcgggaaatt gattccaata tcggtatcag ctggaatcaa ttgtgaatgt
     7561 agattagcag ttggatcaac acttgaatca ctcgtacttg gacaaggaat ttccaaagca
     7621 atgccgccaa ttgacacaac tagtacaaaa ccaccaaatt tggcaacaag cacaacaagc
     7681 cattcctaat ttatttgggt attttactct tgataattgt attaatgatc atttaatgtt
     7741 gttgaatatc acacatttaa catattcaat cgaagaaata taacgacgtt ccacaacatc
     7801 cagtatacgt cttcctctgt attgagtttt attctttgac attgtgtcat gtttcagaac
     7861 atagaaatat accaaagtgt tgagaagcat tgtataattc aattgaatct ttttcagttt
     7921 acctcttctc ctttttcacc gtttccgtca tcaaaacgcc gttattcaag gtgagattat
     7981 ggcgataaat gaatgccatt cacaaaattg caagacacta ttaaagaaga agaggtttag
     8041 agatttgtac cgatcatagc ttcttctgag aaagcgacct tggcggccgt acttgaagag
     8101 aagcaatttt tatcgccgta gaagatgttg cctgccaccc accctgtacg ttgttttttt
     8161 gcttatcaac atctgaattt tgttgtcttt tgcaccgcnt acttttacta tggaatttga
     8221 ggaaatataa tatgaattga agagaatacg ctacctgtca aagaaacgtc acggttaggg
     8281 tagtataaaa gggaagggta acactgaata aggtacctgg cttactttca gtcatgcaaa
     8341 agagaatcgc cttgtcgttt ccagaagaag tactcgagca cgtcttctcc ttcatccaac
     8401 tcgacaagga ccgtaactcc gtctccctcg tctgcaagtc ctggtacgag atcgagcgtt
     8461 ggtgccgtcg taaggtcttc atcggaaact gctacgccgt ctccccagcc accgtcatcc
     8521 gtcgtttccc aaaggtccgt tccgtcgagc tcaagggaaa gccacacttc gccgacttca
     8581 acctcgtccc agacggatgg ggaggatacg tctacccatg gatcgaggcc atgtcctcct
     8641 cctacacctg gctcgaggag atccgtctca agcgtatggt cgtcaccgac gactgcctcg
     8701 agctcatcgc caagtccttc aagaacttca aggtcctcgt cctctcctcc tgcgagggat
     8761 tctccaccga cggactcgcc gccatcgccg ccacctgccg taacctcaag gtaagtttaa
     8821 acatatatat actaactaac cctgattatt taaattttca ggagctcgac ctccgtgagt
     8881 ccgacgtcga cgaggtctcc ggacactggc tctcccactt cccagacacc tacacctccc
     8941 tcgtctccct caacatctcc tgcctcgcct ccgaggtctc cttctccgcc ctcgagcgtc
     9001 tcgtcacccg ttgcccaaac ctcaagtccc tcaagctcaa ccgtgccgtc ccactcgaga
     9061 agctcgccac cctcctccaa cgtgccccac aactcgagga gctcggaacc ggaggataca
     9121 ccgccgaggt ccgtccagac gtctactccg gactctccgt cgccctctcc ggatgcaagg
     9181 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag gagctccgtt
     9241 gcctctccgg attctgggac gccgtcccag cctacctccc agccgtctac tccgtctgct
     9301 cccgtctcac caccctcaac ctctcctacg ccaccgtcca atcctacgac ctcgtcaagc
     9361 tcctctgcca atgcccaaag ctccaacgtc tctgggtcct cgactacatc gaggacgccg
     9421 gactcgaggt cctcgcctcc acctgcaagg acctccgtga gctccgtgtc ttcccatccg
     9481 agccattcgt catggagcca aacgtcgccc tcaccgagca aggactcgtc tccgtctcca
     9541 tgggatgccc aaagctcgag tccgtcctct acttctgccg tcaaatgacc aacgccgccc
     9601 tcatcaccat cgcccgtaac cgtccaaaca tgacccgttt ccgtctctgc atcatcgagc
     9661 caaaggcccc agactacctc accctcgagc cactcgacat cggattcgga gccatcgtcg
     9721 agcactgcaa ggacctccgt cgtctctccc tctccggact cctcaccgac aaggtcttcg
     9781 agtacatcgg aacctacgcc aagaagatgg agatgctctc cgtcgccttc gccggagact
     9841 ccgacctcgg actccaccac gtcctctccg gatgcgactc cctccgtaag ctcgagatcc
     9901 gtgactgccc attcggagac aaggccctcc tcgccaacgc ctccaagctc gagaccatgc
     9961 gttccctctg gatgtcctcc tgctccgtct ccttcggagc ctgcaagctc ctcggacaaa
    10021 agatgccaaa gctcaacgtc gaggtcatcg acgagcgtgg agccccagac tcccgtccag
    10081 agtcctgccc agtcgagcgt gtcttcatct accgtaccgt cgccggacca cgtttcgaca
    10141 tgccaggatt cgtctggaac atggaccaag actccaccat gcgtttctcc cgtcaaatca
    10201 tcaccaccaa cggactcgga ggaggaggat ccccagtcaa gcaaaccctc aacttcgacc
    10261 tcctcaagct cgccggagac gtcgagtcca acccaggacc aatggtttcg gaattgatta
    10321 aggaaaatat gcacatgaag ctctacatgg agggaaccgt cgacaaccac cacttcaagt
    10381 gcacctccga gggagaggga aagccatacg agggaaccca aaccatgcgt atcaaggtcg
    10441 tcgagggagg accactccca ttcgccttcg acatcctcgc cacctccttc ctctacggat
    10501 ccaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagacct
    10561 tcatcaacca cacccaagga atcccagact tcttcaagca atccttccca gagggattca
    10621 cctgggagcg tgtcaccacc tacgaggacg gaggagtcct caccgccacc caagacacct
    10681 ccctccaaga cggatgcctc atctacaacg tcaaggtaag tttaaacagt tcggtactaa
    10741 ctaaccatac atatttaaat tttcagatcc gtggagtcaa cttcacctcc aacggaccag
    10801 tcatgcaaaa gaagaccctc ggatgggagg ccttcaccga gaccctctac ccagccgacg
    10861 gaggactcga gggacgtaac gacatggccc tcaagctcgt cggaggatcc cacctcatcg
    10921 ccaacgccaa ggtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc
    10981 agaccaccta ccgttccaag aagccagcca agaacctcaa gatgccagga gtctactacg
    11041 tcgactaccg tctcgagcgt atcaaggagg ccaacaacga gacctacgtc gagcaacacg
    11101 aggtcgccgt cgcccgttac tgcgacctcc catccaagct cggacacaag ctcaacccta
    11161 aagatccagc caaacctccg gccaaggcac aagttgtggg atggccaccg gtgagatcat
    11221 accggaagaa cgtgatggtt tcctgccaaa aatcaagcgg tggcccggag gcggcggcgt
    11281 tcgtgaaacc agccgccaag cgtgtcaagc tcgactaaga taaatgcaaa atcctttcaa
