LOCUS       sequence-335479-       17730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal promoter rgef-1P::TIR1::F2A::TagBFP2::AID* with homology 
            arms for insertion in ttTi4348 site on chromosome I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17730)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 17730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pTD79
FEATURES             Location/Qualifiers
     source          1..17730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1624..1657)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1657..1668)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(3090..3413)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5331..5355
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5358..6383
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5358..6383
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6384..6408)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6761..6794)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     7163..7180
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7164..7180
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             12427..12492
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             13348..13479
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             13480..13506
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     terminator      13515..13838
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(13871..13887)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13871..13887)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(15360..15380)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     rep_origin      complement(15517..16105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15597..15616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16276..17067,17068..17136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16899..16918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17137..17241)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     17309..17327
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(17365..17387)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     17487..17506
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    17710..17730
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 caagtgggga tcaggaagaa gttgataaag tgattcaaaa actcgaggag ctcaacacag
       61 cgatgaaccc aattttgaga gcaacactga cgaagaacta tgacgcgagg gtaggcacta
      121 ccttgaagct atccacctgc ctgcctgccg cctatttcca tgtgcagtag gaacgtgtgt
      181 aggtataggc aggcattatc gcgtctcctt tcgaactacc tagtacctag taggtagttt
      241 gaagggaggc gcgataatgc ctgcctatgt ctacacatgt gcctactgcg catggaaata
      301 ggcggtagtc aggcaggtag atagcctcat aaaattcaat acttcctttt cagaaagaat
      361 gctggaatgt attcaaagag gagtcactct tcccattgct cagagaatat gaagagttgc
      421 tcggcgagca aaagttcatg attggagatc gatattcatg ggctgacatc gctgtcgtcg
      481 agtttctaac tcggtgtcag cagtgctacg atagcttcta tctggctcat ttcgagaggc
      541 tccgaacttt ttgccagact tttgagtctc ttccgcacat tcgtccctac attcagggac
      601 gtgttgattc atttatctaa ttttttaaaa tggatatttt tcgtttttta tttgtttgtt
      661 ttgctgataa ttcaataaag ttacgcaacg aattttagac atttatgaaa accgaaaatc
      721 gacaaaaata ttttaagaaa aatagcagca aaattcaata ttttatcaaa ttttgtttat
      781 ggtaatgaga aagaaaacat acaatatttt tctaagaaga agaaaaatga ccagaatttt
      841 aagatttttc tcgaatatac gaaaaattga taaactaaaa ttttcgattt ttccatttat
