LOCUS       sequence-335481-       14671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology 
            arms for insertion in ttTi5605 site on chromosome II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14671)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 14671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pTD81
FEATURES             Location/Qualifiers
     source          1..14671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1338..1371)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1371..1382)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2804..3127)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5045..5069
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5072..6097
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5072..6097
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6098..6122)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6475..6508)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     6877..6894
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6878..6894
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9950..10015
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             10871..11002
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             11003..11029
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     terminator      11038..11361
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(12532..12551)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12651..12673
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12711..12729)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12797..12901
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12902..12970,12971..13762)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13120..13139)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13933..14521
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14422..14441
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtcggatt atgggctctt cttttatcct ctactttctt tctagaaaat gcaaatgttg
       61 agtcagaaat tgctgaatgg agtacaactg caatgggttt agcttatgtt tctggaacaa
      121 ttactggtgg aattgtgatt gaaaagtaag gttatatttc atttcaataa atgttaccac
      181 agtataacaa aaacaagtta cattgttcag agtatgtctg aatgtattat tattattgta
      241 tttgtgcatc agtactgtag ttgtactgaa gaagggttta aaataatctc aacgttttta
      301 agacgagtta gtgagaatac ggtcgcagtt aggatgtttg gacatagttt tattagataa
      361 tcgtgaacta actcactata aaaacccgct aactcattga ataagatagt tttaaacgtt
      421 caaaatttga agacaaaatt gtatgaataa gtttacagag ttggtcgacg caagcttctc
      481 cttctcttca catttctcaa taatttagct ctaattgcat tcgtattctt cgcaaaaata
      541 cgtattctga ttgacccgat gaaatacgga tgcctagttg cattgatcat ctacggttat
      601 acttatgggt gaggtttttc aaatatattt tgatttaatc gaataatgaa atttcagaac
      661 tggagttggt ccaatttcgt ggtttatatc ctctgaactg gtacctcaaa aacatcgtag
      721 tattgctcaa tctgtggcat attcaatgaa tactgtaatg gtggttatca gtacatttac
      781 agtattacct ctatattccg ttattggtag ttatgctttc cttattctat attcaattcc
      841 ttcatttata agtatgctta tattatttcg ctacttaccg gaaaccaaag gacgagagat
      901 tcatgatatt gtgaatgaac ttaaaaaaaa atgaaaacct ttgaaaacgt tcatacggcc
      961 gaaattttac ttgatcgagc caattgaact tttgttaaga cttacagaaa agtgagagat
     1021 tttgttacaa aaaagtgaga aaaaagcgaa aatgaacagg aaaaagaaga aaaatacacc
     1081 cgaagtatca tataaaaatt aaaaagattg tttgacctgg cggaactttt ttgcaaaaac
     1141 tctgaatatc catggcacat gcttcgtgca aaacaggatc caaatgaata tcaacaagag
     1201 attcttgaag acgacgagcc acttgctcag agcattgctt atctcgaatg agacccttgt
     1261 taaaatcatt tttcaaacat tatatgacat tgtcgaaatg tcctcctgat tccatgatgg
     1321 tagcaaactc acttcgtata acttcgtata gtatacctta tacgaagtta tctgccctcg
     1381 attgtctatc ttccattctc accaagtttc aacaacttta ttcagtcatt cttctctggt
     1441 tcatttccca accaatcttc ccgaataaag cccatctgtc ctttcggcca cattcctcac
     1501 ctacatccgt actccttatc tcccgttctt atccagtctt ttatcgtctc tgcgtctcgc
     1561 acactgtctc gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact tgcggaaaga
