LOCUS       sequence-335488-       10408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV Spike Protein lacking 18 C-terminal amino acids for
            pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Garcia-Beltran WF, Lam EC, St Denis K, Nitido AD, Garcia ZH, Hauser 
            BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, 
            Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB
  TITLE     Multiple SARS-CoV-2 variants escape neutralization by 
            vaccine-induced humoral immunity.
  JOURNAL   Cell. 2021 Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013.
  PUBMED    33743213
REFERENCE   2  (bases 1 to 10408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013."
COMMENT     Sequence Label: pTwist-SARS-CoV Delta-18
FEATURES             Location/Qualifiers
     source          1..10408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3820..4408
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3873..3893)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4291..4302)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4461..4582
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4472..4491)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4526..4545
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4697..4743
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4773..4909
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4954..5025
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5070..5266
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5117..5252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5280..6074
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5334..5353)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5944..5963
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6085..6309
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6505..7093)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6585..6604)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7839..7907,7908..8699)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8057..8076)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8712..8848
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9000..9071
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9076..9122)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9140..9188
                     /note="synthetic polyadenylation signal"
     enhancer        9269..9572
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9574..9776
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9727..9747
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9910..10385
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 tgtgctggcc catcactttg gcaaaggcgg ccgccatgtt tatattcctg ctgtttctga
       61 ccttgacatc cgggagcgat ctcgataggt gtactacatt cgacgatgtc caagccccca
      121 actatactca acacacatca tcaatgcgcg gtgtttatta tcctgatgaa atctttagaa
      181 gcgatactct ctatttgacc caagacctgt ttcttccctt ttactccaac gtcaccggct
      241 tccatactat taaccacact ttcggcaatc ccgtcattcc gttcaaggat ggaatttatt
      301 tcgcagcaac agaaaagagc aacgtggttc ggggatgggt gtttgggtca acaatgaaca
      361 acaaaagcca aagcgtcatt attataaaca actctaccaa cgtggtgatc cgagcttgta
      421 actttgagct gtgtgacaac cccttcttcg ccgtctccaa gccgatggga acacaaaccc
      481 atactatgat attcgataat gcctttaatt gtaccttcga gtatatatct gatgcctttt
      541 cactggatgt tagtgaaaag agcgggaatt tcaaacacct tagagagttc gtctttaaga
      601 acaaagacgg gttcttgtat gtttataagg gataccagcc gattgatgtt gtccgagatt
      661 tgccttccgg gtttaacact cttaaaccaa ttttcaaatt gcctcttggg attaacatca
      721 caaactttcg agctattctt actgcctttt ccccggcaca agatatatgg ggaacttcag
      781 cagcagccta tttcgttggt tatcttaaac ctactacgtt catgctcaaa tacgacgaaa
      841 acgggaccat caccgacgcc gttgattgct cccagaaccc actggctgag cttaaatgta
      901 gcgtcaagtc atttgagatt gacaagggca tttatcagac aagcaatttc cgcgttgtcc
      961 cttccgggga cgttgttcgc tttccaaata tcactaacct ctgcccgttt ggcgaggtgt
     1021 ttaacgctac aaagtttcca tcagtgtatg cttgggagcg gaagaaaatt tccaactgcg
     1081 ttgcagacta tagcgtcctc tacaattcca ccttcttttc tacatttaaa tgctacggcg
     1141 tcagtgcgac taaactgaat gacctttgtt tcagcaatgt gtacgccgac tcattcgtgg
     1201 tgaagggaga tgatgtcagg cagatcgctc ctgggcaaac cggagtaatc gcggactata
     1261 actataagct gcctgatgat tttatgggtt gtgtgctcgc atggaataca cgaaacattg
     1321 acgctacttc caccgggaac tataattata agtaccgata cctcagacac gggaaattga
     1381 gaccttttga acgggacatt tcaaacgttc cattctcacc tgatggcaag ccatgcacgc
     1441 cacccgcttt gaattgctac tggcctctga acgattacgg tttctatacc actaccggga
     1501 tcgggtatca accctaccgc gttgtcgtcc tgagctttga attgcttaat gcgcctgcca
     1561 cagtctgcgg gccaaagctc tcaactgatc tgattaagaa tcaatgcgtc aatttcaact
     1621 tcaatggtct gaccgggact ggggttctca ccccctcttc caagcgcttt caaccattcc
     1681 aacagtttgg ccgagatgtt tcagacttca ctgattctgt aagagatccc aagacgagtg
     1741 agattcttga catcagcccc tgtgcatttg gtggcgtttc cgtgataaca cccggaacta
     1801 atgcttcatc cgaagtcgcc gttctctatc aagatgttaa ttgcaccgac gtgtctaccg
