LOCUS       sequence-335487-       10453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal 
            amino acids for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Garcia-Beltran WF, Lam EC, St Denis K, Nitido AD, Garcia ZH, Hauser 
            BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, 
            Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB
  TITLE     Multiple SARS-CoV-2 variants escape neutralization by 
            vaccine-induced humoral immunity.
  JOURNAL   Cell. 2021 Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013.
  PUBMED    33743213
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 B.1.351v3
FEATURES             Location/Qualifiers
     source          1..10453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3880..4468
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3933..3953)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4351..4362)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4521..4642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4532..4551)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4586..4605
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4757..4803
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4833..4969
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5014..5085
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5130..5326
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5177..5312
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5340..6134
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5394..5413)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6004..6023
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6145..6369
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6565..7153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6645..6664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7899..7967,7968..8759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8117..8136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8772..8908
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9060..9131
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9136..9182)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9200..9248
                     /note="synthetic polyadenylation signal"
     enhancer        9329..9632
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9634..9836
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9787..9807
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9970..10445
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 caacgtgctg gtctgtgtgc tggcccatca ctttggcaaa ggcggccgcc atgtttgtgt
       61 tcctcgttct cctgcccctc gtttcttccc aatgcgtgaa cctcacaact agaacgcagc
      121 tcccacccgc ttacacgaac tcttttactc gtggagtata ctatcccgat aaagtcttta
      181 ggtccagtgt ccttcattct actcaagacc ttttcctgcc attcttctca aatgtcacct
      241 ggttccatgc tatccacgtt tctggcacca acggaactaa acgatttgct aatcccgtct
      301 tgccatttaa cgatggcgta tactttgcct caacggagaa gtccaacatt atccggggat
      361 ggatatttgg caccaccctg gactccaaga cacagtctct cctcattgtg aacaacgcca
      421 ccaatgtcgt catcaaagtc tgtgaatttc aattttgtaa cgatcccttt ctcggggttt
      481 attatcataa gaataacaag tcttggatgg agtcagagtt ccgcgtttac agttccgcta
      541 acaactgtac ttttgagtac gtttcccaac catttctcat ggatcttgaa gggaagcaag
      601 gcaacttcaa gaatctccgc gaatttgtgt ttaagaatat agacggctac ttcaaaattt
      661 atagcaagca taccccgata aatctggttc gggatctccc tcaaggcttc tccgcgttgg
      721 aacccctcgt ggacttgccg attggcatca acatcacccg gttccaaacc cttcatataa
      781 gctacctcac gcctggcgac agtagtagtg gttggacagc gggcgcagca gcctactatg
      841 tgggatattt gcaaccacgc acctttcttc tcaaatataa cgaaaacgga acaattaccg
      901 atgccgtaga ctgtgccctc gatcccctgt ccgaaactaa atgcacactc aagtccttca
      961 cagttgagaa aggcatatac caaaccagta acttccgcgt ccaacccacc gaatccattg
     1021 ttcgattccc taacatcacc aatttgtgtc ctttcggtga agtatttaat gccactcgat
     1081 ttgcatcagt ttatgcctgg aatcgcaagc gaatatcaaa ttgtgttgca gattatagtg
     1141 tcctgtataa tagcgcatca ttcagcactt ttaagtgtta tggggtcagc cctacgaaat
     1201 tgaatgactt gtgttttacc aacgtttacg cagattcttt cgtcatacgc ggcgacgaag
     1261 tccgacagat tgcaccgggg caaaccggaa atatagcaga ctataattat aagttgccgg
     1321 atgacttcac cggctgcgtt atagcatgga actccaataa tctcgattca aaagtcggcg
     1381 gcaactataa ttatctttac cgcctgtttc gcaagtcaaa tctgaagccc tttgaacgtg
     1441 acatctccac cgaaatttat caagctggca gcaccccatg taatggagtg aaagggttta
     1501 actgttattt tccccttcaa tcatacggat ttcaaccaac atatggggtg ggctatcaac
     1561 catatcgggt ggtagtcttg agctttgagc tgctccacgc accggctaca gtttgcggac
     1621 cgaagaaaag cactaacctg gtgaagaata aatgtgtgaa cttcaacttt aatgggctca
     1681 ccggaacagg ggtgctgaca gaatcaaaca agaaatttct cccttttcaa caatttggta
     1741 gagacattgc cgataccaca gatgccgtcc gcgatccgca aacactggaa atcctcgata
     1801 taacaccctg tagcttcggc ggggtcagcg tgataactcc cggaacgaac acatccaacc
     1861 aagtggctgt cctgtatcaa ggtgtcaact gtaccgaagt ccctgtggcg atacacgccg
