LOCUS       sequence-335483-       10453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 B.1.1.7 Spike Protein lacking 18 C-terminal amino
            acids for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Garcia-Beltran WF, Lam EC, St Denis K, Nitido AD, Garcia ZH, Hauser 
            BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, 
            Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB
  TITLE     Multiple SARS-CoV-2 variants escape neutralization by 
            vaccine-induced humoral immunity.
  JOURNAL   Cell. 2021 Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013.
  PUBMED    33743213
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 B.1.1.7
FEATURES             Location/Qualifiers
     source          1..10453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    37..108
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(113..159)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    177..225
                     /note="synthetic polyadenylation signal"
     enhancer        306..609
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        611..813
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     764..784
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          947..1422
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     misc_feature    5310..5898
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5363..5383)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5781..5792)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5951..6072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5962..5981)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6016..6035
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      6187..6233
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      6263..6399
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    6444..6515
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        6560..6756
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6607..6742
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6770..7564
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6824..6843)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7434..7453
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7575..7799
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(7995..8583)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8075..8094)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(9329..9397,9398..10189)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9547..9566)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      10202..10338
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 tcagcttacc tttttggcag cgatcgcggc tcccgacatc ttggaccatt agctccacag
       61 gtatcttctt ccctctagtg gtcataacag cagcttcagc tacctctcaa ttcaaaaaac
      121 ccctcaagac ccgtttagag gccccaaggg gttatgctat caatcgttgc gttacacaca
      181 caaaaaacca acacacatcc atcttcgatg gatagcgatt ttattatcta actgctgatc
      241 gagtgtagcc agatctagta atcaattacg gggtcattag ttcatagccc atatatggag
      301 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      361 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      421 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      481 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      541 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      601 attaccatgc tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      661 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      721 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      781 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatcgcctgg
      841 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctcccc
      901 tcgaagctta catgtggtac cgagctcgga tcctgagaac ttcagggtga gtctatggga
      961 cccttgatgt tttctttccc cttcttttct atggttaagt tcatgtcata ggaaggggag
     1021 aagtaacagg gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa
     1081 atgctttctt cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct
     1141 ctttctttca gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat
     1201 aacagtgata atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca
     1261 tataaattgt aactgatgta agaggtttca tattgctaat agcagctaca atccagctac
     1321 cattctgctt ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc
     1381 cttttgctaa tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg
     1441 gtctgtgtgc tggcccatca ctttggcaaa ggcggccgcc atgtttgtgt tcctcgttct
     1501 cctgcccctc gtttcttccc aatgcgtgaa cctcacaact agaacgcagc tcccacccgc
     1561 ttacacgaac tcttttactc gtggagtata ctatcccgat aaagtcttta ggtccagtgt
     1621 ccttcattct actcaagacc ttttcctgcc attcttctca aatgtcacct ggttccatgc
     1681 tatctctggc accaacggaa ctaaacgatt tgataatccc gtcttgccat ttaacgatgg
     1741 cgtatacttt gcctcaacgg agaagtccaa cattatccgg ggatggatat ttggcaccac
     1801 cctggactcc aagacacagt ctctcctcat tgtgaacaac gccaccaatg tcgtcatcaa
     1861 agtctgtgaa tttcaatttt gtaacgatcc ctttctcggg gtttatcata agaataacaa
     1921 gtcttggatg gagtcagagt tccgcgttta cagttccgct aacaactgta cttttgagta
