LOCUS       sequence-335480-       14922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Intestinal promoter ges-1P::TIR1::F2A::TagBFP2::AID* with homology 
            arms for insertion in ttTi5605 site on chromosome II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14922)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 14922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pTD80
FEATURES             Location/Qualifiers
     source          1..14922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1338..1371)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1371..1382)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2804..3127)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5045..5069
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5072..6097
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5072..6097
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6098..6122)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6475..6508)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     6877..6894
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6878..6894
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             10201..10266
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             11122..11253
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             11254..11280
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     terminator      11289..11612
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(12783..12802)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12902..12924
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12962..12980)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13048..13152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13153..13221,13222..14013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13371..13390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14184..14772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14673..14692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtcggatt atgggctctt cttttatcct ctactttctt tctagaaaat gcaaatgttg
       61 agtcagaaat tgctgaatgg agtacaactg caatgggttt agcttatgtt tctggaacaa
      121 ttactggtgg aattgtgatt gaaaagtaag gttatatttc atttcaataa atgttaccac
      181 agtataacaa aaacaagtta cattgttcag agtatgtctg aatgtattat tattattgta
      241 tttgtgcatc agtactgtag ttgtactgaa gaagggttta aaataatctc aacgttttta
      301 agacgagtta gtgagaatac ggtcgcagtt aggatgtttg gacatagttt tattagataa
      361 tcgtgaacta actcactata aaaacccgct aactcattga ataagatagt tttaaacgtt
      421 caaaatttga agacaaaatt gtatgaataa gtttacagag ttggtcgacg caagcttctc
      481 cttctcttca catttctcaa taatttagct ctaattgcat tcgtattctt cgcaaaaata
      541 cgtattctga ttgacccgat gaaatacgga tgcctagttg cattgatcat ctacggttat
      601 acttatgggt gaggtttttc aaatatattt tgatttaatc gaataatgaa atttcagaac
      661 tggagttggt ccaatttcgt ggtttatatc ctctgaactg gtacctcaaa aacatcgtag
      721 tattgctcaa tctgtggcat attcaatgaa tactgtaatg gtggttatca gtacatttac
      781 agtattacct ctatattccg ttattggtag ttatgctttc cttattctat attcaattcc
      841 ttcatttata agtatgctta tattatttcg ctacttaccg gaaaccaaag gacgagagat
      901 tcatgatatt gtgaatgaac ttaaaaaaaa atgaaaacct ttgaaaacgt tcatacggcc
      961 gaaattttac ttgatcgagc caattgaact tttgttaaga cttacagaaa agtgagagat
     1021 tttgttacaa aaaagtgaga aaaaagcgaa aatgaacagg aaaaagaaga aaaatacacc
     1081 cgaagtatca tataaaaatt aaaaagattg tttgacctgg cggaactttt ttgcaaaaac
     1141 tctgaatatc catggcacat gcttcgtgca aaacaggatc caaatgaata tcaacaagag
     1201 attcttgaag acgacgagcc acttgctcag agcattgctt atctcgaatg agacccttgt
     1261 taaaatcatt tttcaaacat tatatgacat tgtcgaaatg tcctcctgat tccatgatgg
     1321 tagcaaactc acttcgtata acttcgtata gtatacctta tacgaagtta tctgccctcg
     1381 attgtctatc ttccattctc accaagtttc aacaacttta ttcagtcatt cttctctggt
     1441 tcatttccca accaatcttc ccgaataaag cccatctgtc ctttcggcca cattcctcac
     1501 ctacatccgt actccttatc tcccgttctt atccagtctt ttatcgtctc tgcgtctcgc
     1561 acactgtctc gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact tgcggaaaga