      901 tttgaaaaaa aaactcttga cggttttcac catgcgaaga ggtgagaaat attctgaaag
      961 ttatgcattt tgaaaaaatt gcaaaaaaat acgttaaaat tttttgatct attgtttttt
     1021 tttgaaaacc ggaatttaaa atggaatttt ttttcaaaaa ttaaaattaa aatttaaata
     1081 tttctagtgt ttggatagtt tttcaaaagc tagatttaac ccgcgagatg tcgaccgcta
     1141 gtgtagctta cagatggctg aagacttcgc aaaaatctta tcttagaaac ccgcgaggtg
     1201 tcggccgcta aaactttgta aacctgccaa caaaatgctt cgcattgtct gaacagatga
     1261 aaccttgaaa caaaacatat acaattgaaa tcccgtaaag tgtcgggcgc aagaactgcg
     1321 attagttgta taattggact agctcaaatt tgaccaaaat ctcattttta gacctctaag
     1381 atgtcggcca ctataacttt attcaacttg aaactgcaaa aaaacttatc aactgtatag
     1441 attcaagtat tatgtcaagt attggtttta gagctacagt acactctgat aacaaaatct
     1501 gcagaaatac ctccctgtca attcccaaaa tacctcccct catctcaatt atcccgaaaa
     1561 tcggttagtc attgccatca gaaaatcgaa ttaggtacca tgatggtagc aaactcactt
     1621 cgtataactt cgtatagtat accttatacg aagttatctg ccctcgattg tctatcttcc
     1681 attctcacca agtttcaaca actttattca gtcattcttc tctggttcat ttcccaacca
     1741 atcttcccga ataaagccca tctgtccttt cggccacatt cctcacctac atccgtactc
     1801 cttatctccc gttcttatcc agtcttttat cgtctctgcg tctcgcacac tgtctcgtcg
     1861 gtaatgtaaa aaaaaaacaa aaaaaacttg aaaacttgcg gaaagatttc ctaataaaag
     1921 gagacgaatc cggtaggaaa tcgacaagtc tctgcttctc gaagactagg tgagttacat
     1981 ttctttggcc gaattatttt gaaatcctaa atttgataat ttcagagatg tctgtaaaac
     2041 ttgcgtgtta tgtgacggct tcggtcactg tcgccactct tatggtatgc ttcatgacca
     2101 tgtcaaccat ctactcggag gttgatggat tcagagagaa gcttgatacc gagatgaatg
     2161 tgttcagagt gagttaaatt atattttgaa ttttaaataa tttttaattt tctagcaatc
     2221 taccaatgga ttgtggaagg acatagttgt catcggaaga tctagcaagc gtgtccgttg
     2281 tcaatatgaa gagaccaacg ctaccccaac tccacatgct gatggatccc catctgctcc
     2341 accaggtcaa ccaccagcag ttccaccagt cttcaaccag ccaaagactc caaatggagc
     2401 caatggaaat ggaccaacct gcaactgcaa tgctgataac aagtgcccag ctggaccatc
     2461 cggaccaaag ggagttccag gagttccagg actcgacgga gttccaggac ttgacggtgt
     2521 tccaggagtt ggagctgatg atatcgctcc acaacgcgag tctgtcggat gcttcacttg
     2581 cccacaagga ccagttggac caccaggagc tcttggaaga ccaggaccac gtggacttcc
     2641 aggaccaaga ggacaaaatg gaaacccagg aagagatgga caaccaggac atccaggaga
     2701 gcaaggatca tccggtcaaa tcggaaagat cggagagcca ggaccaccag gagagaaggg
     2761 acgcgacgcc gagcatccaa tcggaagacc aggaccaaag ggaccaagag gagatcaagg
     2821 accaacagga ccagctggac agaacggtct tcacggacca ccaggagagc caggaaccgt
     2881 tggaccagaa ggaccatctg gaaagcaagg acgtcaagga ccagacggaa cccagggaga
     2941 gactggacca gacggaagac caggaaagga tgccgagtac tgccagtgcc cagacaagtc
     3001 tccaccatca gaggctgtca acgccaaccg tggatacaga aatatctaaa ttgttggtgt
     3061 tttctaataa aatatttgag attcagtgag acttttttct tggcggcaca ataaagtttc
     3121 ctctttttgt tgggtcactc aacgaacaca atacaaatat ttgagcgctc gcatttttct
     3181 caacctttcg ggtttaaagc acttcaaaat ggaattgaag agagattgtg aaagtacatc
     3241 agacagaatc acattttgat agcattcact tcactcagat gcaaactttt cagtgactat
     3301 aaaaagtcat aaaataataa cattaaaaaa gcaaataaat tagcgagaga attttttgac
     3361 aaaaagaaag aagagtgata gagaagaagg gaatgcttga aaggattttg catttatctt
     3421 aatcaccgtc ttcgagaaga cgaaccatgg cgccagtttc agaatccaag tttctaatat
     3481 agttcatcac aatgttgacg ttggtccaac ctccggcttg cataatctct gggatggaaa
     3541 ctccagcgcg agccatgtca cgggcagctc cgacacgggc ggaatgtcca gaccaagcca
