LOCUS       sequence-335486-       10453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 B.1.351v2 Spike Protein lacking 18 C-terminal 
            amino acids for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Garcia-Beltran WF, Lam EC, St Denis K, Nitido AD, Garcia ZH, Hauser 
            BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, 
            Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB
  TITLE     Multiple SARS-CoV-2 variants escape neutralization by 
            vaccine-induced humoral immunity.
  JOURNAL   Cell. 2021 Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013.
  PUBMED    33743213
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 B.1.351v2
FEATURES             Location/Qualifiers
     source          1..10453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3855..4443
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3908..3928)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4326..4337)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4496..4617
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4507..4526)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4561..4580
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4732..4778
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4808..4944
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4989..5060
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5105..5301
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5152..5287
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5315..6109
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5369..5388)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5979..5998
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6120..6344
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6540..7128)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6620..6639)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7874..7942,7943..8734)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8092..8111)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8747..8883
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9035..9106
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9111..9157)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9175..9223
                     /note="synthetic polyadenylation signal"
     enhancer        9304..9607
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9609..9811
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9762..9782
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9945..10420
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 catcactttg gcaaaggcgg ccgccatgtt tgtgttcctc gttctcctgc ccctcgtttc
       61 ttcccaatgc gtgaacttca caactagaac gcagctccca cccgcttaca cgaactcttt
      121 tactcgtgga gtatactatc ccgataaagt ctttaggtcc agtgtccttc attctactca
      181 agaccttttc ctgccattct tctcaaatgt cacctggttc catgctatcc acgtttctgg
      241 caccaacgga actaaacgat ttgctaatcc cgtcttgcca tttaacgatg gcgtatactt
      301 tgcctcaacg gagaagtcca acattatccg gggatggata tttggcacca ccctggactc
      361 caagacacag tctctcctca ttgtgaacaa cgccaccaat gtcgtcatca aagtctgtga
      421 atttcaattt tgtaacgatc cctttctcgg ggtttattat cataagaata acaagtcttg
      481 gatggagtca gagttccgcg tttacagttc cgctaacaac tgtacttttg agtacgtttc
      541 ccaaccattt ctcatggatc ttgaagggaa gcaaggcaac ttcaagaatc tccgcgaatt
      601 tgtgtttaag aatatagacg gctacttcaa aatttatagc aagcataccc cgataaatct
      661 ggttcggggt ctccctcaag gcttctccgc gttggaaccc ctcgtggact tgccgattgg
      721 catcaacatc acccggttcc aaacccttca tcgaagctac ctcacgcctg gcgacagtag
      781 tagtggttgg acagcgggcg cagcagccta ctatgtggga tatttgcaac cacgcacctt
      841 tcttctcaaa tataacgaaa acggaacaat taccgatgcc gtagactgtg ccctcgatcc
      901 cctgtccgaa actaaatgca cactcaagtc cttcacagtt gagaaaggca tataccaaac
      961 cagtaacttc cgcgtccaac ccaccgaatc cattgttcga ttccctaaca tcaccaattt
     1021 gtgtcctttc ggtgaagtat ttaatgccac tcgatttgca tcagtttatg cctggaatcg
     1081 caagcgaata tcaaattgtg ttgcagatta tagtgtcctg tataatagcg catcattcag
     1141 cacttttaag tgttatgggg tcagccctac gaaattgaat gacttgtgtt ttaccaacgt
     1201 ttacgcagat tctttcgtca tacgcggcga cgaagtccga cagattgcac cggggcaaac
     1261 cggaaatata gcagactata attataagtt gccggatgac ttcaccggct gcgttatagc
     1321 atggaactcc aataatctcg attcaaaagt cggcggcaac tataattatc tttaccgcct
     1381 gtttcgcaag tcaaatctga agccctttga acgtgacatc tccaccgaaa tttatcaagc
     1441 tggcagcacc ccatgtaatg gagtgaaagg gtttaactgt tattttcccc ttcaatcata
     1501 cggatttcaa ccaacatatg gggtgggcta tcaaccatat cgggtggtag tcttgagctt
     1561 tgagctgctc cacgcaccgg ctacagtttg cggaccgaag aaaagcacta acctggtgaa
     1621 gaataaatgt gtgaacttca actttaatgg gctcaccgga acaggggtgc tgacagaatc
     1681 aaacaagaaa tttctccctt ttcaacaatt tggtagagac attgccgata ccacagatgc
     1741 cgtccgcgat ccgcaaacac tggaaatcct cgatataaca ccctgtagct tcggcggggt
     1801 cagcgtgata actcccggaa cgaacacatc caaccaagtg gctgtcctgt atcaaggtgt
     1861 caactgtacc gaagtccctg tggcgataca cgccgaccaa ctgacaccca cctggagagt