     1621 tttcctaata aaaggagacg aatccggtag gaaatcgaca agtctctgct tctcgaagac
     1681 taggtgagtt acatttcttt ggccgaatta ttttgaaatc ctaaatttga taatttcaga
     1741 gatgtctgta aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca ctcttatggt
     1801 atgcttcatg accatgtcaa ccatctactc ggaggttgat ggattcagag agaagcttga
     1861 taccgagatg aatgtgttca gagtgagtta aattatattt tgaattttaa ataattttta
     1921 attttctagc aatctaccaa tggattgtgg aaggacatag ttgtcatcgg aagatctagc
     1981 aagcgtgtcc gttgtcaata tgaagagacc aacgctaccc caactccaca tgctgatgga
     2041 tccccatctg ctccaccagg tcaaccacca gcagttccac cagtcttcaa ccagccaaag
     2101 actccaaatg gagccaatgg aaatggacca acctgcaact gcaatgctga taacaagtgc
     2161 ccagctggac catccggacc aaagggagtt ccaggagttc caggactcga cggagttcca
     2221 ggacttgacg gtgttccagg agttggagct gatgatatcg ctccacaacg cgagtctgtc
     2281 ggatgcttca cttgcccaca aggaccagtt ggaccaccag gagctcttgg aagaccagga
     2341 ccacgtggac ttccaggacc aagaggacaa aatggaaacc caggaagaga tggacaacca
     2401 ggacatccag gagagcaagg atcatccggt caaatcggaa agatcggaga gccaggacca
     2461 ccaggagaga agggacgcga cgccgagcat ccaatcggaa gaccaggacc aaagggacca
     2521 agaggagatc aaggaccaac aggaccagct ggacagaacg gtcttcacgg accaccagga
     2581 gagccaggaa ccgttggacc agaaggacca tctggaaagc aaggacgtca aggaccagac
     2641 ggaacccagg gagagactgg accagacgga agaccaggaa aggatgccga gtactgccag
     2701 tgcccagaca agtctccacc atcagaggct gtcaacgcca accgtggata cagaaatatc
     2761 taaattgttg gtgttttcta ataaaatatt tgagattcag tgagactttt ttcttggcgg
     2821 cacaataaag tttcctcttt ttgttgggtc actcaacgaa cacaatacaa atatttgagc
     2881 gctcgcattt ttctcaacct ttcgggttta aagcacttca aaatggaatt gaagagagat
     2941 tgtgaaagta catcagacag aatcacattt tgatagcatt cacttcactc agatgcaaac
     3001 ttttcagtga ctataaaaag tcataaaata ataacattaa aaaagcaaat aaattagcga
     3061 gagaattttt tgacaaaaag aaagaagagt gatagagaag aagggaatgc ttgaaaggat
     3121 tttgcattta tcttaatcac cgtcttcgag aagacgaacc atggcgccag tttcagaatc
     3181 caagtttcta atatagttca tcacaatgtt gacgttggtc caacctccgg cttgcataat
     3241 ctctgggatg gaaactccag cgcgagccat gtcacgggca gctccgacac gggcggaatg
     3301 tccagaccaa gccaagtaac gttgtccgga atcatccttg gctccgtaga tgagacggtg
     3361 ggtagcttcg aagattcctg aaaatttatt ttcagcttat tcttagctaa tatgttgttt
     3421 aaacttacct tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg cagcaactcc
     3481 gttcttgcga acacgacaga acaggtagtt gtttggatca tcggcgactc cggaaaccga
     3541 aatccagcgc tcaacgagct tggtaactcc aagggagagc gctttttcga ctccggcagt
     3601 cgagaccagc gtttttgtac gtccgatgtg gatgagcatt cttcctccgt ctgtgcgact
     3661 aatatccttg acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt tgtaagcaat
     3721 tccgaggaag gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca taagagaacg
     3781 gacctgatcg aagtcggttc tgaaaatttc gtcaagtctt aggattgaat acattaatat
     3841 ttaaacttac ctttcgaagg cgagggcttg cttggctctc tctccagcat caacattttc
     3901 cttgcggata cgtctcatta cgagagatac ggcattagaa tctgatgggc gtggaagtcc
     3961 agatcggcga tggagcatat tgagctgtcc gagatgttgt tggatcgtct tcaccgccaa
     4021 tccacgagct tggaggtaga gcaagtagtc acgaacgtct tctggctcgg ctgggaacca
     4081 cttgcggttg ttaagcttgc accaggcagc ccaagaacgg cacactgaga gaagcatctt
     4141 ccaggtgtgc tcggagaagg cttgtctgtc acggaacata tccatgaggt tcttgcgaac
     4201 ttcatcactt gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag tgagaagatt
     4261 tgacatattt tcgaagtttt ttagatgcac tagaacaaag cgtgttggct tcctctgagc
     4321 ccgctttcct tatatacccg cattctgcag ccttacagaa tgttctagaa ggtcctagat
     4381 gcattcgttt gaaaatactc ccggtgggtg caaagagacg cagacggaaa atgtatctgg
     4441 gtctctttat tgtgtacact acttttccat gtaccgaatg tgagtcgccc tccttttgca
     4501 acaagcagct cgaatgttct agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa
     4561 ataccgaaca acatttgctc taattgtgaa attagaaatc ttcaaactat aatcatgtca
     4621 ctttaccact atttccgtcc agctcaacgt tccgtttttg gtgatcattt ttgctttcgt
     4681 cgtaaatcta cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc aaattcgttg