     3601 agtaacgttg tccggaatca tccttggctc cgtagatgag acggtgggta gcttcgaaga
     3661 ttcctgaaaa tttattttca gcttattctt agctaatatg ttgtttaaac ttaccttcaa
     3721 gagctcgagt cgagagttgt gatgttgcac tcggggcagc aactccgttc ttgcgaacac
     3781 gacagaacag gtagttgttt ggatcatcgg cgactccgga aaccgaaatc cagcgctcaa
     3841 cgagcttggt aactccaagg gagagcgctt tttcgactcc ggcagtcgag accagcgttt
     3901 ttgtacgtcc gatgtggatg agcattcttc ctccgtctgt gcgactaata tccttgacgc
     3961 gaatgcgggc aatttcggcg atacggagaa gtgtgttgta agcaattccg aggaaggcga
     4021 ggtttcggat gtcctggcaa cgatcggaat tttccataag agaacggacc tgatcgaagt
     4081 cggttctgaa aatttcgtca agtcttagga ttgaatacat taatatttaa acttaccttt
     4141 cgaaggcgag ggcttgcttg gctctctctc cagcatcaac attttccttg cggatacgtc
     4201 tcattacgag agatacggca ttagaatctg atgggcgtgg aagtccagat cggcgatgga
     4261 gcatattgag ctgtccgaga tgttgttgga tcgtcttcac cgccaatcca cgagcttgga
     4321 ggtagagcaa gtagtcacga acgtcttctg gctcggctgg gaaccacttg cggttgttaa
     4381 gcttgcacca ggcagcccaa gaacggcaca ctgagagaag catcttccag gtgtgctcgg
     4441 agaaggcttg tctgtcacgg aacatatcca tgaggttctt gcgaacttca tcacttgtcg
     4501 cgtcaactgg aagtgctgga agattttgat gaacagtgag aagatttgac atattttcga
     4561 agttttttag atgcactaga acaaagcgtg ttggcttcct ctgagcccgc tttccttata
     4621 tacccgcatt ctgcagcctt acagaatgtt ctagaaggtc ctagatgcat tcgtttgaaa
     4681 atactcccgg tgggtgcaaa gagacgcaga cggaaaatgt atctgggtct ctttattgtg
     4741 tacactactt ttccatgtac cgaatgtgag tcgccctcct tttgcaacaa gcagctcgaa
     4801 tgttctagaa aaaggtggaa aatagtataa ataccgttga aaataaatac cgaacaacat
     4861 ttgctctaat tgtgaaatta gaaatcttca aactataatc atgtcacttt accactattt
     4921 ccgtccagct caacgttccg tttttggtga tcatttttgc tttcgtcgta aatctacaca
     4981 cgcgtctctt ccgtgcgaga gtccaagcca gcagccaaat tcgttgactg agtattcaac
     5041 gtttatacgt tgtcggcaac gagaaatagg aaaatgcatc gggaaatgtt cttttttcga
     5101 ttttttccaa ggttttgaca aattttacca cgaattttgc tatgttttca attaaaaaat
     5161 atgttattca actgtttcta tgaggaaaat aaggctttgc atgtaatttt cttattcagc
     5221 ataattttta attaatttga attttctgtc ctaacgttta ttttgttttc ttggttatga
     5281 ctgatctgaa attaattttt gaattttaag gtaatatgtc aggcggtgcc gcaagtttgt
     5341 acaaaaaagc aggctccatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     5401 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     5461 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     5521 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     5581 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     5641 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     5701 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     5761 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     5821 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     5881 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     5941 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     6001 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     6061 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     6121 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     6181 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     6241 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     6301 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     6361 ctcgtccgag ggcaaaggaa tagacccagc tttcttgtac aaagtgggtc caattactct
     6421 tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa
     6481 aaaacttctc ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat
     6541 tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct
     6601 ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat
     6661 gttttttact tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc
     6721 ttatcatatg ttacgtttca gtttatgacc gcaattttta ataacttcgt atagtatacc
     6781 ttatacgaag ttatactagt atatattttt atgttttctt ttaaaccttc ctccatattt
     6841 ctgaattgaa tgttgttgca tattgtgtgt ccttatttca tttccatcat ccttttgtgt
     6901 gtttccccct tccaccaatt ccccccacgt cctaatgaaa tgaaagcgag aagataaata
     6961 aacttgcaca ttgtgttcag atgagaggag cggaatacga attgggagac ggaaagagaa
     7021 aaaaaaatgt ttagatttgg attgaattaa ttttttagaa agcaagaaaa aaaacacaaa
     7081 gagaataaat aaataatagg aaaaaaaaca aatattcagt atttaaaatt tgaaaattca
     7141 acgacgttgg cgtcgatcat cctgtaaaac gacggccagt gcatgcaaga ctaattttcg
     7201 attaacccgt aggggtgcaa gactaataga gactgcaaga ctattagagg ctgaaatact
     7261 aattttcgta tgctcaataa ttttggaaat tggcctattt tttgtagaaa cttgataccg
     7321 tttaaacaag gaaaaataca cactttttaa tatttcatca ataatttgaa cgattttgtg
     7381 attttaagtt caatttgccc aaaaaaagac aatttttctg acgttaccgc aaagtaatgc
     7441 tggtcaggca aaaagagcgg tgcaaaaata taagagactg caatactaat agaggaaata
     7501 cggtaattga attttagtga aacttgcgac agttttcctc atttttttgt tattccggca
     7561 tacgagtgtg gcatacgagt gaggtcatct tttgtttctt ccgtttcttc atcgcttttg
     7621 aaaaaaaatg ttgaagaaaa cttcgtagcc gatagccgga taatgtttgg cacggcgttc
     7681 caacatatat cattggaaat tttttaagat ttttgcgaaa aatcacattc ttcacgatga
     7741 gaacacgtta ttgaaggaat ataaatcaag aataacatat agttatattt ctctattact
     7801 ttaacgttaa atatgagcaa atttgagcat tttgattgcg atgaaaagca gaatcgagtc
     7861 aactgaaatc cgttcaaaaa cctaagctcg gttcgcccga aagtcgattt ttccaatagc
     7921 cgaacagcac tgcttcttta ttttccaggt atttttgggg tgtaagaatt atatcttctt
     7981 tgtttgggta tttacgtttc cgaattccct ttcttgatct gtcgctattt ttcagaaacc
     8041 agaacagttt atcctttttc atgagaacct gaatagctca aaaacccgtt taatttcttt
     8101 tcatcatctc atttgaaact ttcctagtct tcttaatgat ttccaggctc ctcctacatt
     8161 tgtatctcaa gatgcataca ctttatttcc gctccaattc gaatcatcta cttctctttt
     8221 ttcttcaact ttctactttt ttccattctt ttctttcaaa tgttcgacgt cttcaagtga
     8281 ctgctcttca ttctcctcat ttttctcgaa tttcattctt gtctcttttt tgctcaaaaa
     8341 tggaaaatga aagtgacgtg agatttggac ggcgggacac gggggcagta gaagcagcaa
     8401 aaaggagaga aagaggacac aaataagaag aacgaattca aaaataagcg gagaggagct
     8461 atttccgtca attctacctc cccaatcttc atcaattcgg ctcaattgaa tgacgtcaca
     8521 gggagataaa ccgtttggat gagcgccgtc catcactgac gccatcccgt ttgggacaag
     8581 aaaagagaaa aaagagcaca aagtttttgg tgacggatct tgtcaatcat atgaaagttg
     8641 ttctgattga ttgtcagttt ttttcctact tttttggatt ctatccactt ctgaactttt
     8701 gacaagtttc aaactttctg aatcattttc tatgcatttt cctggaattc tttttatgta
     8761 aaatatgaaa tagaatgttt ttgaattcaa gttctgcttt tttcttcttt tttgttctgt
     8821 tcgggcttgg gtatgctttt tttaaaaatt attttgcaca tcgaccaata agtgcgcaac
     8881 ttataaaatt aatttatttt tgttaatttt tgaaatactt gtattgcttt aagtgatctg
     8941 acctcgccct gagctttcca cgtagttatc aaatacaaat cctccaaggg taacgtacct
     9001 atattactga tctttataat aactttatca cctgtccagt tccagaggaa ttctgttaag
     9061 cttataggca cagaaggagt catttctgct ggtttttaat gatccaaatc tttatttcaa
     9121 gtaaaaaact gaacacttgc gaataaaact atcagattaa ccattcacca aaaatgtgtt