    11341 gcattccctt cttctctatc actcttcttt ctttttgtca aaaaattctc tcgctaattt
    11401 atttgctttt ttaatgttat tattttatga ctttttatag tcactgaaaa gtttgcatct
    11461 gagtgaagtg aatgctatca aaatgtgatt ctgtctgatg tactttcaca atctctcttc
    11521 aattccattt tgaagtgctt taaacccgaa aggttgagaa aaatgcgagc gctcaaatat
    11581 ttgtattgtg ttcgttgagt gacccaacaa aaagaggaaa ctttattgtg ccgccaagaa
    11641 aaaagtctca cgtacggtgc gcgcgatgcc ctaggcatgg tcatagctgt ttcctgcgca
    11701 agtcggcgat ttctttgaag ttttgtcaaa agaagagaca aggacttgat agatacgctc
    11761 atcagagtga gattcattgc aaatagtgtg taaatcgtgg agagacgaga gaaaaggtgt
    11821 gagaagctta gaagataaag aataggaaga agaacatatc tcatttccta ttttctctaa
    11881 tttttcgttc atttttctag acattaccca tttgatggtt tttcaatatg gttatcaggg
    11941 tactcatttt ggtagttttc aaagcaattt ggagtttttt tttaaattgt caactacaaa
    12001 aacgcagctt aaaggtccaa atatcatagt ataaaaattc tttaaaaaat tccaaatttt
    12061 atatgatttt ttgaaaattg gaagaatgtt agtttttgcc atttttttgg aagtttttct
    12121 ccggatttta tggtacttat atttcaaata taaatttttg taggaattcg agtatgcgag
    12181 agagctatag tttactaaaa aaaagtggca aaaactcagt aattgccact tttttcaaaa
    12241 atgttttgaa aaattttact atgatatttg gccattttgg caccatatgt acgttacttt
    12301 ttacgaaaca ctctaatttt tgaaatttga gtttcatgtt taatcttttg tataaaattt
    12361 ttatggtcct tttgtgacag gataaaaaat tgcaaaaaaa tagaaaactt tgtttcgtga
    12421 aaacacctag tttaaaaatt gtgggccact tagcgattct agtcaaaaat cagacaaatg
    12481 tcagctgccg taactttttc cggatcaggg ttatcaaatt ttaattttaa ttgtaaatgt
    12541 agaaaaatga tcggcaacat tttgctaatt gaaaatattt tgaaaattgc tatagtacat
    12601 attaatctag tattaatata gtattaatgt tataatatca gttatgtgtt tgaataactc
    12661 aagcgtaagt ttccgtaaaa tatctttaaa ttttggtaat tttttacggt ttggtataaa
    12721 tttttgcatt ttttgatttt ctacactttt tttcgatttc gcattttccg aatttctctg
    12781 aaataaatcg aacaatcgga aaattttcga gggcaccctt agccatgcag ctcaggaatt
    12841 tgactttatg ttcccattca aattttttta aaattaaata aaatgctaat tgtaagataa
    12901 attacaaaaa ttattaaatc tcatcatcct tatgatgata gtgcagtgca tgaatatatt
    12961 tttccaaata ggatatgatt cgtcgagtag cttcacgtaa tccgttttct tcaatcacca
    13021 tttcgaactg caattttttt tttggatttt tgtaacaata ttgtcataaa ttgtgatttt
    13081 gatattttgc gtagagcatc agaacctttc tgctttgaat tttaatggga ttaaaaatta
    13141 taaaaaactt gcaccctaat taaagcgcca actttattat acatagttga taattcgatg
    13201 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    13261 aagaacatgt gagcaaaagg ccagcaaaag accaggaacc gtaaaaaggc cgcgttgctg
    13321 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    13381 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    13441 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    13501 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    13561 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    13621 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    13681 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    13741 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
    13801 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    13861 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    13921 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    13981 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    14041 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    14101 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    14161 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    14221 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    14281 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    14341 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    14401 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    14461 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    14521 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    14581 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    14641 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    14701 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    14761 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    14821 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    14881 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    14941 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    15001 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    15061 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
    15121 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
    15181 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    15241 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
    15301 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
    15361 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
    15421 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    15481 cgattaagtt gggtaacgcc agggatccgc caagctatca actttgtata gaaaagttg
//