     4741 actgagtatt caacgtttat acgttgtcgg caacgagaaa taggaaaatg catcgggaaa
     4801 tgttcttttt tcgatttttt ccaaggtttt gacaaatttt accacgaatt ttgctatgtt
     4861 ttcaattaaa aaatatgtta ttcaactgtt tctatgagga aaataaggct ttgcatgtaa
     4921 ttttcttatt cagcataatt tttaattaat ttgaattttc tgtcctaacg tttattttgt
     4981 tttcttggtt atgactgatc tgaaattaat ttttgaattt taaggtaata tgtcaggcgg
     5041 tgccgcaagt ttgtacaaaa aagcaggctc catgaaaaag cctgaactca ccgcgacgtc
     5101 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     5161 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     5221 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     5281 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     5341 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     5401 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     5461 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     5521 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     5581 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     5641 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     5701 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     5761 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     5821 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     5881 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     5941 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     6001 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     6061 ccgacgcccc agcactcgtc cgagggcaaa ggaatagacc cagctttctt gtacaaagtg
     6121 ggtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt
     6181 tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc
     6241 tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa
     6301 aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca caatgttctg
     6361 tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc
     6421 gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt tttaataact
     6481 tcgtatagta taccttatac gaagttatac tagtatatat ttttatgttt tcttttaaac
     6541 cttcctccat atttctgaat tgaatgttgt tgcatattgt gtgtccttat ttcatttcca
     6601 tcatcctttt gtgtgtttcc cccttccacc aattcccccc acgtcctaat gaaatgaaag
     6661 cgagaagata aataaacttg cacattgtgt tcagatgaga ggagcggaat acgaattggg
     6721 agacggaaag agaaaaaaaa atgtttagat ttggattgaa ttaatttttt agaaagcaag
     6781 aaaaaaaaca caaagagaat aaataaataa taggaaaaaa aacaaatatt cagtatttaa
     6841 aatttgaaaa ttcaacgacg ttggcgtcga tcatcctgta aaacgacggc cagtgcatgc
     6901 ctcgacttta atattctttt ctggtttcac cagaaaaatg ctggactgaa aaattgaatt
     6961 tcccgcgatt tgggaaaaac cgcagtttaa gcttttggca tgaatacagt gaacacagga
     7021 acaaaaaatt gacttgttat tttgagttta tattaaatgt taattttgca tctcatattt
     7081 tcacaaaact tgtttgcttg aaaaatgtgc aaataacaag tgaacataca tcaaggttta
     7141 tgcagatcag cactgatgca actgatagca aaactgataa gggtcaaaat ttcagtggcc
     7201 agcacaaaca catacacatt cgtagttcgt tttgtgtttc gtgtatttag catttatttt
     7261 tcaattccgg caatttgccg atttgccgga aattttgatt tttggcaaat tgccagtttg
     7321 ccgtttgccg aacatcaatt tgccgaaatt ttttagaggg atctttataa gacggaaaca
     7381 cttaaaattg tgcttttttg gaattttttt caatgtttca atagaattag cttacattta
     7441 aaattagatg taggaacgtt tataggacgc ttacaatttt gccgattaaa atcgaagttc
     7501 tgaacttttc aaaaaatgtg caaaattttt tttgacaaat ttgacaaatc ggccggtttg
     7561 tcgattgtcc ggaatttttc agtttcggcg atttaccggt ttgccggaaa acatcgtttg
     7621 ccacccacac acacataaag agacgaaaca acacaccatc ttcaaaaaat ttgtgaatct
     7681 tcatttccac gtggtttgca gggtttccca tccaccccac tctatctctc caagttgaat
     7741 tttggcaaaa ttgaccacac accgatcaaa aactattgtg tgtgtgtgta tttgtgtctt
     7801 caataattcc tgtctgtgtc tcgcactatc tattgactac aagaacgtga tcgcagctct
     7861 acgaaacctt tactttattt tagaaatatt ttttcatgct attagcaaaa aaaaagtttt
     7921 tttttgtcta agtacactta aaattttcca aaatttttaa gatttttcaa aatgtgcgat
     7981 caaagatctg acaaaatgcc aaaattttga agaggaaatt tagtcggctt ccaaaattat
     8041 aagtggcaga aattgagcaa ttgtcacttt ttgacagtaa atagaaaatt ttttgaaaaa
     8101 ttttgaaacg ctttattttg ttacttggtc attttggcac cataggagta gtttttaaca