     1861 ctatacacgc cgatcaactc acccccgcct ggcgtattta ttccactggc aacaacgttt
     1921 tccagacaca agccggttgt ctgattggag ccgaacacgt cgacaccagt tatgagtgcg
     1981 acatccccat tggagccgga atctgcgcca gctaccacac tgttagtctc ctgcgatcaa
     2041 ccagtcaaaa gtccattgta gcttacacga tgagcttggg agctgattct agcatagcct
     2101 acagtaacaa caccattgcc atccctacca atttctcaat ttctataact actgaggtca
     2161 tgcccgtcag tatggctaag acttccgtcg attgcaatat gtatatctgc ggagattcca
     2221 cggagtgcgc taacctcttg ttgcaatatg gttccttttg cacacaattg aaccgtgcgt
     2281 tgagtggcat cgccgcagag caagatcgca ataccagaga ggtgttcgcc caggtcaagc
     2341 aaatgtataa aactcctact ctgaagtatt ttggtggttt taacttttca caaattttgc
     2401 cagaccccct taaacctacc aaaaggagct ttatagagga tctgctgttc aataaagtga
     2461 ctctcgctga tgccgggttt atgaaacaat atggagagtg cttgggagac atcaatgcta
     2521 gggaccttat atgtgcccaa aagttcaacg ggctgaccgt gctgccccca ttgctcaccg
     2581 atgatatgat tgccgcgtat acagccgctc tcgtcagcgg aactgccaca gcgggctgga
     2641 ccttcggggc tggcgcggcc cttcaaatcc cctttgcaat gcaaatggcc tatcgcttca
     2701 acggtatcgg agttacccaa aacgtgctct atgaaaatca aaagcagata gcgaatcaat
     2761 ttaacaaggc aatttcacaa atacaagaaa gccttacgac tacatctacc gccctgggca
     2821 agctgcaaga tgtcgtgaat caaaacgctc aagccctgaa tactcttgtg aaacaactgt
     2881 ccagcaactt tggcgcaatt agcagcgtcc tgaatgatat tctgtctcgc cttgataagg
     2941 tcgaggcaga agtccaaatt gaccgcctga ttaccggacg attgcaatca ctccagactt
     3001 atgttacgca acaactgatt cgcgctgctg aaatacgcgc ttctgccaac ctcgcagcga
     3061 ccaaaatgag cgagtgtgtg ttggggcagt ctaagcgcgt ggatttctgt ggcaaaggct
     3121 accatctcat gtcatttcca caagcggctc cacatggtgt ggtctttctc catgttacat
     3181 acgttccatc ccaagagaga aactttacaa ctgcgccagc catttgtcac gaaggcaagg
     3241 cgtacttccc cagggaaggg gtgttcgttt tcaatggaac atcctggttt atcactcaac
     3301 ggaatttctt tagtccccaa atcatcacga ccgacaatac cttcgtgtcc ggcaattgtg
     3361 atgtcgtcat tggaattata aacaacaccg tttacgatcc gctccagcca gaactcgaca
     3421 gtttcaaaga ggagcttgat aagtatttca agaaccacac cagtccagat gttgaccttg
     3481 gagatatttc cggtataaat gcaagtgtcg tgaatataca gaaggaaatc gacaggctga
     3541 atgaggtggc aaagaacttg aatgagagtt tgatagacct tcaagagctg gggaagtacg
     3601 aacaatatat taagtggccg tggtatgttt ggctcggatt tatagctggt ctgatcgcta
     3661 ttgttatggt caccattctg ctgtgttgca tgaccagttg ttgtagttgt cttaaaggcg
     3721 cctgttcctg tggaagttgt tgtaaataaa ggatcctcta gaagttgtct cctcctgcac
     3781 tgactgactg atacaatcga tttctggatc cgcaggccta atcaacctct ggattacaaa
     3841 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3901 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3961 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4021 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4081 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4141 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4201 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     4261 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4321 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4381 cggatctccc tttgggccgc ctccccgcct gtctagcttg actgactgag atacagcgta
     4441 ccttcagctc acagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     4501 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     4561 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     4621 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtattggccc
     4681 atctctatcg gtatcgtagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     4741 ttgtgcccct cgggccggat tgctatctac cggcattggc gcagaaaaaa atgcctgatg
     4801 cgacgctgcg cgtcttatac tcccacatat gccagattca gcaacggata cggcttcccc
     4861 aacttgccca cttccatacg tgtcctcctt accagaaatt tatccttaag gtcgtcagct
     4921 atcctgcagg cgatctctcg atttcgatca agacattcct ttaatggtct tttctggaca
     4981 ccactagggg tcagaagtag ttcatcaaac tttcttccct ccctaatctc attggttacc
     5041 ttgggctatc gaaacttaat taagcgatct gcatctcaat tagtcagcaa ccatagtccc
     5101 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5161 tcgctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5221 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaggag gtagccaaca
     5281 tgattgaaca agatggattg cacgcaggtt ctcccgccgc ttgggtggag aggctattcg
     5341 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     5401 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc
     5461 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     5521 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     5581 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     5641 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     5701 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     5761 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg atgcccgacg
     5821 gcgaggatct cgtcgtgacc cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5881 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5941 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     6001 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     6061 acgagttctt ctagtatgta agccctgtgc cttctagttg ccagccatct gttgtttgcc