     1621 tttcctaata aaaggagacg aatccggtag gaaatcgaca agtctctgct tctcgaagac
     1681 taggtgagtt acatttcttt ggccgaatta ttttgaaatc ctaaatttga taatttcaga
     1741 gatgtctgta aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca ctcttatggt
     1801 atgcttcatg accatgtcaa ccatctactc ggaggttgat ggattcagag agaagcttga
     1861 taccgagatg aatgtgttca gagtgagtta aattatattt tgaattttaa ataattttta
     1921 attttctagc aatctaccaa tggattgtgg aaggacatag ttgtcatcgg aagatctagc
     1981 aagcgtgtcc gttgtcaata tgaagagacc aacgctaccc caactccaca tgctgatgga
     2041 tccccatctg ctccaccagg tcaaccacca gcagttccac cagtcttcaa ccagccaaag
     2101 actccaaatg gagccaatgg aaatggacca acctgcaact gcaatgctga taacaagtgc
     2161 ccagctggac catccggacc aaagggagtt ccaggagttc caggactcga cggagttcca
     2221 ggacttgacg gtgttccagg agttggagct gatgatatcg ctccacaacg cgagtctgtc
     2281 ggatgcttca cttgcccaca aggaccagtt ggaccaccag gagctcttgg aagaccagga
     2341 ccacgtggac ttccaggacc aagaggacaa aatggaaacc caggaagaga tggacaacca
     2401 ggacatccag gagagcaagg atcatccggt caaatcggaa agatcggaga gccaggacca
     2461 ccaggagaga agggacgcga cgccgagcat ccaatcggaa gaccaggacc aaagggacca
     2521 agaggagatc aaggaccaac aggaccagct ggacagaacg gtcttcacgg accaccagga
     2581 gagccaggaa ccgttggacc agaaggacca tctggaaagc aaggacgtca aggaccagac
     2641 ggaacccagg gagagactgg accagacgga agaccaggaa aggatgccga gtactgccag
     2701 tgcccagaca agtctccacc atcagaggct gtcaacgcca accgtggata cagaaatatc
     2761 taaattgttg gtgttttcta ataaaatatt tgagattcag tgagactttt ttcttggcgg
     2821 cacaataaag tttcctcttt ttgttgggtc actcaacgaa cacaatacaa atatttgagc
     2881 gctcgcattt ttctcaacct ttcgggttta aagcacttca aaatggaatt gaagagagat
     2941 tgtgaaagta catcagacag aatcacattt tgatagcatt cacttcactc agatgcaaac
     3001 ttttcagtga ctataaaaag tcataaaata ataacattaa aaaagcaaat aaattagcga
     3061 gagaattttt tgacaaaaag aaagaagagt gatagagaag aagggaatgc ttgaaaggat
     3121 tttgcattta tcttaatcac cgtcttcgag aagacgaacc atggcgccag tttcagaatc
     3181 caagtttcta atatagttca tcacaatgtt gacgttggtc caacctccgg cttgcataat
     3241 ctctgggatg gaaactccag cgcgagccat gtcacgggca gctccgacac gggcggaatg
     3301 tccagaccaa gccaagtaac gttgtccgga atcatccttg gctccgtaga tgagacggtg
     3361 ggtagcttcg aagattcctg aaaatttatt ttcagcttat tcttagctaa tatgttgttt
     3421 aaacttacct tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg cagcaactcc
     3481 gttcttgcga acacgacaga acaggtagtt gtttggatca tcggcgactc cggaaaccga
     3541 aatccagcgc tcaacgagct tggtaactcc aagggagagc gctttttcga ctccggcagt
     3601 cgagaccagc gtttttgtac gtccgatgtg gatgagcatt cttcctccgt ctgtgcgact
     3661 aatatccttg acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt tgtaagcaat
     3721 tccgaggaag gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca taagagaacg
     3781 gacctgatcg aagtcggttc tgaaaatttc gtcaagtctt aggattgaat acattaatat
     3841 ttaaacttac ctttcgaagg cgagggcttg cttggctctc tctccagcat caacattttc
     3901 cttgcggata cgtctcatta cgagagatac ggcattagaa tctgatgggc gtggaagtcc
     3961 agatcggcga tggagcatat tgagctgtcc gagatgttgt tggatcgtct tcaccgccaa
     4021 tccacgagct tggaggtaga gcaagtagtc acgaacgtct tctggctcgg ctgggaacca
     4081 cttgcggttg ttaagcttgc accaggcagc ccaagaacgg cacactgaga gaagcatctt
     4141 ccaggtgtgc tcggagaagg cttgtctgtc acggaacata tccatgaggt tcttgcgaac
     4201 ttcatcactt gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag tgagaagatt
     4261 tgacatattt tcgaagtttt ttagatgcac tagaacaaag cgtgttggct tcctctgagc
     4321 ccgctttcct tatatacccg cattctgcag ccttacagaa tgttctagaa ggtcctagat
     4381 gcattcgttt gaaaatactc ccggtgggtg caaagagacg cagacggaaa atgtatctgg
     4441 gtctctttat tgtgtacact acttttccat gtaccgaatg tgagtcgccc tccttttgca
     4501 acaagcagct cgaatgttct agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa
     4561 ataccgaaca acatttgctc taattgtgaa attagaaatc ttcaaactat aatcatgtca
     4621 ctttaccact atttccgtcc agctcaacgt tccgtttttg gtgatcattt ttgctttcgt