     8161 ttttccccac tggcgctact tcattttaaa cttttccaaa gattttaatt tttaatttta
     8221 aaaaatttca tataattcgt ctgcgtctta cagtttcagg cttcaaaaat ctattacata
     8281 tcttatcttt gaattcagat gcaaaagaga atcgccttgt cgtttccaga agaagtactc
     8341 gagcacgtct tctccttcat ccaactcgac aaggaccgta actccgtctc cctcgtctgc
     8401 aagtcctggt acgagatcga gcgttggtgc cgtcgtaagg tcttcatcgg aaactgctac
     8461 gccgtctccc cagccaccgt catccgtcgt ttcccaaagg tccgttccgt cgagctcaag
     8521 ggaaagccac acttcgccga cttcaacctc gtcccagacg gatggggagg atacgtctac
     8581 ccatggatcg aggccatgtc ctcctcctac acctggctcg aggagatccg tctcaagcgt
     8641 atggtcgtca ccgacgactg cctcgagctc atcgccaagt ccttcaagaa cttcaaggtc
     8701 ctcgtcctct cctcctgcga gggattctcc accgacggac tcgccgccat cgccgccacc
     8761 tgccgtaacc tcaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
     8821 tttcaggagc tcgacctccg tgagtccgac gtcgacgagg tctccggaca ctggctctcc
     8881 cacttcccag acacctacac ctccctcgtc tccctcaaca tctcctgcct cgcctccgag
     8941 gtctccttct ccgccctcga gcgtctcgtc acccgttgcc caaacctcaa gtccctcaag
     9001 ctcaaccgtg ccgtcccact cgagaagctc gccaccctcc tccaacgtgc cccacaactc
     9061 gaggagctcg gaaccggagg atacaccgcc gaggtccgtc cagacgtcta ctccggactc
     9121 tccgtcgccc tctccggatg caaggtaagt ttaaacagtt cggtactaac taaccataca
     9181 tatttaaatt ttcaggagct ccgttgcctc tccggattct gggacgccgt cccagcctac
     9241 ctcccagccg tctactccgt ctgctcccgt ctcaccaccc tcaacctctc ctacgccacc
     9301 gtccaatcct acgacctcgt caagctcctc tgccaatgcc caaagctcca acgtctctgg
     9361 gtcctcgact acatcgagga cgccggactc gaggtcctcg cctccacctg caaggacctc
     9421 cgtgagctcc gtgtcttccc atccgagcca ttcgtcatgg agccaaacgt cgccctcacc
     9481 gagcaaggac tcgtctccgt ctccatggga tgcccaaagc tcgagtccgt cctctacttc
     9541 tgccgtcaaa tgaccaacgc cgccctcatc accatcgccc gtaaccgtcc aaacatgacc
     9601 cgtttccgtc tctgcatcat cgagccaaag gccccagact acctcaccct cgagccactc
     9661 gacatcggat tcggagccat cgtcgagcac tgcaaggacc tccgtcgtct ctccctctcc
     9721 ggactcctca ccgacaaggt cttcgagtac atcggaacct acgccaagaa gatggagatg
     9781 ctctccgtcg ccttcgccgg agactccgac ctcggactcc accacgtcct ctccggatgc
     9841 gactccctcc gtaagctcga gatccgtgac tgcccattcg gagacaaggc cctcctcgcc
     9901 aacgcctcca agctcgagac catgcgttcc ctctggatgt cctcctgctc cgtctccttc
     9961 ggagcctgca agctcctcgg acaaaagatg ccaaagctca acgtcgaggt catcgacgag
    10021 cgtggagccc cagactcccg tccagagtcc tgcccagtcg agcgtgtctt catctaccgt
    10081 accgtcgccg gaccacgttt cgacatgcca ggattcgtct ggaacatgga ccaagactcc
    10141 accatgcgtt tctcccgtca aatcatcacc accaacggac tcggaggagg aggatcccca
    10201 gtcaagcaaa ccctcaactt cgacctcctc aagctcgccg gagacgtcga gtccaaccca
    10261 ggaccaatgg tttcggaatt gattaaggaa aatatgcaca tgaagctcta catggaggga
    10321 accgtcgaca accaccactt caagtgcacc tccgagggag agggaaagcc atacgaggga
    10381 acccaaacca tgcgtatcaa ggtcgtcgag ggaggaccac tcccattcgc cttcgacatc
    10441 ctcgccacct ccttcctcta cggatccaag gtaagtttaa acatatatat actaactaac
    10501 cctgattatt taaattttca gaccttcatc aaccacaccc aaggaatccc agacttcttc
    10561 aagcaatcct tcccagaggg attcacctgg gagcgtgtca ccacctacga ggacggagga
    10621 gtcctcaccg ccacccaaga cacctccctc caagacggat gcctcatcta caacgtcaag
    10681 gtaagtttaa acagttcggt actaactaac catacatatt taaattttca gatccgtgga
    10741 gtcaacttca cctccaacgg accagtcatg caaaagaaga ccctcggatg ggaggccttc
    10801 accgagaccc tctacccagc cgacggagga ctcgagggac gtaacgacat ggccctcaag
    10861 ctcgtcggag gatcccacct catcgccaac gccaaggtaa gtttaaacat gattttacta
    10921 actaactaat ctgatttaaa ttttcagacc acctaccgtt ccaagaagcc agccaagaac
    10981 ctcaagatgc caggagtcta ctacgtcgac taccgtctcg agcgtatcaa ggaggccaac
    11041 aacgagacct acgtcgagca acacgaggtc gccgtcgccc gttactgcga cctcccatcc
    11101 aagctcggac acaagctcaa ccctaaagat ccagccaaac ctccggccaa ggcacaagtt
    11161 gtgggatggc caccggtgag atcataccgg aagaacgtga tggtttcctg ccaaaaatca
    11221 agcggtggcc cggaggcggc ggcgttcgtg aaaccagccg ccaagcgtgt caagctcgac
    11281 taagataaat gcaaaatcct ttcaagcatt cccttcttct ctatcactct tctttctttt