     9181 tgaatctaaa actttctcag tattccaaat atagaaataa ataaccacga cattgctaaa
     9241 atctgtctga attgtgtact ccttaccgtg aagtaataat ggatataatg aatcgtttga
     9301 aatgaatgat cagcacattt ttggtgaaag atcacaaata aggaataagc gacggaaaat
     9361 aaaacgatta tttcggatca aaaatttgtt gaagatcata tacactcgag accaagatta
     9421 ttctagacaa ttttcaaatt ggcttctttg tttgcaaatc ttcataataa tctgtgaagt
     9481 ttggaaattt gaatttttaa tcttttttca tagattatag tttttatttc tttgcaaaac
     9541 tatattaaaa accgatgcat tgttttaggg aaattaatga gccttttgtt caacactaaa
     9601 aacaataaaa ttaaaatttt ggcttcatca tttgaccttt tttaagttcg aaaacttttc
     9661 tcgtatttct gaaccgccaa ttttttcaca catctctaga cttttggtgc ccgttccaga
     9721 aagttaagta attgctattc taagaaagtt ctcaacattg ttttttagtt ctgattgaat
     9781 tctgatgttc caggaatata ttttaaattt aatattcttt gcactatttc tatactaact
     9841 aaataataaa tagtctaagt atgttcaatg agaacaaaaa agctctatct atatttttct
     9901 atcctatttc atttatatcc ttttcatttt gaactcacca gcttctcttc ttcttcttgt
     9961 ccatatactt cttaaaagtc tcgaacttct ctctctcact ctccatcaat ttctttatgg
    10021 ctaccgcttg cgctctgccg cttccgaaga aagaaggcgt tggcagagct ctcaattcta
    10081 ttttttctcg ccgtaggcta tcgatttctc agcttctctt ctctcttcct cactccacca
    10141 ccaccacttc gggcttcttc ttttcttcat tttcgtcttc ttcttcatcc tcttcttttt
    10201 ttttcagttc cccctcactc ctccccttat atgcgcgtgc gagaggggtg caaaagcagc
    10261 gcgcatccga gaattgaaac gagaaaacgg agacgcagca gcagttcgtc ctcagaaaaa
    10321 tagccagtaa aaagagaaaa agatagagag agacctatcg cattttattt tcaaattgca
    10381 attcctgcaa ttcatgtgtg cgtgtgccca ttttcaattc ttcccgttat ttttcagacc
    10441 attaccaacg ttctttctag atcttgagac aatcttcctt ctgctcaatc gttcgtcgtg
    10501 aagacggcat cgacgacgac gacgatgcaa aagagaatcg ccttgtcgtt tccagaagaa
    10561 gtactcgagc acgtcttctc cttcatccaa ctcgacaagg accgtaactc cgtctccctc
    10621 gtctgcaagt cctggtacga gatcgagcgt tggtgccgtc gtaaggtctt catcggaaac
    10681 tgctacgccg tctccccagc caccgtcatc cgtcgtttcc caaaggtccg ttccgtcgag
    10741 ctcaagggaa agccacactt cgccgacttc aacctcgtcc cagacggatg gggaggatac
    10801 gtctacccat ggatcgaggc catgtcctcc tcctacacct ggctcgagga gatccgtctc
    10861 aagcgtatgg tcgtcaccga cgactgcctc gagctcatcg ccaagtcctt caagaacttc
    10921 aaggtcctcg tcctctcctc ctgcgaggga ttctccaccg acggactcgc cgccatcgcc
    10981 gccacctgcc gtaacctcaa ggtaagttta aacatatata tactaactaa ccctgattat
    11041 ttaaattttc aggagctcga cctccgtgag tccgacgtcg acgaggtctc cggacactgg
    11101 ctctcccact tcccagacac ctacacctcc ctcgtctccc tcaacatctc ctgcctcgcc
    11161 tccgaggtct ccttctccgc cctcgagcgt ctcgtcaccc gttgcccaaa cctcaagtcc
    11221 ctcaagctca accgtgccgt cccactcgag aagctcgcca ccctcctcca acgtgcccca
    11281 caactcgagg agctcggaac cggaggatac accgccgagg tccgtccaga cgtctactcc
    11341 ggactctccg tcgccctctc cggatgcaag gtaagtttaa acagttcggt actaactaac
    11401 catacatatt taaattttca ggagctccgt tgcctctccg gattctggga cgccgtccca
    11461 gcctacctcc cagccgtcta ctccgtctgc tcccgtctca ccaccctcaa cctctcctac
    11521 gccaccgtcc aatcctacga cctcgtcaag ctcctctgcc aatgcccaaa gctccaacgt
    11581 ctctgggtcc tcgactacat cgaggacgcc ggactcgagg tcctcgcctc cacctgcaag
    11641 gacctccgtg agctccgtgt cttcccatcc gagccattcg tcatggagcc aaacgtcgcc
    11701 ctcaccgagc aaggactcgt ctccgtctcc atgggatgcc caaagctcga gtccgtcctc
    11761 tacttctgcc gtcaaatgac caacgccgcc ctcatcacca tcgcccgtaa ccgtccaaac
    11821 atgacccgtt tccgtctctg catcatcgag ccaaaggccc cagactacct caccctcgag
    11881 ccactcgaca tcggattcgg agccatcgtc gagcactgca aggacctccg tcgtctctcc