     4681 cgtaaatcta cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc aaattcgttg
     4741 actgagtatt caacgtttat acgttgtcgg caacgagaaa taggaaaatg catcgggaaa
     4801 tgttcttttt tcgatttttt ccaaggtttt gacaaatttt accacgaatt ttgctatgtt
     4861 ttcaattaaa aaatatgtta ttcaactgtt tctatgagga aaataaggct ttgcatgtaa
     4921 ttttcttatt cagcataatt tttaattaat ttgaattttc tgtcctaacg tttattttgt
     4981 tttcttggtt atgactgatc tgaaattaat ttttgaattt taaggtaata tgtcaggcgg
     5041 tgccgcaagt ttgtacaaaa aagcaggctc catgaaaaag cctgaactca ccgcgacgtc
     5101 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     5161 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     5221 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     5281 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     5341 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     5401 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     5461 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     5521 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     5581 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     5641 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     5701 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     5761 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     5821 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     5881 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     5941 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     6001 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     6061 ccgacgcccc agcactcgtc cgagggcaaa ggaatagacc cagctttctt gtacaaagtg
     6121 ggtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt
     6181 tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc
     6241 tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa
     6301 aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca caatgttctg
     6361 tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc
     6421 gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt tttaataact
     6481 tcgtatagta taccttatac gaagttatac tagtatatat ttttatgttt tcttttaaac
     6541 cttcctccat atttctgaat tgaatgttgt tgcatattgt gtgtccttat ttcatttcca
     6601 tcatcctttt gtgtgtttcc cccttccacc aattcccccc acgtcctaat gaaatgaaag
     6661 cgagaagata aataaacttg cacattgtgt tcagatgaga ggagcggaat acgaattggg
     6721 agacggaaag agaaaaaaaa atgtttagat ttggattgaa ttaatttttt agaaagcaag
     6781 aaaaaaaaca caaagagaat aaataaataa taggaaaaaa aacaaatatt cagtatttaa
     6841 aatttgaaaa ttcaacgacg ttggcgtcga tcatcctgta aaacgacggc cagtgcatgc
     6901 gatcttcatc ccttcaacat ttggcagaaa agtgcaattc gtcttttaca ggaattcgat
     6961 ttccgatctc ttgcacaact cgaggcgtca ctccagatgg attaggaact gtttcccttt
     7021 gtagtacatg ttgggtgtgg cgacgtcttc cgagtaccta ctatcctgca tatcttaacg
     7081 aagtcgtgtg cgattatgcg gacacatcgt gtctgagtgg tagtgaaaag acagagaaag
     7141 tgggggaaaa agaaaatacc attttttcag gatacgcgtc atgccaaact ggaacccaac
     7201 agttgaatgt gctacgaaat gacagcggga aattgattcc aatatcggta tcagctggaa
     7261 tcaattgtga atgtagatta gcagttggat caacacttga atcactcgta cttggacaag
     7321 gaatttccaa agcaatgccg ccaattgaca caactagtac aaaaccacca aatttggcaa
     7381 caagcacaac aagccattcc taatttattt gggtatttta ctcttgataa ttgtattaat
     7441 gatcatttaa tgttgttgaa tatcacacat ttaacatatt caatcgaaga aatataacga
     7501 cgttccacaa catccagtat acgtcttcct ctgtattgag ttttattctt tgacattgtg
     7561 tcatgtttca gaacatagaa atataccaaa gtgttgagaa gcattgtata attcaattga
     7621 atctttttca gtttacctct tctccttttt caccgtttcc gtcatcaaaa cgccgttatt
     7681 caaggtgaga ttatggcgat aaatgaatgc cattcacaaa attgcaagac actattaaag
     7741 aagaagaggt ttagagattt gtaccgatca tagcttcttc tgagaaagcg accttggcgg
     7801 ccgtacttga agagaagcaa tttttatcgc cgtagaagat gttgcctgcc acccaccctg
     7861 tacgttgttt ttttgcttat caacatctga attttgttgt cttttgcacc gcntactttt
     7921 actatggaat ttgaggaaat ataatatgaa ttgaagagaa tacgctacct gtcaaagaaa
     7981 cgtcacggtt agggtagtat aaaagggaag ggtaacactg aataaggtac ctggcttact