    11341 tgtcaaaaaa ttctctcgct aatttatttg cttttttaat gttattattt tatgactttt
    11401 tatagtcact gaaaagtttg catctgagtg aagtgaatgc tatcaaaatg tgattctgtc
    11461 tgatgtactt tcacaatctc tcttcaattc cattttgaag tgctttaaac ccgaaaggtt
    11521 gagaaaaatg cgagcgctca aatatttgta ttgtgttcgt tgagtgaccc aacaaaaaga
    11581 ggaaacttta ttgtgccgcc aagaaaaaag tctcacgtac ggtgcgcgcg atgccgtatc
    11641 acaactgaga aaaaagacaa aacttattga acagtgttcc tttcaaaaaa ttattttgta
    11701 aaaaatcgag tttttctcca aaataacgca cgcaatcaaa aacaagaaaa ctaacgtgtt
    11761 ctcctgggcg tggaactcct tatcaagatt accattattg tctgtcagaa ttgatatttc
    11821 aattatttca tatctagaca aacccgggtg tcttgcattt attagtactg tgagtttaca
    11881 tacaaacgag gcttactcag ataacataga gtcacctcta attagcaaaa acccatttct
    11941 aattagctat ttcgaccagt tctaaagtca ccgtaattag aaatggtttt tgtagctttt
    12001 ctaattagaa atgaccccga gataagtgaa atatggcatt ttctgggttt tctagtaaga
    12061 aatgatcccg cacgtagtga ttccgcgtta caagccaaat tcaaattaaa aaagtatatt
    12121 acacaattca agttacatta ttttcaggaa acaatggcga agagcaaagc agagattcaa
    12181 aaggaggaaa gagacaacag atatgaacga agacttcttg aaaagagaaa gagacatgag
    12241 gtgagtaacc atgataataa ataatcaagt aagggctata gatttataaa ttctggagtt
    12301 aacagtttcg aaaaaaagta gttttcatat gtgcgaagga atatcaaaaa taggggaata
    12361 gcctaaacga gtgagaaaat taaatgtttg ttattttttc aaaaatgtct gcagggccgt
    12421 tatcctttca aatgcactct atcaaacata gatcatatcc atcatatctt atccagaatt
    12481 tatatcatat ccaagattga gagcgtttca aggaaaatct caaacaacca tacaattttg
    12541 tagatcgaca acaaggcctc cacttcttcc acgtgacgtc gtgactggga aaaccctggc
    12601 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    12661 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
    12721 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
    12781 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
    12841 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
    12901 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
    12961 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
    13021 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    13081 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    13141 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    13201 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    13261 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    13321 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    13381 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    13441 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    13501 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    13561 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    13621 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    13681 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    13741 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    13801 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    13861 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    13921 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    13981 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    14041 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    14101 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    14161 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    14221 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    14281 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
    14341 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    14401 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    14461 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    14521 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    14581 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    14641 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    14701 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    14761 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggtctt ttgctggcct
    14821 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    14881 tttgagtgag ctgataccag cggataacaa tttcacaggg cc
//