    11941 ctctccggac tcctcaccga caaggtcttc gagtacatcg gaacctacgc caagaagatg
    12001 gagatgctct ccgtcgcctt cgccggagac tccgacctcg gactccacca cgtcctctcc
    12061 ggatgcgact ccctccgtaa gctcgagatc cgtgactgcc cattcggaga caaggccctc
    12121 ctcgccaacg cctccaagct cgagaccatg cgttccctct ggatgtcctc ctgctccgtc
    12181 tccttcggag cctgcaagct cctcggacaa aagatgccaa agctcaacgt cgaggtcatc
    12241 gacgagcgtg gagccccaga ctcccgtcca gagtcctgcc cagtcgagcg tgtcttcatc
    12301 taccgtaccg tcgccggacc acgtttcgac atgccaggat tcgtctggaa catggaccaa
    12361 gactccacca tgcgtttctc ccgtcaaatc atcaccacca acggactcgg aggaggagga
    12421 tccccagtca agcaaaccct caacttcgac ctcctcaagc tcgccggaga cgtcgagtcc
    12481 aacccaggac caatggtttc ggaattgatt aaggaaaata tgcacatgaa gctctacatg
    12541 gagggaaccg tcgacaacca ccacttcaag tgcacctccg agggagaggg aaagccatac
    12601 gagggaaccc aaaccatgcg tatcaaggtc gtcgagggag gaccactccc attcgccttc
    12661 gacatcctcg ccacctcctt cctctacgga tccaaggtaa gtttaaacat atatatacta
    12721 actaaccctg attatttaaa ttttcagacc ttcatcaacc acacccaagg aatcccagac
    12781 ttcttcaagc aatccttccc agagggattc acctgggagc gtgtcaccac ctacgaggac
    12841 ggaggagtcc tcaccgccac ccaagacacc tccctccaag acggatgcct catctacaac
    12901 gtcaaggtaa gtttaaacag ttcggtacta actaaccata catatttaaa ttttcagatc
    12961 cgtggagtca acttcacctc caacggacca gtcatgcaaa agaagaccct cggatgggag
    13021 gccttcaccg agaccctcta cccagccgac ggaggactcg agggacgtaa cgacatggcc
    13081 ctcaagctcg tcggaggatc ccacctcatc gccaacgcca aggtaagttt aaacatgatt
    13141 ttactaacta actaatctga tttaaatttt cagaccacct accgttccaa gaagccagcc
    13201 aagaacctca agatgccagg agtctactac gtcgactacc gtctcgagcg tatcaaggag
    13261 gccaacaacg agacctacgt cgagcaacac gaggtcgccg tcgcccgtta ctgcgacctc
    13321 ccatccaagc tcggacacaa gctcaaccct aaagatccag ccaaacctcc ggccaaggca
    13381 caagttgtgg gatggccacc ggtgagatca taccggaaga acgtgatggt ttcctgccaa
    13441 aaatcaagcg gtggcccgga ggcggcggcg ttcgtgaaac cagccgccaa gcgtgtcaag
    13501 ctcgactaag ataaatgcaa aatcctttca agcattccct tcttctctat cactcttctt
    13561 tctttttgtc aaaaaattct ctcgctaatt tatttgcttt tttaatgtta ttattttatg
    13621 actttttata gtcactgaaa agtttgcatc tgagtgaagt gaatgctatc aaaatgtgat
    13681 tctgtctgat gtactttcac aatctctctt caattccatt ttgaagtgct ttaaacccga
    13741 aaggttgaga aaaatgcgag cgctcaaata tttgtattgt gttcgttgag tgacccaaca
    13801 aaaagaggaa actttattgt gccgccaaga aaaaagtctc acgtacggtg cgcgcgatgc
    13861 cctaggcatg gtcatagctg tttcctgcgc aagtcggcga tttctttgaa gttttgtcaa
    13921 aagaagagac aaggacttga tagatacgct catcagagtg agattcattg caaatagtgt
    13981 gtaaatcgtg gagagacgag agaaaaggtg tgagaagctt agaagataaa gaataggaag
    14041 aagaacatat ctcatttcct attttctcta atttttcgtt catttttcta gacattaccc
    14101 atttgatggt ttttcaatat ggttatcagg gtactcattt tggtagtttt caaagcaatt
    14161 tggagttttt ttttaaattg tcaactacaa aaacgcagct taaaggtcca aatatcatag
    14221 tataaaaatt ctttaaaaaa ttccaaattt tatatgattt tttgaaaatt ggaagaatgt
    14281 tagtttttgc catttttttg gaagtttttc tccggatttt atggtactta tatttcaaat
    14341 ataaattttt gtaggaattc gagtatgcga gagagctata gtttactaaa aaaaagtggc
    14401 aaaaactcag taattgccac ttttttcaaa aatgttttga aaaattttac tatgatattt
    14461 ggccattttg gcaccatatg tacgttactt tttacgaaac actctaattt ttgaaatttg
    14521 agtttcatgt ttaatctttt gtataaaatt tttatggtcc ttttgtgaca ggataaaaaa
    14581 ttgcaaaaaa atagaaaact ttgtttcgtg aaaacaccta gtttaaaaat tgtgggccac