     1921 accaactgac acccacctgg agagtctact caacaggttc caatgtcttt caaactagag
     1981 cgggttgtct cattggcgct gagcatgtca ataattcata cgaatgtgat attcctattg
     2041 gggcaggaat ctgtgcgtct tatcaaactc aaacgaactc accacggcgc gctcgctcag
     2101 tagccagcca gtccataatc gcctatacca tgagtctcgg ggttgagaac tccgtggctt
     2161 attcaaacaa ttccatcgca atcccaacta actttaccat tagcgttacc acggaaatct
     2221 tgcccgtcag catgactaag acgagcgtag actgtacgat gtatatctgt ggggattcca
     2281 ccgagtgtag caacctcttg cttcaatacg ggtctttctg tactcaactc aaccgagcat
     2341 tgaccggaat tgcggtcgaa caagacaaga atacccaaga ggtgttcgca caagtaaagc
     2401 aaatatacaa aacgccgcca atcaaagatt ttggcgggtt taattttagc caaatactgc
     2461 cagacccatc taagccttca aaacgcagct ttatcgagga tctgctgttc aacaaagtta
     2521 cgttggctga cgcgggtttc attaagcagt atggggattg tttgggagac attgcggccc
     2581 gcgaccttat ctgtgctcaa aaattcaatg ggttgacagt tctgccccct ctgctcactg
     2641 atgagatgat tgcacagtat acatcagcat tgttggcagg aaccattaca tccggctgga
     2701 cttttggcgc gggtgcggcc cttcaaatac ctttcgctat gcaaatggct tacagattca
     2761 atgggattgg tgtcacgcaa aatgtattgt atgaaaatca gaagctgatc gctaatcaat
     2821 ttaacagcgc gattggcaag atccaagatt cactgtcaag cacagcgtcc gccctcggca
     2881 aactccaaga tgttgtgaat caaaacgccc aggccctgaa taccctcgtg aagcaactga
     2941 gttctaactt cggcgcaatc tccagcgtcc tcaacgacat attgtccaga cttgataagg
     3001 tggaagccga ggtgcaaatt gatcggctca tcacaggcag gctccagtca ctccaaactt
     3061 atgtgacaca acagttgatc cgggctgctg agatacgagc aagcgccaac ctggccgcca
     3121 ctaaaatgtc agagtgcgtg ctcgggcagt ccaaacgggt tgacttttgt ggcaagggct
     3181 atcacttgat gtccttcccc caatccgccc cacatggagt ggtctttctc cacgtcactt
     3241 atgtccctgc tcaagagaag aactttacga cagctccagc aatctgtcac gatggcaaag
     3301 cgcactttcc cagggaaggc gttttcgtca gtaatggcac gcattggttt gtgacccaaa
     3361 ggaatttcta tgaaccccaa atcataacca ctgacaatac atttgtctca ggcaactgtg
     3421 atgtggtcat tgggatcgtg aacaacacgg tatatgatcc cctgcaaccc gagttggata
     3481 gcttcaaaga ggaattggat aaatacttta agaatcatac ttcacccgat gttgatctcg
     3541 gggacatatc aggaataaat gcaagtgtcg tcaacattca gaaagagatt gatcgcctta
     3601 atgaggtcgc caagaacttg aatgaatctt tgatcgactt gcaagaactg ggcaaatacg
     3661 aacaatatat taaatggccc tggtacattt ggttgggctt tatcgccgga cttatagcta
     3721 tcgttatggt cacaattatg ttgtgctgta tgactagctg ttgttcctgc ttgaaaggat
     3781 gttgcagttg cggatcttgt tgtaaataaa ggatcctcta gaagttgtct cctcctgcac
     3841 tgactgactg atacaatcga tttctggatc cgcaggccta atcaacctct ggattacaaa
     3901 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3961 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4021 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4081 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4141 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4201 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4261 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     4321 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4381 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4441 cggatctccc tttgggccgc ctccccgcct gtctagcttg actgactgag atacagcgta
     4501 ccttcagctc acagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     4561 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     4621 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     4681 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtattggccc
     4741 atctctatcg gtatcgtagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     4801 ttgtgcccct cgggccggat tgctatctac cggcattggc gcagaaaaaa atgcctgatg
     4861 cgacgctgcg cgtcttatac tcccacatat gccagattca gcaacggata cggcttcccc
     4921 aacttgccca cttccatacg tgtcctcctt accagaaatt tatccttaag gtcgtcagct
     4981 atcctgcagg cgatctctcg atttcgatca agacattcct ttaatggtct tttctggaca
     5041 ccactagggg tcagaagtag ttcatcaaac tttcttccct ccctaatctc attggttacc
     5101 ttgggctatc gaaacttaat taagcgatct gcatctcaat tagtcagcaa ccatagtccc
     5161 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5221 tcgctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5281 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaggag gtagccaaca
     5341 tgattgaaca agatggattg cacgcaggtt ctcccgccgc ttgggtggag aggctattcg
     5401 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     5461 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc
     5521 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     5581 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     5641 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     5701 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     5761 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     5821 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg atgcccgacg
     5881 gcgaggatct cgtcgtgacc cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5941 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     6001 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     6061 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     6121 acgagttctt ctagtatgta agccctgtgc cttctagttg ccagccatct gttgtttgcc
