LOCUS       sequence-335485-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 B.1.429 Spike Protein lacking 18 C-terminal amino
            acids for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Garcia-Beltran WF, Lam EC, St Denis K, Nitido AD, Garcia ZH, Hauser 
            BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, 
            Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB
  TITLE     Multiple SARS-CoV-2 variants escape neutralization by 
            vaccine-induced humoral immunity.
  JOURNAL   Cell. 2021 Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013.
  PUBMED    33743213
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 B.1.429
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3878..4466
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3931..3951)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4349..4360)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4519..4640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4530..4549)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4584..4603
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4755..4801
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4831..4967
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5012..5083
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5128..5324
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5175..5310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5338..6132
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5392..5411)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6002..6021
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6143..6367
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6563..7151)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6643..6662)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7897..7965,7966..8757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8115..8134)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8770..8906
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9058..9129
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9134..9180)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9198..9246
                     /note="synthetic polyadenylation signal"
     enhancer        9327..9630
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9632..9834
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9785..9805
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9968..10443
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 tctgtgtgct ggcccatcac tttggcaaag gcggccgcca tgtttgtgtt cctcgttctc
       61 ctgcccctcg tttctatcca atgcgtgaac ctcacaacta gaacgcagct cccacccgct
      121 tacacgaact cttttactcg tggagtatac tatcccgata aagtctttag gtccagtgtc
      181 cttcattcta ctcaagacct tttcctgcca ttcttctcaa atgtcacctg gttccatgct
      241 atccacgttt ctggcaccaa cggaactaaa cgatttgata atcccgtctt gccatttaac
      301 gatggcgtat actttgcctc aacggagaag tccaacatta tccggggatg gatatttggc
      361 accaccctgg actccaagac acagtctctc ctcattgtga acaacgccac caatgtcgtc
      421 atcaaagtct gtgaatttca attttgtaac gatccctttc tcggggttta ttatcataag
      481 aataacaagt cttgcatgga gtcagagttc cgcgtttaca gttccgctaa caactgtact
      541 tttgagtacg tttcccaacc atttctcatg gatcttgaag ggaagcaagg caacttcaag
      601 aatctccgcg aatttgtgtt taagaatata gacggctact tcaaaattta tagcaagcat
      661 accccgataa atctggttcg ggatctccct caaggcttct ccgcgttgga acccctcgtg
      721 gacttgccga ttggcatcaa catcacccgg ttccaaaccc ttcttgccct tcatcgaagc
      781 tacctcacgc ctggcgacag tagtagtggt tggacagcgg gcgcagcagc ctactatgtg
      841 ggatatttgc aaccacgcac ctttcttctc aaatataacg aaaacggaac aattaccgat
      901 gccgtagact gtgccctcga tcccctgtcc gaaactaaat gcacactcaa gtccttcaca
      961 gttgagaaag gcatatacca aaccagtaac ttccgcgtcc aacccaccga atccattgtt
     1021 cgattcccta acatcaccaa tttgtgtcct ttcggtgaag tatttaatgc cactcgattt
     1081 gcatcagttt atgcctggaa tcgcaagcga atatcaaatt gtgttgcaga ttatagtgtc
     1141 ctgtataata gcgcatcatt cagcactttt aagtgttatg gggtcagccc tacgaaattg
     1201 aatgacttgt gttttaccaa cgtttacgca gattctttcg tcatacgcgg cgacgaagtc
     1261 cgacagattg caccggggca aaccggaaag atagcagact ataattataa gttgccggat
     1321 gacttcaccg gctgcgttat agcatggaac tccaataatc tcgattcaaa agtcggcggc
     1381 aactataatt atcgttaccg cctgtttcgc aagtcaaatc tgaagccctt tgaacgtgac
     1441 atctccaccg aaatttatca agctggcagc accccatgta atggagtgga agggtttaac
     1501 tgttattttc cccttcaatc atacggattt caaccaacaa atggggtggg ctatcaacca
     1561 tatcgggtgg tagtcttgag ctttgagctg ctccacgcac cggctacagt ttgcggaccg
     1621 aagaaaagca ctaacctggt gaagaataaa tgtgtgaact tcaactttaa tgggctcacc
     1681 ggaacagggg tgctgacaga atcaaacaag aaatttctcc cttttcaaca atttggtaga
     1741 gacattgccg ataccacaga tgccgtccgc gatccgcaaa cactggaaat cctcgatata
     1801 acaccctgta gcttcggcgg ggtcagcgtg ataactcccg gaacgaacac atccaaccaa
     1861 gtggctgtcc tgtatcaagg tgtcaactgt accgaagtcc ctgtggcgat acacgccgac
     1921 caactgacac ccacctggag agtctactca acaggttcca atgtctttca aactagagcg