     1981 cgtttcccaa ccatttctca tggatcttga agggaagcaa ggcaacttca agaatctccg
     2041 cgaatttgtg tttaagaata tagacggcta cttcaaaatt tatagcaagc ataccccgat
     2101 aaatctggtt cgggatctcc ctcaaggctt ctccgcgttg gaacccctcg tggacttgcc
     2161 gattggcatc aacatcaccc ggttccaaac ccttcttgcc cttcatcgaa gctacctcac
     2221 gcctggcgac agtagtagtg gttggacagc gggcgcagca gcctactatg tgggatattt
     2281 gcaaccacgc acctttcttc tcaaatataa cgaaaacgga acaattaccg atgccgtaga
     2341 ctgtgccctc gatcccctgt ccgaaactaa atgcacactc aagtccttca cagttgagaa
     2401 aggcatatac caaaccagta acttccgcgt ccaacccacc gaatccattg ttcgattccc
     2461 taacatcacc aatttgtgtc ctttcggtga agtatttaat gccactcgat ttgcatcagt
     2521 ttatgcctgg aatcgcaagc gaatatcaaa ttgtgttgca gattatagtg tcctgtataa
     2581 tagcgcatca ttcagcactt ttaagtgtta tggggtcagc cctacgaaat tgaatgactt
     2641 gtgttttacc aacgtttacg cagattcttt cgtcatacgc ggcgacgaag tccgacagat
     2701 tgcaccgggg caaaccggaa agatagcaga ctataattat aagttgccgg atgacttcac
     2761 cggctgcgtt atagcatgga actccaataa tctcgattca aaagtcggcg gcaactataa
     2821 ttatctttac cgcctgtttc gcaagtcaaa tctgaagccc tttgaacgtg acatctccac
     2881 cgaaatttat caagctggca gcaccccatg taatggagtg gaagggttta actgttattt
     2941 tccccttcaa tcatacggat ttcaaccaac atatggggtg ggctatcaac catatcgggt
     3001 ggtagtcttg agctttgagc tgctccacgc accggctaca gtttgcggac cgaagaaaag
     3061 cactaacctg gtgaagaata aatgtgtgaa cttcaacttt aatgggctca ccggaacagg
     3121 ggtgctgaca gaatcaaaca agaaatttct cccttttcaa caatttggta gagacattga
     3181 cgataccaca gatgccgtcc gcgatccgca aacactggaa atcctcgata taacaccctg
     3241 tagcttcggc ggggtcagcg tgataactcc cggaacgaac acatccaacc aagtggctgt
     3301 cctgtatcaa ggtgtcaact gtaccgaagt ccctgtggcg atacacgccg accaactgac
     3361 acccacctgg agagtctact caacaggttc caatgtcttt caaactagag cgggttgtct
     3421 cattggcgct gagcatgtca ataattcata cgaatgtgat attcctattg gggcaggaat
     3481 ctgtgcgtct tatcaaactc aaacgaactc acaccggcgc gctcgctcag tagccagcca
     3541 gtccataatc gcctatacca tgagtctcgg ggctgagaac tccgtggctt attcaaacaa
     3601 ttccatcgca atcccaatta actttaccat tagcgttacc acggaaatct tgcccgtcag
     3661 catgactaag acgagcgtag actgtacgat gtatatctgt ggggattcca ccgagtgtag
     3721 caacctcttg cttcaatacg ggtctttctg tactcaactc aaccgagcat tgaccggaat
     3781 tgcggtcgaa caagacaaga atacccaaga ggtgttcgca caagtaaagc aaatatacaa
     3841 aacgccgcca atcaaagatt ttggcgggtt taattttagc caaatactgc cagacccatc
     3901 taagccttca aaacgcagct ttatcgagga tctgctgttc aacaaagtta cgttggctga
     3961 cgcgggtttc attaagcagt atggggattg tttgggagac attgcggccc gcgaccttat
     4021 ctgtgctcaa aaattcaatg ggttgacagt tctgccccct ctgctcactg atgagatgat
     4081 tgcacagtat acatcagcat tgttggcagg aaccattaca tccggctgga cttttggcgc
     4141 gggtgcggcc cttcaaatac ctttcgctat gcaaatggct tacagattca atgggattgg
     4201 tgtcacgcaa aatgtattgt atgaaaatca gaagctgatc gctaatcaat ttaacagcgc
     4261 gattggcaag atccaagatt cactgtcaag cacagcgtcc gccctcggca aactccaaga
     4321 tgttgtgaat caaaacgccc aggccctgaa taccctcgtg aagcaactga gttctaactt
     4381 cggcgcaatc tccagcgtcc tcaacgacat attggccaga cttgataagg tggaagccga
     4441 ggtgcaaatt gatcggctca tcacaggcag gctccagtca ctccaaactt atgtgacaca
     4501 acagttgatc cgggctgctg agatacgagc aagcgccaac ctggccgcca ctaaaatgtc
     4561 agagtgcgtg ctcgggcagt ccaaacgggt tgacttttgt ggcaagggct atcacttgat
     4621 gtccttcccc caatccgccc cacatggagt ggtctttctc cacgtcactt atgtccctgc
     4681 tcaagagaag aactttacga cagctccagc aatctgtcac gatggcaaag cgcactttcc
     4741 cagggaaggc gttttcgtca gtaatggcac gcattggttt gtgacccaaa ggaatttcta
     4801 tgaaccccaa atcataacca ctcacaatac atttgtctca ggcaactgtg atgtggtcat
     4861 tgggatcgtg aacaacacgg tatatgatcc cctgcaaccc gagttggata gcttcaaaga
     4921 ggaattggat aaatacttta agaatcatac ttcacccgat gttgatctcg gggacatatc
     4981 aggaataaat gcaagtgtcg tcaacattca gaaagagatt gatcgcctta atgaggtcgc
     5041 caagaacttg aatgaatctt tgatcgactt gcaagaactg ggcaaatacg aacaatatat
     5101 taaatggccc tggtacattt ggttgggctt tatcgccgga cttatagcta tcgttatggt
     5161 cacaattatg ttgtgctgta tgactagctg ttgttcctgc ttgaaaggat gttgcagttg
     5221 cggatcttgt tgtaaataaa ggatcctcta gaagttgtct cctcctgcac tgactgactg
     5281 atacaatcga tttctggatc cgcaggccta atcaacctct ggattacaaa atttgtgaaa
     5341 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5401 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5461 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5521 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5581 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5641 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5701 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5761 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5821 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5881 tttgggccgc ctccccgcct gtctagcttg actgactgag atacagcgta ccttcagctc
     5941 acagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     6001 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     6061 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     6121 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtattggccc atctctatcg