     6181 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6241 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     6301 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     6361 gctctatggt taattaacca gtcaagtcag ctacttggcg agatcgactt gtctgggttt
     6421 cgactacgct cagaattgcg tcagtcaagt tcgatctggt ccttgctatt gcacccgttc
     6481 tccgattacg agtttcattt aaatcatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6541 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6601 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6661 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6721 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6781 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6841 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6901 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6961 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7021 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7081 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7141 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7201 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7261 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7321 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7381 catagttgca tttaaatttc cgaactctcc aaggccctcg tcggaaaatc ttcaaacctt
     7441 tcgtccgatc catcttgcag gctacctctc gaacgaacta tcgcaagtct cttggccggc
     7501 cttgcgcctt ggctattgct tggcagcgcc tatcgccagg tattactcca atcccgaata
     7561 tccgagatcg ggatcacccg agagaagttc aacctacatc ctcaatcccg atctatccga
     7621 gatccgagga atatcgaaat cggggcgcgc ctggtgtacc gagaacgatc ctctcagtgc
     7681 gagtctcgac gatccatatc gttgcttggc agtcagccag tcggaatcca gcttgggacc
     7741 caggaagtcc aatcgtcaga tattgtactc aagcctggtc acggcagcgt accgatctgt
     7801 ttaaacctag atattgatag tctgatcggt caacgtataa tcgagtccta gcttttgcaa
     7861 acatctatca agagacagga tcagcaggag gctttcgcat gagtattcaa catttccgtg
     7921 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7981 tggtgaaagt aaaagatgct gaagatcagt tgggtgcgcg agtgggttac atcgaactgg
     8041 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgcttt ccaatgatga
     8101 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8161 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtattca ccagtcacag
     8221 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8281 gtgataacac tgcggccaac ttacttctga caacgattgg aggaccgaag gagctaaccg
     8341 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8401 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacct
     8461 tgcgtaaact attaactggc gaactactta ctctagcttc ccggcaacag ttgatagact
     8521 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8581 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8641 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8701 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8761 cgattctagg tgcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact
     8821 cccacatatg ccagattcag caacggatac ggcttcccca acttgcccac ttccatacgt
     8881 gtcctcctta ccagaaattt atccttaaga tcccgaatcg tttaaactcg actctggctc
     8941 tatcgaatct ccgtcgtttc gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg
     9001 gcatcgaatc tcgtcagcta tcgtcagctt acctttttgg cagcgatcgc ggctcccgac
     9061 atcttggacc attagctcca caggtatctt cttccctcta gtggtcataa cagcagcttc
     9121 agctacctct caattcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
     9181 tatcaatcgt tgcgttacac acacaaaaaa ccaacacaca tccatcttcg atggatagcg
     9241 attttattat ctaactgctg atcgagtgta gccagatcta gtaatcaatt acggggtcat
     9301 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     9361 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     9421 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     9481 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     9541 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     9601 acatctacgt attagtcatc gctattacca tgctgatgcg gttttggcag tacatcaatg
     9661 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     9721 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     9781 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
     9841 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     9901 accgggaccg atccagcctc ccctcgaagc ttacatgtgg taccgagctc ggatcctgag
     9961 aacttcaggg tgagtctatg ggacccttga tgttttcttt ccccttcttt tctatggtta
    10021 agttcatgtc ataggaaggg gagaagtaac agggtacaca tattgaccaa atcagggtaa
    10081 ttttgcattt gtaattttaa aaaatgcttt cttcttttaa tatacttttt tgtttatctt
    10141 atttctaata ctttccctaa tctctttctt tcagggcaat aatgatacaa tgtatcatgc
    10201 ctctttgcac cattctaaag aataacagtg ataatttctg ggttaaggca atagcaatat
    10261 ttctgcatat aaatatttct gcatataaat tgtaactgat gtaagaggtt tcatattgct
    10321 aatagcagct acaatccagc taccattctg cttttatttt atggttggga taaggctgga
    10381 ttattctgag tccaagctag gcccttttgc taatcatgtt catacctctt atcttcctcc
    10441 cacagctcct ggg
//