     1981 ggttgtctca ttggcgctga gcatgtcaat aattcatacg aatgtgatat tcctattggg
     2041 gcaggaatct gtgcgtctta tcaaactcaa acgaactcac cacggcgcgc tcgctcagta
     2101 gccagccagt ccataatcgc ctataccatg agtctcgggg ctgagaactc cgtggcttat
     2161 tcaaacaatt ccatcgcaat cccaactaac tttaccatta gcgttaccac ggaaatcttg
     2221 cccgtcagca tgactaagac gagcgtagac tgtacgatgt atatctgtgg ggattccacc
     2281 gagtgtagca acctcttgct tcaatacggg tctttctgta ctcaactcaa ccgagcattg
     2341 accggaattg cggtcgaaca agacaagaat acccaagagg tgttcgcaca agtaaagcaa
     2401 atatacaaaa cgccgccaat caaagatttt ggcgggttta attttagcca aatactgcca
     2461 gacccatcta agccttcaaa acgcagcttt atcgaggatc tgctgttcaa caaagttacg
     2521 ttggctgacg cgggtttcat taagcagtat ggggattgtt tgggagacat tgcggcccgc
     2581 gaccttatct gtgctcaaaa attcaatggg ttgacagttc tgccccctct gctcactgat
     2641 gagatgattg cacagtatac atcagcattg ttggcaggaa ccattacatc cggctggact
     2701 tttggcgcgg gtgcggccct tcaaatacct ttcgctatgc aaatggctta cagattcaat
     2761 gggattggtg tcacgcaaaa tgtattgtat gaaaatcaga agctgatcgc taatcaattt
     2821 aacagcgcga ttggcaagat ccaagattca ctgtcaagca cagcgtccgc cctcggcaaa
     2881 ctccaagatg ttgtgaatca aaacgcccag gccctgaata ccctcgtgaa gcaactgagt
     2941 tctaacttcg gcgcaatctc cagcgtcctc aacgacatat tgtccagact tgataaggtg
     3001 gaagccgagg tgcaaattga tcggctcatc acaggcaggc tccagtcact ccaaacttat
     3061 gtgacacaac agttgatccg ggctgctgag atacgagcaa gcgccaacct ggccgccact
     3121 aaaatgtcag agtgcgtgct cgggcagtcc aaacgggttg acttttgtgg caagggctat
     3181 cacttgatgt ccttccccca atccgcccca catggagtgg tctttctcca cgtcacttat
     3241 gtccctgctc aagagaagaa ctttacgaca gctccagcaa tctgtcacga tggcaaagcg
     3301 cactttccca gggaaggcgt tttcgtcagt aatggcacgc attggtttgt gacccaaagg
     3361 aatttctatg aaccccaaat cataaccact gacaatacat ttgtctcagg caactgtgat
     3421 gtggtcattg ggatcgtgaa caacacggta tatgatcccc tgcaacccga gttggatagc
     3481 ttcaaagagg aattggataa atactttaag aatcatactt cacccgatgt tgatctcggg
     3541 gacatatcag gaataaatgc aagtgtcgtc aacattcaga aagagattga tcgccttaat
     3601 gaggtcgcca agaacttgaa tgaatctttg atcgacttgc aagaactggg caaatacgaa
     3661 caatatatta aatggccctg gtacatttgg ttgggcttta tcgccggact tatagctatc
     3721 gttatggtca caattatgtt gtgctgtatg actagctgtt gttcctgctt gaaaggatgt
     3781 tgcagttgcg gatcttgttg taaataaagg atcctctaga agttgtctcc tcctgcactg
     3841 actgactgat acaatcgatt tctggatccg caggcctaat caacctctgg attacaaaat
     3901 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3961 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4021 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4081 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4141 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4201 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4261 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     4321 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4381 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4441 gatctccctt tgggccgcct ccccgcctgt ctagcttgac tgactgagat acagcgtacc
     4501 ttcagctcac agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     4561 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     4621 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     4681 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt attggcccat
     4741 ctctatcggt atcgtagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     4801 gtgcccctcg ggccggattg ctatctaccg gcattggcgc agaaaaaaat gcctgatgcg
     4861 acgctgcgcg tcttatactc ccacatatgc cagattcagc aacggatacg gcttccccaa
     4921 cttgcccact tccatacgtg tcctccttac cagaaattta tccttaaggt cgtcagctat
     4981 cctgcaggcg atctctcgat ttcgatcaag acattccttt aatggtcttt tctggacacc
     5041 actaggggtc agaagtagtt catcaaactt tcttccctcc ctaatctcat tggttacctt
     5101 gggctatcga aacttaatta agcgatctgc atctcaatta gtcagcaacc atagtcccgc
     5161 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatc
     5221 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     5281 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaggaggt agccaacatg
     5341 attgaacaag atggattgca cgcaggttct cccgccgctt gggtggagag gctattcggc
     5401 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5461 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactccag
     5521 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5581 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5641 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5701 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5761 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5821 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcggat gcccgacggc
     5881 gaggatctcg tcgtgaccca cggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5941 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     6001 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     6061 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     6121 gagttcttct agtatgtaag ccctgtgcct tctagttgcc agccatctgt tgtttgcccc