     8041 ttcagtcatg caaaagagaa tcgccttgtc gtttccagaa gaagtactcg agcacgtctt
     8101 ctccttcatc caactcgaca aggaccgtaa ctccgtctcc ctcgtctgca agtcctggta
     8161 cgagatcgag cgttggtgcc gtcgtaaggt cttcatcgga aactgctacg ccgtctcccc
     8221 agccaccgtc atccgtcgtt tcccaaaggt ccgttccgtc gagctcaagg gaaagccaca
     8281 cttcgccgac ttcaacctcg tcccagacgg atggggagga tacgtctacc catggatcga
     8341 ggccatgtcc tcctcctaca cctggctcga ggagatccgt ctcaagcgta tggtcgtcac
     8401 cgacgactgc ctcgagctca tcgccaagtc cttcaagaac ttcaaggtcc tcgtcctctc
     8461 ctcctgcgag ggattctcca ccgacggact cgccgccatc gccgccacct gccgtaacct
     8521 caaggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt ttcaggagct
     8581 cgacctccgt gagtccgacg tcgacgaggt ctccggacac tggctctccc acttcccaga
     8641 cacctacacc tccctcgtct ccctcaacat ctcctgcctc gcctccgagg tctccttctc
     8701 cgccctcgag cgtctcgtca cccgttgccc aaacctcaag tccctcaagc tcaaccgtgc
     8761 cgtcccactc gagaagctcg ccaccctcct ccaacgtgcc ccacaactcg aggagctcgg
     8821 aaccggagga tacaccgccg aggtccgtcc agacgtctac tccggactct ccgtcgccct
     8881 ctccggatgc aaggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
     8941 tcaggagctc cgttgcctct ccggattctg ggacgccgtc ccagcctacc tcccagccgt
     9001 ctactccgtc tgctcccgtc tcaccaccct caacctctcc tacgccaccg tccaatccta
     9061 cgacctcgtc aagctcctct gccaatgccc aaagctccaa cgtctctggg tcctcgacta
     9121 catcgaggac gccggactcg aggtcctcgc ctccacctgc aaggacctcc gtgagctccg
     9181 tgtcttccca tccgagccat tcgtcatgga gccaaacgtc gccctcaccg agcaaggact
     9241 cgtctccgtc tccatgggat gcccaaagct cgagtccgtc ctctacttct gccgtcaaat
     9301 gaccaacgcc gccctcatca ccatcgcccg taaccgtcca aacatgaccc gtttccgtct
     9361 ctgcatcatc gagccaaagg ccccagacta cctcaccctc gagccactcg acatcggatt
     9421 cggagccatc gtcgagcact gcaaggacct ccgtcgtctc tccctctccg gactcctcac
     9481 cgacaaggtc ttcgagtaca tcggaaccta cgccaagaag atggagatgc tctccgtcgc
     9541 cttcgccgga gactccgacc tcggactcca ccacgtcctc tccggatgcg actccctccg
     9601 taagctcgag atccgtgact gcccattcgg agacaaggcc ctcctcgcca acgcctccaa
     9661 gctcgagacc atgcgttccc tctggatgtc ctcctgctcc gtctccttcg gagcctgcaa
     9721 gctcctcgga caaaagatgc caaagctcaa cgtcgaggtc atcgacgagc gtggagcccc
     9781 agactcccgt ccagagtcct gcccagtcga gcgtgtcttc atctaccgta ccgtcgccgg
     9841 accacgtttc gacatgccag gattcgtctg gaacatggac caagactcca ccatgcgttt
     9901 ctcccgtcaa atcatcacca ccaacggact cggaggagga ggatccccag tcaagcaaac
     9961 cctcaacttc gacctcctca agctcgccgg agacgtcgag tccaacccag gaccaatggt
    10021 ttcggaattg attaaggaaa atatgcacat gaagctctac atggagggaa ccgtcgacaa
    10081 ccaccacttc aagtgcacct ccgagggaga gggaaagcca tacgagggaa cccaaaccat
    10141 gcgtatcaag gtcgtcgagg gaggaccact cccattcgcc ttcgacatcc tcgccacctc
    10201 cttcctctac ggatccaagg taagtttaaa catatatata ctaactaacc ctgattattt
    10261 aaattttcag accttcatca accacaccca aggaatccca gacttcttca agcaatcctt
    10321 cccagaggga ttcacctggg agcgtgtcac cacctacgag gacggaggag tcctcaccgc
    10381 cacccaagac acctccctcc aagacggatg cctcatctac aacgtcaagg taagtttaaa
    10441 cagttcggta ctaactaacc atacatattt aaattttcag atccgtggag tcaacttcac
    10501 ctccaacgga ccagtcatgc aaaagaagac cctcggatgg gaggccttca ccgagaccct
    10561 ctacccagcc gacggaggac tcgagggacg taacgacatg gccctcaagc tcgtcggagg
    10621 atcccacctc atcgccaacg ccaaggtaag tttaaacatg attttactaa ctaactaatc
    10681 tgatttaaat tttcagacca cctaccgttc caagaagcca gccaagaacc tcaagatgcc
    10741 aggagtctac tacgtcgact accgtctcga gcgtatcaag gaggccaaca acgagaccta
    10801 cgtcgagcaa cacgaggtcg ccgtcgcccg ttactgcgac ctcccatcca agctcggaca
    10861 caagctcaac cctaaagatc cagccaaacc tccggccaag gcacaagttg tgggatggcc
    10921 accggtgaga tcataccgga agaacgtgat ggtttcctgc caaaaatcaa gcggtggccc
    10981 ggaggcggcg gcgttcgtga aaccagccgc caagcgtgtc aagctcgact aagataaatg
    11041 caaaatcctt tcaagcattc ccttcttctc tatcactctt ctttcttttt gtcaaaaaat
    11101 tctctcgcta atttatttgc ttttttaatg ttattatttt atgacttttt atagtcactg