     1921 ctactcaaca ggttccaatg tctttcaaac tagagcgggt tgtctcattg gcgctgagca
     1981 tgtcaataat tcatacgaat gtgatattcc tattggggca ggaatctgtg cgtcttatca
     2041 aactcaaacg aactcaccac ggcgcgctcg ctcagtagcc agccagtcca taatcgccta
     2101 taccatgagt ctcggggttg agaactccgt ggcttattca aacaattcca tcgcaatccc
     2161 aactaacttt accattagcg ttaccacgga aatcttgccc gtcagcatga ctaagacgag
     2221 cgtagactgt acgatgtata tctgtgggga ttccaccgag tgtagcaacc tcttgcttca
     2281 atacgggtct ttctgtactc aactcaaccg agcattgacc ggaattgcgg tcgaacaaga
     2341 caagaatacc caagaggtgt tcgcacaagt aaagcaaata tacaaaacgc cgccaatcaa
     2401 agattttggc gggtttaatt ttagccaaat actgccagac ccatctaagc cttcaaaacg
     2461 cagctttatc gaggatctgc tgttcaacaa agttacgttg gctgacgcgg gtttcattaa
     2521 gcagtatggg gattgtttgg gagacattgc ggcccgcgac cttatctgtg ctcaaaaatt
     2581 caatgggttg acagttctgc cccctctgct cactgatgag atgattgcac agtatacatc
     2641 agcattgttg gcaggaacca ttacatccgg ctggactttt ggcgcgggtg cggcccttca
     2701 aatacctttc gctatgcaaa tggcttacag attcaatggg attggtgtca cgcaaaatgt
     2761 attgtatgaa aatcagaagc tgatcgctaa tcaatttaac agcgcgattg gcaagatcca
     2821 agattcactg tcaagcacag cgtccgccct cggcaaactc caagatgttg tgaatcaaaa
     2881 cgcccaggcc ctgaataccc tcgtgaagca actgagttct aacttcggcg caatctccag
     2941 cgtcctcaac gacatattgt ccagacttga taaggtggaa gccgaggtgc aaattgatcg
     3001 gctcatcaca ggcaggctcc agtcactcca aacttatgtg acacaacagt tgatccgggc
     3061 tgctgagata cgagcaagcg ccaacctggc cgccactaaa atgtcagagt gcgtgctcgg
     3121 gcagtccaaa cgggttgact tttgtggcaa gggctatcac ttgatgtcct tcccccaatc
     3181 cgccccacat ggagtggtct ttctccacgt cacttatgtc cctgctcaag agaagaactt
     3241 tacgacagct ccagcaatct gtcacgatgg caaagcgcac tttcccaggg aaggcgtttt
     3301 cgtcagtaat ggcacgcatt ggtttgtgac ccaaaggaat ttctatgaac cccaaatcat
     3361 aaccactgac aatacatttg tctcaggcaa ctgtgatgtg gtcattggga tcgtgaacaa
     3421 cacggtatat gatcccctgc aacccgagtt ggatagcttc aaagaggaat tggataaata
     3481 ctttaagaat catacttcac ccgatgttga tctcggggac atatcaggaa taaatgcaag
     3541 tgtcgtcaac attcagaaag agattgatcg ccttaatgag gtcgccaaga acttgaatga
     3601 atctttgatc gacttgcaag aactgggcaa atacgaacaa tatattaaat ggccctggta
     3661 catttggttg ggctttatcg ccggacttat agctatcgtt atggtcacaa ttatgttgtg
     3721 ctgtatgact agctgttgtt cctgcttgaa aggatgttgc agttgcggat cttgttgtaa
     3781 ataaaggatc ctctagaagt tgtctcctcc tgcactgact gactgataca atcgatttct
     3841 ggatccgcag gcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3901 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3961 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4021 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4081 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4141 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4201 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     4261 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4321 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4381 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4441 cgcctgtcta gcttgactga ctgagataca gcgtaccttc agctcacaga catgataaga
     4501 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     4561 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     4621 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     4681 agcaagtaaa acctctacaa atgtggtatt ggcccatctc tatcggtatc gtagcataac
     4741 cccttggggc ctctaaacgg gtcttgaggg gttttttgtg cccctcgggc cggattgcta
     4801 tctaccggca ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca
     4861 catatgccag attcagcaac ggatacggct tccccaactt gcccacttcc atacgtgtcc
     4921 tccttaccag aaatttatcc ttaaggtcgt cagctatcct gcaggcgatc tctcgatttc
     4981 gatcaagaca ttcctttaat ggtcttttct ggacaccact aggggtcaga agtagttcat
     5041 caaactttct tccctcccta atctcattgg ttaccttggg ctatcgaaac ttaattaagc
     5101 gatctgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5161 ctaactccgc ccagttccgc ccattctccg ccccatcgct gactaatttt ttttatttat
     5221 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5281 ggaggcctag gcttttgcaa aggaggtagc caacatgatt gaacaagatg gattgcacgc
     5341 aggttctccc gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     5401 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     5461 caagaccgac ctgtccggtg ccctgaatga actccaggac gaggcagcgc ggctatcgtg
     5521 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     5581 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     5641 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     5701 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5761 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5821 actgttcgcc aggctcaagg cgcggatgcc cgacggcgag gatctcgtcg tgacccacgg
     5881 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     5941 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     6001 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     6061 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctagt atgtaagccc