     6181 gtatcgtagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgtgcccct
     6241 cgggccggat tgctatctac cggcattggc gcagaaaaaa atgcctgatg cgacgctgcg
     6301 cgtcttatac tcccacatat gccagattca gcaacggata cggcttcccc aacttgccca
     6361 cttccatacg tgtcctcctt accagaaatt tatccttaag gtcgtcagct atcctgcagg
     6421 cgatctctcg atttcgatca agacattcct ttaatggtct tttctggaca ccactagggg
     6481 tcagaagtag ttcatcaaac tttcttccct ccctaatctc attggttacc ttgggctatc
     6541 gaaacttaat taagcgatct gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     6601 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tcgctgacta
     6661 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6721 tgaggaggct tttttggagg cctaggcttt tgcaaaggag gtagccaaca tgattgaaca
     6781 agatggattg cacgcaggtt ctcccgccgc ttgggtggag aggctattcg gctatgactg
     6841 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     6901 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aggacgaggc
     6961 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     7021 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     7081 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     7141 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     7201 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     7261 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg atgcccgacg gcgaggatct
     7321 cgtcgtgacc cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     7381 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     7441 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     7501 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     7561 ctagtatgta agccctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     7621 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     7681 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     7741 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggt
     7801 taattaacca gtcaagtcag ctacttggcg agatcgactt gtctgggttt cgactacgct
     7861 cagaattgcg tcagtcaagt tcgatctggt ccttgctatt gcacccgttc tccgattacg
     7921 agtttcattt aaatcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7981 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8041 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8101 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8161 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8221 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8281 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8341 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8401 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     8461 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8521 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8581 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8641 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8701 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8761 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgca
     8821 tttaaatttc cgaactctcc aaggccctcg tcggaaaatc ttcaaacctt tcgtccgatc
     8881 catcttgcag gctacctctc gaacgaacta tcgcaagtct cttggccggc cttgcgcctt
     8941 ggctattgct tggcagcgcc tatcgccagg tattactcca atcccgaata tccgagatcg
     9001 ggatcacccg agagaagttc aacctacatc ctcaatcccg atctatccga gatccgagga
     9061 atatcgaaat cggggcgcgc ctggtgtacc gagaacgatc ctctcagtgc gagtctcgac
     9121 gatccatatc gttgcttggc agtcagccag tcggaatcca gcttgggacc caggaagtcc
     9181 aatcgtcaga tattgtactc aagcctggtc acggcagcgt accgatctgt ttaaacctag
     9241 atattgatag tctgatcggt caacgtataa tcgagtccta gcttttgcaa acatctatca
     9301 agagacagga tcagcaggag gctttcgcat gagtattcaa catttccgtg tcgcccttat
     9361 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9421 aaaagatgct gaagatcagt tgggtgcgcg agtgggttac atcgaactgg atctcaacag
     9481 cggtaagatc cttgagagtt ttcgccccga agaacgcttt ccaatgatga gcacttttaa
     9541 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     9601 ccgcatacac tattctcaga atgacttggt tgagtattca ccagtcacag aaaagcatct
     9661 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     9721 tgcggccaac ttacttctga caacgattgg aggaccgaag gagctaaccg cttttttgca
     9781 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9841 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacct tgcgtaaact
     9901 attaactggc gaactactta ctctagcttc ccggcaacag ttgatagact ggatggaggc
     9961 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    10021 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    10081 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    10141 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac cgattctagg
    10201 tgcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact cccacatatg
    10261 ccagattcag caacggatac ggcttcccca acttgcccac ttccatacgt gtcctcctta
    10321 ccagaaattt atccttaaga tcccgaatcg tttaaactcg actctggctc tatcgaatct
    10381 ccgtcgtttc gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg gcatcgaatc
    10441 tcgtcagcta tcg
//