     6121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     6241 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     6301 gctctatggt taattaacca gtcaagtcag ctacttggcg agatcgactt gtctgggttt
     6361 cgactacgct cagaattgcg tcagtcaagt tcgatctggt ccttgctatt gcacccgttc
     6421 tccgattacg agtttcattt aaatcatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6481 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6541 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6601 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6661 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6721 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6781 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6841 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6901 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6961 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7021 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7081 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7141 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7201 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7261 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7321 catagttgca tttaaatttc cgaactctcc aaggccctcg tcggaaaatc ttcaaacctt
     7381 tcgtccgatc catcttgcag gctacctctc gaacgaacta tcgcaagtct cttggccggc
     7441 cttgcgcctt ggctattgct tggcagcgcc tatcgccagg tattactcca atcccgaata
     7501 tccgagatcg ggatcacccg agagaagttc aacctacatc ctcaatcccg atctatccga
     7561 gatccgagga atatcgaaat cggggcgcgc ctggtgtacc gagaacgatc ctctcagtgc
     7621 gagtctcgac gatccatatc gttgcttggc agtcagccag tcggaatcca gcttgggacc
     7681 caggaagtcc aatcgtcaga tattgtactc aagcctggtc acggcagcgt accgatctgt
     7741 ttaaacctag atattgatag tctgatcggt caacgtataa tcgagtccta gcttttgcaa
     7801 acatctatca agagacagga tcagcaggag gctttcgcat gagtattcaa catttccgtg
     7861 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7921 tggtgaaagt aaaagatgct gaagatcagt tgggtgcgcg agtgggttac atcgaactgg
     7981 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgcttt ccaatgatga
     8041 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8101 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtattca ccagtcacag
     8161 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8221 gtgataacac tgcggccaac ttacttctga caacgattgg aggaccgaag gagctaaccg
     8281 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8341 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacct
     8401 tgcgtaaact attaactggc gaactactta ctctagcttc ccggcaacag ttgatagact
     8461 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8521 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8581 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8641 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8701 cgattctagg tgcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact
     8761 cccacatatg ccagattcag caacggatac ggcttcccca acttgcccac ttccatacgt
     8821 gtcctcctta ccagaaattt atccttaaga tcccgaatcg tttaaactcg actctggctc
     8881 tatcgaatct ccgtcgtttc gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg
     8941 gcatcgaatc tcgtcagcta tcgtcagctt acctttttgg cagcgatcgc ggctcccgac
     9001 atcttggacc attagctcca caggtatctt cttccctcta gtggtcataa cagcagcttc
     9061 agctacctct caattcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
     9121 tatcaatcgt tgcgttacac acacaaaaaa ccaacacaca tccatcttcg atggatagcg
     9181 attttattat ctaactgctg atcgagtgta gccagatcta gtaatcaatt acggggtcat
     9241 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     9301 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     9361 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     9421 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     9481 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     9541 acatctacgt attagtcatc gctattacca tgctgatgcg gttttggcag tacatcaatg
     9601 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     9661 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     9721 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
     9781 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     9841 accgggaccg atccagcctc ccctcgaagc ttacatgtgg taccgagctc ggatcctgag
     9901 aacttcaggg tgagtctatg ggacccttga tgttttcttt ccccttcttt tctatggtta
     9961 agttcatgtc ataggaaggg gagaagtaac agggtacaca tattgaccaa atcagggtaa
    10021 ttttgcattt gtaattttaa aaaatgcttt cttcttttaa tatacttttt tgtttatctt
    10081 atttctaata ctttccctaa tctctttctt tcagggcaat aatgatacaa tgtatcatgc
    10141 ctctttgcac cattctaaag aataacagtg ataatttctg ggttaaggca atagcaatat
    10201 ttctgcatat aaatatttct gcatataaat tgtaactgat gtaagaggtt tcatattgct
    10261 aatagcagct acaatccagc taccattctg cttttatttt atggttggga taaggctgga
    10321 ttattctgag tccaagctag gcccttttgc taatcatgtt catacctctt atcttcctcc
    10381 cacagctcct gggcaacgtg ctggtctg
//