    11161 aaaagtttgc atctgagtga agtgaatgct atcaaaatgt gattctgtct gatgtacttt
    11221 cacaatctct cttcaattcc attttgaagt gctttaaacc cgaaaggttg agaaaaatgc
    11281 gagcgctcaa atatttgtat tgtgttcgtt gagtgaccca acaaaaagag gaaactttat
    11341 tgtgccgcca agaaaaaagt ctcacgtacg gtgcgcgcga tgccgtatca caactgagaa
    11401 aaaagacaaa acttattgaa cagtgttcct ttcaaaaaat tattttgtaa aaaatcgagt
    11461 ttttctccaa aataacgcac gcaatcaaaa acaagaaaac taacgtgttc tcctgggcgt
    11521 ggaactcctt atcaagatta ccattattgt ctgtcagaat tgatatttca attatttcat
    11581 atctagacaa acccgggtgt cttgcattta ttagtactgt gagtttacat acaaacgagg
    11641 cttactcaga taacatagag tcacctctaa ttagcaaaaa cccatttcta attagctatt
    11701 tcgaccagtt ctaaagtcac cgtaattaga aatggttttt gtagcttttc taattagaaa
    11761 tgaccccgag ataagtgaaa tatggcattt tctgggtttt ctagtaagaa atgatcccgc
    11821 acgtagtgat tccgcgttac aagccaaatt caaattaaaa aagtatatta cacaattcaa
    11881 gttacattat tttcaggaaa caatggcgaa gagcaaagca gagattcaaa aggaggaaag
    11941 agacaacaga tatgaacgaa gacttcttga aaagagaaag agacatgagg tgagtaacca
    12001 tgataataaa taatcaagta agggctatag atttataaat tctggagtta acagtttcga
    12061 aaaaaagtag ttttcatatg tgcgaaggaa tatcaaaaat aggggaatag cctaaacgag
    12121 tgagaaaatt aaatgtttgt tattttttca aaaatgtctg cagggccgtt atcctttcaa
    12181 atgcactcta tcaaacatag atcatatcca tcatatctta tccagaattt atatcatatc
    12241 caagattgag agcgtttcaa ggaaaatctc aaacaaccat acaattttgt agatcgacaa
    12301 caaggcctcc acttcttcca cgtgacgtcg tgactgggaa aaccctggcg ttacccaact
    12361 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
    12421 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
    12481 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
    12541 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
    12601 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
    12661 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
    12721 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
    12781 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    12841 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    12901 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    12961 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    13021 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    13081 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    13141 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    13201 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    13261 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    13321 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    13381 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    13441 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    13501 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    13561 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    13621 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    13681 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    13741 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    13801 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    13861 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    13921 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    13981 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    14041 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
    14101 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    14161 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    14221 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    14281 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    14341 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    14401 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    14461 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    14521 aaacgccagc aacgcggcct ttttacggtt cctggtcttt tgctggcctt ttgctcacat
    14581 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    14641 tgataccagc ggataacaat ttcacagggc c
//