    14641 ttagcgattc tagtcaaaaa tcagacaaat gtcagctgcc gtaacttttt ccggatcagg
    14701 gttatcaaat tttaatttta attgtaaatg tagaaaaatg atcggcaaca ttttgctaat
    14761 tgaaaatatt ttgaaaattg ctatagtaca tattaatcta gtattaatat agtattaatg
    14821 ttataatatc agttatgtgt ttgaataact caagcgtaag tttccgtaaa atatctttaa
    14881 attttggtaa ttttttacgg tttggtataa atttttgcat tttttgattt tctacacttt
    14941 ttttcgattt cgcattttcc gaatttctct gaaataaatc gaacaatcgg aaaattttcg
    15001 agggcaccct tagccatgca gctcaggaat ttgactttat gttcccattc aaattttttt
    15061 aaaattaaat aaaatgctaa ttgtaagata aattacaaaa attattaaat ctcatcatcc
    15121 ttatgatgat agtgcagtgc atgaatatat ttttccaaat aggatatgat tcgtcgagta
    15181 gcttcacgta atccgttttc ttcaatcacc atttcgaact gcaatttttt ttttggattt
    15241 ttgtaacaat attgtcataa attgtgattt tgatattttg cgtagagcat cagaaccttt
    15301 ctgctttgaa ttttaatggg attaaaaatt ataaaaaact tgcaccctaa ttaaagcgcc
    15361 aactttatta tacatagttg ataattcgat ggtatcagct cactcaaagg cggtaatacg
    15421 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    15481 gaccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    15541 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    15601 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    15661 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    15721 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    15781 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    15841 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    15901 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    15961 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    16021 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    16081 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    16141 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    16201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    16261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    16321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    16381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    16441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    16501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    16561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    16621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    16681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    16741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    16801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    16861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    16921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    16981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    17041 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    17101 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    17161 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    17221 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    17281 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    17341 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    17401 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    17461 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    17521 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    17581 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    17641 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggatccg
    17701 ccaagctatc aactttgtat agaaaagttg
//