     6181 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     6241 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6301 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     6361 tctatggtta attaaccagt caagtcagct acttggcgag atcgacttgt ctgggtttcg
     6421 actacgctca gaattgcgtc agtcaagttc gatctggtcc ttgctattgc acccgttctc
     6481 cgattacgag tttcatttaa atcatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6541 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6601 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6661 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6721 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6781 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6841 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6901 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6961 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7021 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7081 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7141 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7201 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7261 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7321 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7381 tagttgcatt taaatttccg aactctccaa ggccctcgtc ggaaaatctt caaacctttc
     7441 gtccgatcca tcttgcaggc tacctctcga acgaactatc gcaagtctct tggccggcct
     7501 tgcgccttgg ctattgcttg gcagcgccta tcgccaggta ttactccaat cccgaatatc
     7561 cgagatcggg atcacccgag agaagttcaa cctacatcct caatcccgat ctatccgaga
     7621 tccgaggaat atcgaaatcg gggcgcgcct ggtgtaccga gaacgatcct ctcagtgcga
     7681 gtctcgacga tccatatcgt tgcttggcag tcagccagtc ggaatccagc ttgggaccca
     7741 ggaagtccaa tcgtcagata ttgtactcaa gcctggtcac ggcagcgtac cgatctgttt
     7801 aaacctagat attgatagtc tgatcggtca acgtataatc gagtcctagc ttttgcaaac
     7861 atctatcaag agacaggatc agcaggaggc tttcgcatga gtattcaaca tttccgtgtc
     7921 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7981 gtgaaagtaa aagatgctga agatcagttg ggtgcgcgag tgggttacat cgaactggat
     8041 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgctttcc aatgatgagc
     8101 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     8161 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtattcacc agtcacagaa
     8221 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8281 gataacactg cggccaactt acttctgaca acgattggag gaccgaagga gctaaccgct
     8341 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8401 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaaccttg
     8461 cgtaaactat taactggcga actacttact ctagcttccc ggcaacagtt gatagactgg
     8521 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8581 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8641 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8701 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaaccg
     8761 attctaggtg cattggcgca gaaaaaaatg cctgatgcga cgctgcgcgt cttatactcc
     8821 cacatatgcc agattcagca acggatacgg cttccccaac ttgcccactt ccatacgtgt
     8881 cctccttacc agaaatttat ccttaagatc ccgaatcgtt taaactcgac tctggctcta
     8941 tcgaatctcc gtcgtttcga gcttacgcga acagccgtgg cgctcatttg ctcgtcgggc
     9001 atcgaatctc gtcagctatc gtcagcttac ctttttggca gcgatcgcgg ctcccgacat
     9061 cttggaccat tagctccaca ggtatcttct tccctctagt ggtcataaca gcagcttcag
     9121 ctacctctca attcaaaaaa cccctcaaga cccgtttaga ggccccaagg ggttatgcta
     9181 tcaatcgttg cgttacacac acaaaaaacc aacacacatc catcttcgat ggatagcgat
     9241 tttattatct aactgctgat cgagtgtagc cagatctagt aatcaattac ggggtcatta
     9301 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     9361 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     9421 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     9481 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     9541 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     9601 atctacgtat tagtcatcgc tattaccatg ctgatgcggt tttggcagta catcaatggg
     9661 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     9721 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     9781 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     9841 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     9901 cgggaccgat ccagcctccc ctcgaagctt acatgtggta ccgagctcgg atcctgagaa
     9961 cttcagggtg agtctatggg acccttgatg ttttctttcc ccttcttttc tatggttaag
    10021 ttcatgtcat aggaagggga gaagtaacag ggtacacata ttgaccaaat cagggtaatt
    10081 ttgcatttgt aattttaaaa aatgctttct tcttttaata tacttttttg tttatcttat
    10141 ttctaatact ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct
    10201 ctttgcacca ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt
    10261 ctgcatataa atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa
    10321 tagcagctac aatccagcta ccattctgct tttattttat ggttgggata aggctggatt
    10381 attctgagtc caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca
    10441 cagctcctgg gcaacgtgct gg
//