     6121 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     6181 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     6241 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     6301 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggttaatt aaccagtcaa
     6361 gtcagctact tggcgagatc gacttgtctg ggtttcgact acgctcagaa ttgcgtcagt
     6421 caagttcgat ctggtccttg ctattgcacc cgttctccga ttacgagttt catttaaatc
     6481 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6541 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6601 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6661 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6721 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6781 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6841 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6901 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6961 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7021 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7081 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7141 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7201 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7261 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7321 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcatttaa atttccgaac
     7381 tctccaaggc cctcgtcgga aaatcttcaa acctttcgtc cgatccatct tgcaggctac
     7441 ctctcgaacg aactatcgca agtctcttgg ccggccttgc gccttggcta ttgcttggca
     7501 gcgcctatcg ccaggtatta ctccaatccc gaatatccga gatcgggatc acccgagaga
     7561 agttcaacct acatcctcaa tcccgatcta tccgagatcc gaggaatatc gaaatcgggg
     7621 cgcgcctggt gtaccgagaa cgatcctctc agtgcgagtc tcgacgatcc atatcgttgc
     7681 ttggcagtca gccagtcgga atccagcttg ggacccagga agtccaatcg tcagatattg
     7741 tactcaagcc tggtcacggc agcgtaccga tctgtttaaa cctagatatt gatagtctga
     7801 tcggtcaacg tataatcgag tcctagcttt tgcaaacatc tatcaagaga caggatcagc
     7861 aggaggcttt cgcatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7921 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7981 tcagttgggt gcgcgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8041 gagttttcgc cccgaagaac gctttccaat gatgagcact tttaaagttc tgctatgtgg
     8101 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8161 tcagaatgac ttggttgagt attcaccagt cacagaaaag catcttacgg atggcatgac
     8221 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8281 tctgacaacg attggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8341 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8401 tgacaccacg atgcctgtag caatggcaac aaccttgcgt aaactattaa ctggcgaact
     8461 acttactcta gcttcccggc aacagttgat agactggatg gaggcggata aagttgcagg
     8521 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8581 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8641 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8701 tgagataggt gcctcactga ttaagcattg gtaaccgatt ctaggtgcat tggcgcagaa
     8761 aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac atatgccaga ttcagcaacg
     8821 gatacggctt ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct
     8881 taagatcccg aatcgtttaa actcgactct ggctctatcg aatctccgtc gtttcgagct
     8941 tacgcgaaca gccgtggcgc tcatttgctc gtcgggcatc gaatctcgtc agctatcgtc
     9001 agcttacctt tttggcagcg atcgcggctc ccgacatctt ggaccattag ctccacaggt
     9061 atcttcttcc ctctagtggt cataacagca gcttcagcta cctctcaatt caaaaaaccc
     9121 ctcaagaccc gtttagaggc cccaaggggt tatgctatca atcgttgcgt tacacacaca
     9181 aaaaaccaac acacatccat cttcgatgga tagcgatttt attatctaac tgctgatcga
     9241 gtgtagccag atctagtaat caattacggg gtcattagtt catagcccat atatggagtt
     9301 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     9361 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     9421 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     9481 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     9541 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     9601 taccatgctg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     9661 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     9721 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     9781 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tcgcctggag
     9841 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctcccctc
     9901 gaagcttaca tgtggtaccg agctcggatc ctgagaactt cagggtgagt ctatgggacc
     9961 cttgatgttt tctttcccct tcttttctat ggttaagttc atgtcatagg aaggggagaa
    10021 gtaacagggt acacatattg accaaatcag ggtaattttg catttgtaat tttaaaaaat
    10081 gctttcttct tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct
    10141 ttctttcagg gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa
    10201 cagtgataat ttctgggtta aggcaatagc aatatttctg catataaata tttctgcata
    10261 taaattgtaa ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca
    10321 ttctgctttt attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct
    10381 tttgctaatc atgttcatac ctcttatctt cctcccacag ctcctgggca acgtgctggt
    10441 ctgtgtgctg gcc
//
